Tatlısu Midyesi Unio Terminalis’te Bakırın 96 Saatlik LC50 Değeri ile Birikim Düzeylerinin Belirlenmesi

Abstract

Bu çalışmada tatlı su midyesi Unio terminalis’te eser ağır metallerden bakırın 96 saatlik LC50 değeri ile 96 saatlik LC50 değerinin %25, %50 ve %100’ne karşılık gelen derişimlerinin 96 saat süreyle etkisinde total birikim düzeylerinin belirlenmesi amaçlanmıştır. Midye örneklerinin bakır analizinde ICP-MS kullanılmıştır. U. terminalis’te bakırın 96 saatlik LC50 değeri 4.65 ppm olarak belirlenmiştir. Daha sonra midyeler 96 saat süreyle, bakırın bu derişimin %25, %50 ve %100’ne karşılık gelen 1, 2 ve 4 ppm’lik derişimlerinin etkisinde bırakılmış ve deney süresi sonunda total birikim düzeyleri sırasıyla 56 µg Cu/g k.a., 77 µg Cu/g k.a. ve 28 µg Cu/g k.a. olarak tespit edilmiştir. İncelenen tüm derişimlerin etkisinde, birikim düzeylerinin kontrol grubuna oranla önemli düzeyde artış gösterdiği saptanmıştır. 1 ve 2 ppm’lik derişimlerin etkisindeki birikim düzeyleri, incelenen yüksek derişimin etkisindeki birikime oranla daha fazla olmuştur

Keywords

• Bakır, LC50, Birikim, Unio terminalis, Tatlısu Midyesi

Determination of 96 h LC50 Value of Copper and Its Total Body Accumulation during the Same Perod in Unio terminalis

Abstract

In this study, Unio terminalis was exposed to 25%, 50% and 100% of its 96 h LC50 value of copper over 96 hours after determining the 96 h LC50 value for this metal. The 96 h LC50 value of copper to this species was determined as 4.65 ppm Cu and hence the species was exposed to 1.0, 2.0 and 4.0 ppm Cu for 96 hours. Tissue analysis of copper was carried out using ICP-MS techniques. Total body accumulation of copper were 56 µg Cu/g d.w., 77µg Cu/g d.w. and 28µg Cu/g d.w. for 1.0, 2.0 and 4.0 ppm Cu exposures respectively after 96 hours. Copper levels were significantly higher in animals exposed to copper compared with the control. Accumulation of copper, however, was lower at the highest Cu concentration tested compared the lower concentrations.

Keywords

• Copper, LC50, Accumulation, Unio termialis, Freshwater mussel

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