Gökkuşağı Alabalığı (Oncorhynchus mykiss) ve Aynalı Sazan (Cyprinus carpio) Spermasının Glukoz Tabanlı Sulandırıcı ile Dondurulması

Year 2005, Volume: 1 Issue: 1, - , 04.01.2005

Yusuf Bozkurt Selçuk Seçer Necmettin Tekin Ergun Akçay

Abstract

Bu çalışmada, gökkuşağı alabalığı (Oncorhynchus mykiss) ve aynalı sazan (Cyprinus carpio) spermasının dondurulması araştırıldı. Spermatolojik özelliklerin belirlenmesinin ardından, ejakulatlar glukoz tabanlı sulandırıcı ile sulandırıldı. Sulandırılan sperma 0,5 ml’lik payetlere çekilerek sıvı azot buharında (-196 °C) 10 dakika süre ile donduruldu ve sıvı azot içerisine aktarıldı. Payetler 30 °C su banyosunda 30 saniye süre ile çözüldü. Gökkuşağı alabalığında çözülme sonu en iyi motilite (%), canlılık süresi (s) ve döl verimi yüzdesi sırasıyla % 57, 96 s. ve % 68,5 olarak elde edilirken aynı özellikler aynalı sazanda sırasıyla % 64, 218 s. ve % 35 olarak belirlendi.

Keywords

Gökkuşağı alabalığı (Oncorhynchus mykiss), aynalı sazan (Cyprinus carpio), sperma, sulandırıcı, kryoprezervasyon, payet, motilite, döllenme

Cryopreservat on of Rainbow Trout (Oncorhynchus mykiss) and Mirror Carp (Cyprinus carpio) Sperm with Glucose Based Extender

Abstract

In this study, cryopreservation of rainbow trout (Oncorhynchus mykiss) and mirror carp (Cyprinus carpio) spermatozoa was investigated. Following determination of the spermatological properties, the pooled ejaculates were diluted with glucose based extender. Diluted sperm was packaged in 0.5 ml straws and frozen in liquid nitrogen vapour (-196 °C) for 10 minutes and plunged into the liquid nitrogen. The straws were thawed in a water bath at 30 ºC for 30 seconds. In rainbow trout, the best post-thaw motility (%), post-thaw movement duration (s) and post-thaw fertilization results (%) were obtained as 57 %, 96 s. and 68.5 % while the same properties were determined as 64 %, 218 s. and 35 % in mirror carp respectively

Keywords

Rainbow trout (Oncorhynchus mykiss), mirror carp (Cyprinus carpio), sperm, extender, cryopreservation, straw, motility, fertilization

References

• Akçay, E., Bozkurt, Y., Kayam, S. 2002. Cryopreservation of mirror carp (Cyprinus carpio L. 1758) sperm : with emphasis on post-thaw motility. In: 1st Int. Cong. Aquacult.-Fish. Technol. Environ. Management., Athens, Greece p. 5.
• Akçay, E., Bozkurt Y., Seçer S., Tekin, N. 2004a. Cryopreservation of mirror carp semen Turk. J. Vet. Anim. Sci. 28 (4) 837-843.
• Akçay, E., Bozkurt, Y., Seçer, S. Tekin, N. 2004b. Pelet yöntemiyle dondurulan gökku"aA! alabal!A! spermas!nda gliserol ilavelerinin fertilizasyon üzerine etkisi. 3. Ulusal Reprodüksiyon ve Sun’i Tohumlama Kongresi, 30 Eylül - 02 Ekim Antalya, 104.
• Benau, D., Terner, C. 1980. Initiation, prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Res., 3: 247-257.
• Billard, R. 1992. Reproduction in rainbow trout. Aquaculture, 100: 263-298.
• Bozkurt, Y., Seçer, S. 2004. Aynal! sazan (Cyprinus carpio L. 1758) spermas!n!n baz! spermatolojik özelliklerinin belirlenmesi ve farkl! suland!r!c!lar ile k!sa süreli saklanmas!. 3. Ulusal Reprodüksiyon ve Sun’i Tohumlama Kongresi, 30 Eylül - 02 Ekim Antalya, 106.
• Bozkurt, Y., Akçay, E., Tekin, N., Seçer, S. 2005a. Effect of freezing techniques, extenders and cryoprotectants on the fertilization rate of frozen rainbow trout (Oncorhynchus mykiss) sperm. The Israeli Journal of AquacultureBamidgeh, 57 (2) 125-130.
• Bozkurt, Y., Seçer, S. 2005b. Effect of short-term preservation of mirror carp (Cyprinus carpio) semen on motility, fertilization and hatching rates. The Israeli Journal of Aquaculture-Bamidgeh, 57 (3) 207-212.
• Ciereszko, A., Dabrowski, K. 1993. Estimation of sperm concentration of rainbow trout, whitefish and yellow perch using a spectrophotometric technique. Aquaculture, 109: 367-373.
• Geffen, A.J., Evans, J. 2000. Sperm traits and fertilization success of male and sex reversed female rainbow trout., Aquaculture, 182 : 61-72.
• Horvath, A., Miskolczi, E., Urbanyi, B. 2003. Cryopreservation of common carp sperm. Aquat. Living Resour.,16: 457-460.
• Lahnsteiner, F., Patzner, R.A., Weismann, T. 1993. Energy resources of spermatozoa of the rainbow trout, Oncorhynchus mykiss. (Pisces, Teleostei). Reprod. Nutr. Dev., 33: 349-360.
• Lahnsteiner, F., Weismann, T., Patzner, R.A.1997. Methanol as cryoprotectant and the suitability of 1.2 ml and 5 ml straws for cryopreservation of sperm from salmonid fishes. Aquacult. Res., 28: 471-479.
• Levanduski, M.J., Cloud, J.G. 1988. Rainbow trout (Salmo gairdneri) sperm: effect of non-motile sperm on fertility. Aquaculture, 75: 171-179.
• Linhart, O., Rodina, M., Cosson, J. 2000. Cryopreservation of sperm in common carp Cyprinus carpio : sperm motility and hatching success of embryos. Cryobiology, 41: 241-250.
• Lubzens, E., Rothbard, S. Hadani, A. 1993. Cryopreservation and viability of spermatozoa from the ornamental Japanese carp (Nishikigoi). The Israeli Journal of Aquaculture-Bamidgeh, 45: 169-174.
• McNiven, M.A., Gallant, R.K., Richardson, G.F. 1993. Dimethyl-acetamide as a cryoprotectant for rainbow trout spermatozoa. Theriogenology, 40: 943-948.
• Munkittrick, K.R., Moccia, R.D. 1987. Seasonal changes in the quality of rainbow trout (Salmo gairdneri) sperm: effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma constituents. Aquaculture, 64: 147-156.
• Scott, A.P. and S.M. Baynes, 1980. A review of the biology, handling and storage of salmonid spermatozoa. J. Fish Biol. 17 : 707-739.
• Seçer, S., Tekin, N., Bozkurt, Y., Bukan, N., Akçay, E. 2004. Correlation between biochemical and spermatological parameters in rainbow trout (Oncorhynchus mykiss) semen. The Israeli Journal of Aquaculture-Bamidgeh, 56 (4) 274-280.
• Stoss, J. 1983. Fish gamete preservation and spermatozoa physiology. In: Fish physiology, Volume 9B. W.S. Hoar, D.J. Randall and E.M. Donaldson, editors. Academic Press, New York, 305-350.
• Tekin, N., Seçer, S., Akçay, E., Bozkurt, Y. 2003a. Cryopreservation of rainbow trout (Oncorhynchus mykiss) semen. The Israeli Journal of Aquaculture-Bamidgeh, 55 (3) 208-212.
• Tekin, N., Seçer, S., Akçay, E., Bozkurt, Y., Kayam, S. 2003b. The effect of age on spermatological properties in rainbow trout (Oncorhynchus mykiss W. 1792). Turk. J. Vet. Anim. Sci. 27 (1) 37-44.
• Wheeler, P.A., Thorgard, G.H. 1991. Cryopreservation of rainbow trout sperm in large straws. Aquaculture, 93: 95-100.
• Zhang, X., Liu, Y. 1991. Study of cryopreservation of fish spermatozoa. Acta Sci. Nat. Univ. Norm. Hunanensis. 14 : 255