Development and Evaluation of Epigenetic Regulation of Sucrose Metabolism in Saccharomyces cerevisiae by Using COBRA Technique and Selected CpG Islands in HXT10 and SUC2 genes

Year 2019, Volume: 5 Issue: 1, 50 - 55, 27.06.2019

Burak Alaylar Medine Güllüce Mehmet Karadayı

Abstract

Epigenetics mostly focuses on heritable
changes in gene expression that does not contain changes to the underlying
primary DNA sequences; an alteration in phenotype without an alteration in
genotype. Among various cellular mechanisms, especially DNA methylation plays a
central role in many epigenetic processes. Therefore, it has been the most
studied epigenetic mechanism and many special techniques for assessment of
changes in DNA methylation patterns have been developed. Combined bisulfite
restriction analysis (COBRA) is one of these commonly used techniques. Although
many studies have been conducted to get information about epigenetic regulation
mechanisms in mammalian cell by using COBRA, there is still limited information
on the the epigenetic programs and related pathways in other organisms.  

Keywords

COBRA, DNA methylation

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