Antidiabetic and Antioxidant Effects of Bryonia multiflora Boiss. & Heldr. in a Rat Model of Streptozotocin-Induced Diabetes

Year 2023, Volume: 4 Issue: 1, 453 - 476, 20.07.2023

Elif Ebru Alkan , İsmail Çelik , Bedia Bati

https://doi.org/10.46871/eams.1287621

Cited By: 2

Abstract

Objective: This aim of this study is to investigate the antidiabetic, antioxidant and hypolipidemic potential of Bryonia multiflora Boiss. & Heldr. in streptozotocin (STZ) induced diabetes in rats. Material and Methods: During 21 days, control group (NC) and diabetes control (DC) were fed only with food and water, while diabetes acarbose group (DAC) was fed with 20 mg / kg of acarbose. The DB1, DB2, DB3 groups were fed with 100 mg / kg, 200 mg/kg and 400 mg / kg Bryonia multiflora plant extract, respectively. The body weight and biochemical parameters glucose level, insulin level, glycosylated hemoglobin, total cholesterol, triglyceride, aminotransferase (ALT), lactate dehydrogenase (LDH), C-peptide, LDL- cholesterol (LDL-c), HDL-cholesterol (HDL-c), creatinine (CRE), blood urea nitrogen (BUN), urea (Ure) and antioxidant parameters were examined for all treated groups and compared against diabetic control group and normal control group. Results: Bryonia multiflora (BM) plant extract (100 mg/kg, 200 mg/kg and 400 mg/kg) that administered in respective groups of diabetic animals for 21 days significantly reduced the blood glucose level and glycosylated hemoglobin ( HbA1c) levels in streptozotocin induced diabetic rats. While the levels of insulin and C-peptide of DC group were found significantly lower (p <0.05) compared to NC group, a significant increase (p < 0.05) in insulin and c- peptide level were determined in the groups treated with plant extracts compared to DC group. According to the results; significantly higher levels have been observed in DC group serum ALT, AST, BUN, and CRE compared to NC group (p<0.05), while declines have been observed in groups treated with BM extract (p<0.05). There has been decline in VLDL, cholesterol, and triglycerite levels in the plant extract applied groups (p<0.05), while HDL levels increased (p<0.05). On the other hand, MDA levels increased while GSH and SOD levels declined as DC group compared to the control group. MDA levels significantly declined (p<0.05) while SOD and GSH levels significantly increased in therapeutic groups treated with plant extracts. Conclusion: The results indicate that BM extract have antidiabetic effects by regulating antioxidant activities thereby improving the function of β-cells maintaining normal insulin and glucose levels. Thus the investigation results that Bryonia multiflora has significant antidiabetic, antioxidant activity.

Keywords

Antidiabetic, Antioxidant, Bryonia multiflora, Diabetes mellitus, Oxidative stress, Rats

Project Number

2014-FBE-D151

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