ANALYSIS OF THIOLE/DISULFIDE HOMEOSTASIS IN PATIENTS WITH DECUBITUS ULCER

Abstract

Aim: There is a growing body of literature that recognises the importance of decubitus ulcer. Decubitus ulcer is tissue necrosis and ulceration that inevitably occurs in tissues exposed to pressure for a long time. Reoxygenation of ischemic tissue results in the formation of reactive oxygen metabolites, during ischemia/reperfusion. Thiols are antioxidant substances belonging to the mercaptan group. This study aims to contribute to this growing area of research by exploring thiol/disulfide levels, a new oxidative stress parameter, in patients with decubitus ulcer. Materials and Method: This study consists of two groups, one is the control group and the other is the patient group. While the control group consists of 50 healthy individuals, the patient group consists of 50 patients with decubitus ulcer. Native thiol and total thiol levels were measured by spectrophotometric method. Data management and analysis were performed using SPSS software (version 22). Results: While the average native thiol, total thiol level, reduced thiol ratio, and thiol oxidation reduction ratio levels in the patient group were lower than the control group; disulfide and oxidized thiol levels were higher than the control group Conclusion: Total thiols, native (free) thiols and disulfide levels were significantly changed in the patient group compared to healthy individuals. The most striking result to emerge from the data is that plasma dynamic thiol/disulfide levels alter and shift to the disufide side in patients with decubitus ulcers.

Keywords

• Thiol/disulfide homeostasis
• decubitus ulcer
• oxidative stress
• tissue damage

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