Antidiabetic and Antioxidant Effects of Bryonia multiflora Boiss. & Heldr. in a Rat Model of Streptozotocin-Induced Diabetes

Abstract

Objective: This aim of this study is to investigate the antidiabetic, antioxidant and hypolipidemic potential of Bryonia multiflora Boiss. & Heldr. in streptozotocin (STZ) induced diabetes in rats. Material and Methods: During 21 days, control group (NC) and diabetes control (DC) were fed only with food and water, while diabetes acarbose group (DAC) was fed with 20 mg / kg of acarbose. The DB1, DB2, DB3 groups were fed with 100 mg / kg, 200 mg/kg and 400 mg / kg Bryonia multiflora plant extract, respectively. The body weight and biochemical parameters glucose level, insulin level, glycosylated hemoglobin, total cholesterol, triglyceride, aminotransferase (ALT), lactate dehydrogenase (LDH), C-peptide, LDL- cholesterol (LDL-c), HDL-cholesterol (HDL-c), creatinine (CRE), blood urea nitrogen (BUN), urea (Ure) and antioxidant parameters were examined for all treated groups and compared against diabetic control group and normal control group. Results: Bryonia multiflora (BM) plant extract (100 mg/kg, 200 mg/kg and 400 mg/kg) that administered in respective groups of diabetic animals for 21 days significantly reduced the blood glucose level and glycosylated hemoglobin ( HbA1c) levels in streptozotocin induced diabetic rats. While the levels of insulin and C-peptide of DC group were found significantly lower (p <0.05) compared to NC group, a significant increase (p < 0.05) in insulin and c- peptide level were determined in the groups treated with plant extracts compared to DC group. According to the results; significantly higher levels have been observed in DC group serum ALT, AST, BUN, and CRE compared to NC group (p<0.05), while declines have been observed in groups treated with BM extract (p<0.05). There has been decline in VLDL, cholesterol, and triglycerite levels in the plant extract applied groups (p<0.05), while HDL levels increased (p<0.05). On the other hand, MDA levels increased while GSH and SOD levels declined as DC group compared to the control group. MDA levels significantly declined (p<0.05) while SOD and GSH levels significantly increased in therapeutic groups treated with plant extracts. Conclusion: The results indicate that BM extract have antidiabetic effects by regulating antioxidant activities thereby improving the function of β-cells maintaining normal insulin and glucose levels. Thus the investigation results that Bryonia multiflora has significant antidiabetic, antioxidant activity.

Keywords

• Antidiabetic
• Antioxidant
• Bryonia multiflora
• Diabetes mellitus
• Oxidative stress
• Rats

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