Antihistamin Levosetirizin Dihidroklorürün Sitogenetik Belirteçler Üzerindeki Etkisi

Abstract

Levosetirizin dihidroklorür, alerjik hastalıkların tedavisinde kullanılan ikinci nesil bir antihistaminik olan Alerinit'in aktif bileşenidir. Bu çalışma, insan periferal lenfositlerinde kromozomal anomali (CA) ve mikronükleus (MN) testleri kullanılarak levosetirizinin olası genotoksik ve sitotoksik etkilerini belirlemek için yapılmıştır. Bu çalışmada, hücre kültürleri 24 ve 48 saat boyunca Levocetirizine ön çalışması ile belirlenen 2, 4, 8 ve 16 μg / ml konsantrasyonları ile muamele edilmiştir. Çalışmamız sonucunda Levosetirizin'in bütün konsantrasyonlarında, CA / hücre hızı, anormal hücre yüzdesi ve mitotik indeks değerleri, MN frekansı, binükleer mikronükleus hücre hızı ve nükleer bölünme indeksi değerlerinde kontrol ile karşılaştırıldığında anlamlı bir değişikliğe neden olmadığını gözlemledik. Bu araştırmaya göre; levosetirizin dihidroklorürün genotoksik ve sitotoksik etkisi yoktur.

Keywords

• Kromozamal aberasyon
• Sitotoksisite
• Geneotoksisite
• Mikronükleus
• Levosetirizin dihidroklorür

Effect of Antihistamine Levocetirizine Dihydrochloride on Cytogenetic Markers

Abstract

Levocetirizine dihydrochloride is the active ingredient of Alerinit, a second-generation antihistamine, used in the treatment of allergic diseases. This study was carried out to determine the probable genotoxic and cytotoxic effects of levocetirizine using chromosomal abnormality (CA) and micronucleus (MN) tests in human peripheral lymphocytes. In this study, cell cultures were treated with 2, 4, 8 and 16 μg / ml concentrations determined by preliminary study of Levocetirizine during 24 and 48 hours. As a result of our study, we observed that Levocetirizine does not cause any significant change compared to control in CA / Cell rate, abnormal cell percentage and mitotic index values, MN frequency, binuclear micronucleus cell rate and nuclear division index values at within the all studied concentrations and treatment periods. According to this research; levocetirizine dihydrochloride has no genotoxic and cytotoxic effects.

Keywords

• Chromosome Aberration
• Cytotoxicity
• Genotoxicity
• Micronucleus
• Levocetirizine Dihydrochloride

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