Investigation of Dynamic Thiol Disulfide Homeostasis in Acute Respiratory Failure Patients in Intensive Care Unit

Abstract

Background/aim:Thiols are antioxidant agents which belong to mercaptan group consisting of sulfur and carbon containing sulfhydryl (-SH). Thiol levels, in the case of elevated oxidative stress, are lowered by their use in neutralization of reactive oxygen molecules. Materials and methods:It was aimed to investigate thiol and disulphide blood levels or thiol/disulphide ratio as a marker of total oxidant status which plays a role in the pathogenesis of inflammatory diseases that may cause respiratory failure. Results:The study included 98 patients (58 males, 40 females) who have partial oxygen pressure (PaO2)<60 mmHg or partial carbon dioxide pressure (PaCO2)>45 mmHg in arterial blood in the intensive care unit and 98 healthy volunteers. Total thiol and native thiol levels were measured by spectrophotometric method. Total thiol (270 ± 99.81), native thiol (203.90 ± 103.41) and disulphide (33.10±12.42) levels of the patient group were significantly lower (p <0.001) than total thiol (423.62 ± 70.3), native thiol (307.13 ± 57.73) and disulphide (58.24±27.21) of the control group levels. There is no significant difference between native thiol / total thiol, disulphide/ total thiol and disulphide /native thiol ratios. Conclusion:Thiol and disulphide blood levels or thiol / disulphide ratio can guide us as a prognostic test in acute respiratory failure patients.

Keywords

• Native thiol
• Oxidative stress
• Respiratory failure
• Thiol/disulphide homeostasis
• Total thiol

References

1. Referans 1 Yarkın T. Solunum Yetmezliği: Fizyopatoloji ve Klinik Yaklaşım. Toraks Dergisi.2000;2:76-84.
2. Referans 2 Frutos-Vivar F, Nin N, Esteban A. Epidemiology of acute lung injury and acute respiratory distress syndrome.CurrOpin Crit Care. 2004;10(1):1-6.
3. Referans 3 Esteban A, Anzueto A, Frutos F, Alía I, Brochard L, Stewart TE, Benito S, Epstein SK, Apezteguía C, Nightingale P, Arroliga AC, Tobin MJ; Mechanical Ventilation International Study Group. Characteristics and outcomes in adult patients receiving mechanical ventilation: a 28-day international study.JAMA. 2002;16:287(3):345-355.
4. Referans 4 Becatti M, Taddei N, Fiorillo C. Oxidative stress management during non invasive ventilation in acute respiratory failure.Intern Emerg Med. 2018;13:141–142.
5. Referans 5 Özcan O, Erdal H, Çakırca G, Yöndem Z. Oxidative stress and its impacts on intracellular lipids, proteins and DNA. Journal of Clinical and Experimental Investigations. 2015;6(3):331-336.
6. Referans 6 Gutteridge JMC, Quinlan GJ, Mumby S, Heath A, Evans TW. Primary plasma antioxidants in adult respiratory distress syndrome patients: changes in iron-oxidizing, iron-binding and free radical-scavenging proteins. J. Lab. Clin. Med. 1994;124:263-273.
7. Referans 7 Hecker L. Mechanisms and consequences of oxidative stress in lung disease: therapeutic implications for an aging populace. Am J Physiol Lung Cell Mol Physiol. 2018;314(4):642–653.
8. Referans 8 Quinlan GJ. Lamb NJ, Tilley R, Evans TW, Gutteridge JM.Plasma hypoxanthine levels in ARDS: implications for oxidative stress, morbidity, and mortality. American Journal of Respiratory and Critical Care Medicine. 1997;155(2):479–484.

9. Referans 9 Gürsel G. Akut Solunum Sıkıntısı Sendromu(ARDS).Yoğun Bakım Dergisi. 2002;2(2):96-107.
10. Referans 10 Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr. 2000;72(2 Suppl.):653–669.
11. Referans 11 Matteucci E, Giampietro O. Thiol signalling network with an eye to diabetes. Molecules 2010;15(12):8890–903.
12. Referans 12 Go YM, Jones DP. Cysteine/cystine redox signaling in cardiovascular disease. Free RadicBiol Med. 2011;15:50(4):495-509.
13. Referans 13 Prabhu A, Sarcar B, Kahali S, Yuan Z, Johnson JJ, Adam KP, et al. Cysteine catabolism: a novel metabolic pathway contributing to glioblastoma growth. Cancer Res 2014;74(3):787–96.
14. Referans 14 Tetik S, Ahmad S, Alturfan AA, Fresko I, Disbudak M, Sahin Y, Aksoy H, Yardimci KT. Determination of oxidant stress in plasma of rheumatoid arthritis and primary osteoarthritis patients. Indian J BiochemBiophys. 2010;47(6):353-358.
15. Referans 15 Calabrese V, Lodi R, Tonon C, D'Agata V, Sapienza M, Scapagnini G, Mangiameli A, Pennisi G, Stella AM, Butterfield DA. Oxidative stress, mitochondrial dysfunction and cellular stress response in Friedreich's ataxia. J Neurol Sci. 2005;15:233(1-2):145-62.
16. Referans 16 Steele ML, Fuller S, Maczurek AE, Kersaitis C, Ooi L, Münch G. Chronic inflammation alters production and release of glutathione and related thiols in human U373 astroglial cells.Cell Mol Neurobiol. 2013;33(1):19-30.
17. Referans 17 Kuo LM, Kuo CY, Lin CY, Hung MF, Shen JJ, Hwang TL.Intracellular glutathione depletion by oridonin leads to apoptosis in hepatic stellate cells. .Molecules. 2014;18:19(3):3327-3344.
18. Referans 18 Frutos-Vivar F, Nin N, Esteban A. Epidemiology of acute lung injury and acute respiratory distress syndrome.CurrOpin Crit Care. 2004;10(1):1-6.
19. Referans 19 Esteban A, Anzueto A, Frutos F, Alía I, Brochard L, Stewart TE, Benito S, Epstein SK, Apezteguía C, Nightingale P, Arroliga AC, Tobin MJ; Mechanical Ventilation International Study Group. Characteristics and outcomes in adult patients receiving mechanical ventilation: a 28-day international study.JAMA. 2002;16:287(3):345-355.
20. Referans 20 Quinlan GJ. Lamb NJ, Tilley R, Evans TW, Gutteridge JM.Plasma hypoxanthine levels in ARDS: implications for oxidative stress, morbidity, and mortality. American Journal of Respiratory and Critical Care Medicine. 1997;155(2):479–484.
21. Referans 21 Baldwin S, Grum C, Boxer L, Simon R, Ketai L, Devall L. Oxidant activity in expired breath of patients with adult respiratory distress syndrome. The Lancet. 1986;327(8471):11–14.
22. Referans 22 Lenz AG, Jorens PG, Meyer B, Backer WD, Overveld FV, BossaertL,Maier KL. Oxidatively modified proteins in bronchoalveoler lavage fluid of patients with ARDS and patients at-risk for ARDS. Eur Respir J 1999;13: 169-174.
23. Referans 23 Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clinical Biochem. 2014;47(18):326–332.
24. Referans 24 Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr. 2000;72(2 Suppl.):653–669.
25. Referans 25 Turell L, Radi R, Alvarez B. The thiol pool in human plasma: The central contribution of albumin to redox processes. Free Radic. Biol. Med. 2013; 65: 244–253.
26. Referans 26 Jones DP, Liang Y. Measuring the Poise of Thiol/Disulfide Couples in vivo. Free RadicBiol Med. 2009; 47(10): 1329–1338.
27. Referans 27 Parlak ES, Alisik M, Hezer H, Karalezli A, Hasanoglu HC, Erel O. Evaluation of dynamic thiol/disulfide redox state in community-acquired pneumonia. Saudi Med J 2018;39(5): 495-499.
28. Referans 28 Babaoglu E, Kılıc H, Hezer H, Dag O, Parlak E, Senturk A, Karalezlı A, Alısık M, Erel O, Hasanoglu H.C. European Review for Medical and Pharmacological Sciences. Comparison of thiol/disulphide homeostasis parameters in patients with COPD, asthma and ACOS. 2016;20:1537-1543.
29. Referans 29 Ahmad A, Shameem M, Husain Q. Altered oxidant-antioxidant levels in the disease prognosis of chronic obstructive pulmonary disease. Int J Tuberc Lung Dis. 2013;17(8):1104-1109.
30. Referans 30 Dilek F, Ozkaya E, Kocyigit A, Yazici M, Guler EM, Dundaroz MR. Plasma total thiol pool in children with asthma: Modulation during montelukast monotherapy. International Journal of Immunopathology and Pharmacology. 2016;29(1):84–89.
31. Referans 31 Temel MT, Demiryurek S, Temel L, Saracaloglu A, Eke N, Baysalman E, Mammadov A, Coskun ME, Demiryurek AT. Dynamic thiol/disulfide homeostasis in children with communityacquired Pneumonia. Pediatr Int. 2019;61(3):252-257
32. Referans 32 Sonmez MG, Kozanhan B, Deniz CD, Iyisoy MS, Kilinc MT, Ecer G, Ozturk A, Neselioglu S, Erel O. Dynamic thiol/disulfide homeostasis as a novel indicator of oxidative stress in patients with urolithiasis. Investig Clin Urol. 2019;60:258-266.
33. Referans 33 Raut AM, Suryakar AN, Mhaısekar D. A study of oxidative stress, thiol proteins and role of vitamin E supplementation in chronic obstructive pulmonary disease (COPD). Al Ameen J Med Sci 2013;6:134-137.
34. Referans 34 Erden ES, Motor S, Ustun I, Demirkose M, Yuksel R, Okur R, Oktar S, Yakar Y, Sungur S, Gokce C. Investigation of Bisphenol A as an endocrine disruptor, total thiol, malondialdehyde, and C-reactive protein levels in chronic obstructive pulmonary disease. Eur Rev Med Pharmacol Sci. 2014;18(22):3477-3483.
35. Referans 35 Gündüzöz M, Birgin İS, Tutkun L, Büyükşekerci M, Pinar Çetintepe S, Bal C, Alişik M, Erdoğan S, Yilmaz H, Erel Ö. A new potential biomarker in early diagnosis of firefighter lung function impairment: dynamic thiol/disulphide homeostasis. Cent Eur J Public Health. 2018;26(3):190-194.