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First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium

Year 2018, Volume: 14 Issue: 3, 166 - 172, 01.09.2018
https://doi.org/10.22392/egirdir.371581

Abstract



In this study, moribund
salema (Sarpa salpa) individuals from
a public aquarium were investigated to identify the cause of disease. A minor
scale loss and partial skin depigmentation were observed in the diseased fish. Internal
investigation of fish revealed hyperemia
in the liver and the intestines and haemorrhages in the muscle tissues. When the histopathological sections of
tissues were examined, it was determined that the bacterial infection affected especially
the gill epithelium, the intestinal mucosa, the heart muscle and hemopoietic
tissues of the spleen. According to phenotypic and biochemical characteristics
as well as SSU rRNA sequencing results, the isolated bacteria was Vibrio harveyi and consistently seen all
the examined samples of visceral organs including kidney, spleen, liver and
blood. However, we need to emphasize that Vibrio
vulnificus
and Pseudoalteromonas
piscicida
bacteria were also isolated from blood and spleen tissues of one
sick fish among others.

References

  • Austin, B., & Zhang, X. H. (2006). Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Letters in Applied Microbiology, 43(2), 119-124. doi:10.1111/j.1472-765X.2006.01989.x
  • Balebona, M. C., Zorrilla, I., Moriñigo, M. A., & Borrego, J. J. (1998). Survey of bacterial pathologies affecting farmed gilt-head sea bream (Sparus aurata L.) in southwestern Spain from 1990 to 1996. Aquaculture, 166(1), 19-35. doi:10.1016/S0044-8486(98)00282-8
  • Clarridge, J. E. (2004). Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clinical Microbiology Reviews, 17(4), 840-862. doi:10.1128/CMR.17.4.840-862.2004
  • Company, R., Sitj, A., Pujalte, M., Garay, E., & Alvarez‐Pellitero, P. (1999). Bacterial and parasitic pathogens in cultured common dentex, Dentex dentex L. Journal of fish diseases, 22(4), 299-309. doi:10.1046/j.1365-2761.1999.00182.x
  • Culling, C. F. A. (1963). Handbook of histopathological techniques (including museum technique).
  • Gusset, M., & Dick, G. (2011). The global reach of zoos and aquariums in visitor numbers and conservation expenditures. Zoo Biology, 30(5), 566-569. doi:10.1002/zoo.20369
  • Haldar, S., Maharajan, A., Chatterjee, S., Hunter, S., Chowdhury, N., Hinenoya, A., . . . Yamasaki, S. (2010). Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta. Microbiological research, 165(8), 639-648. doi:10.1016/j.micres.2009.12.001
  • Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Paper presented at the Nucleic Acids Symposium Series.
  • Larkin, M. A., Blackshields, G., Brown, N., Chenna, R., McGettigan, P. A., McWilliam, H., . . . Lopez, R. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23(21), 2947-2948.
  • López, J. R., de la Roca, E., Núñez, S., de la Herran, R., Navas, J. I., Manchado, M., . . . Toranzo, A. E. (2009). Identification of Vibrio harveyi isolated from diseased cultured wedge sole Dicologoglossa cuneata. Diseases of Aquatic Organisms, 84, 209-217. doi:10.3354/dao02045
  • Pujalte, M., Sitja-Bobadilla, A., Macián, M., Belloch, C., Alvarez-Pellitero, P., Perez-Sanchez, J., . . . Garay, E. (2003). Virulence and molecular typing of Vibrio harveyi strains isolated from cultured dentex, gilthead sea bream and European sea bass. Systematic and applied microbiology, 26(2), 284-292. doi:10.1078/072320203322346146
  • Qin, Y., Wang, J., Su, Y., Wang, D., & Chen, X. (2006). Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara. Acta Oceanologica Sinica, 25(1).
  • Russell, B., Pollard, D., Mann, B.Q., Buxton, C.D. & Carpenter, K.E. (2014). Sarpa salpa. The IUCN Red List of Threatened Species. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.2014-3.RLTS.T170169A1286510.en.
  • Saeed, M. (1995). Association of Vibrio harveyi with mortalities in cultured marine fish in Kuwait. Aquaculture, 136(1), 21-29. doi:10.1016/0044-8486(95)01045-9
  • Suau, A., Bonnet, R., Sutren, M., Godon, J.-J., Gibson, G. R., Collins, M. D., & Doré, J. (1999). Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Applied and environmental microbiology, 65(11), 4799-4807.
  • Tlusty, M. F., Rhyne, A. L., Kaufman, L., Hutchins, M., Reid, G. M., Andrews, C., . . . Dowd, S. (2013). Opportunities for public aquariums to increase the sustainability of the aquatic animal trade. Zoo Biology, 32(1), 1-12. doi:10.1002/zoo.21019
  • Turgay, E., & Karataş, S. (2016). First Report of Vibrio harveyi Infection in Diseased Common Dentex (Dentex dentex) Cultured in Turkey. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 12(2), 170-176.
  • Whitman, K. A. (2004). Finfish and Shellfish Bacteriology Manual: Techniques and Procedures: Iowa state press.
  • Zhang, Z., Schwartz, S., Wagner, L., & Miller, W. (2000). A greedy algorithm for aligning DNA sequences. Journal of Computational biology, 7(1-2), 203-214. doi:10.1089/10665270050081478
  • Zorrilla, I., Arijo, S., Chabrillon, M., Diaz, P., Martinez‐Manzanares, E., Balebona, M., & Morinigo, M. (2003). Vibrio species isolated from diseased farmed sole, Solea senegalensis (Kaup), and evaluation of the potential virulence role of their extracellular products. Journal of fish diseases, 26(2), 103-108. doi:10.1046/j.1365-2761.2003.00437.x

Bir Deniz Akvaryumunda Salpa Balıklarında (Sarpa salpa) Vibrio harveyi Enfeksiyonu Olgusu: ilk rapor

Year 2018, Volume: 14 Issue: 3, 166 - 172, 01.09.2018
https://doi.org/10.22392/egirdir.371581

Abstract

Bu çalışma, bir deniz
akvaryumundan alınan hasta salpa balıklarında (Sarpa salpa) hastalığa neden olan etken veya etkenleri tanımlamak
üzere yapılmıştır. Hasta balıkların vücudunda hafif şiddette deri renginde
açılma ile birlikte pul kayıpları gözlenmiştir. İç bakıda, karaciğer ve
bağırsak dokularının hiperemik ve kas dokusunun da hemorajik olduğu tespit
edilmiştir. Doku kesitleri histopatolojik olarak incelendiğinde, enfeksiyonun;
özellikle solungaç epitelyumu, bağırsak mukozası, kalp kası ve dalağın hematopoetik
dokusunu etkilediği belirlenmiştir. Fenotipik ve biyokimyasal özellikleri ve
SSU rRNA dizileme sonuçlarına göre; incelenen tüm iç organlardan (böbrek,
dalak, karaciğer) ve kan dokusundan Vibrio
harveyi
bakterisi izole edilmiştir. Buna ek olarak, incelenen balıklardan sadece
birinin kan dokusundan ve dalağından alınan örneklerde Vibrio vulnificus ve Pseudoalteromonas
piscicida
bakterileri de izole edilmiştir.

References

  • Austin, B., & Zhang, X. H. (2006). Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Letters in Applied Microbiology, 43(2), 119-124. doi:10.1111/j.1472-765X.2006.01989.x
  • Balebona, M. C., Zorrilla, I., Moriñigo, M. A., & Borrego, J. J. (1998). Survey of bacterial pathologies affecting farmed gilt-head sea bream (Sparus aurata L.) in southwestern Spain from 1990 to 1996. Aquaculture, 166(1), 19-35. doi:10.1016/S0044-8486(98)00282-8
  • Clarridge, J. E. (2004). Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clinical Microbiology Reviews, 17(4), 840-862. doi:10.1128/CMR.17.4.840-862.2004
  • Company, R., Sitj, A., Pujalte, M., Garay, E., & Alvarez‐Pellitero, P. (1999). Bacterial and parasitic pathogens in cultured common dentex, Dentex dentex L. Journal of fish diseases, 22(4), 299-309. doi:10.1046/j.1365-2761.1999.00182.x
  • Culling, C. F. A. (1963). Handbook of histopathological techniques (including museum technique).
  • Gusset, M., & Dick, G. (2011). The global reach of zoos and aquariums in visitor numbers and conservation expenditures. Zoo Biology, 30(5), 566-569. doi:10.1002/zoo.20369
  • Haldar, S., Maharajan, A., Chatterjee, S., Hunter, S., Chowdhury, N., Hinenoya, A., . . . Yamasaki, S. (2010). Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta. Microbiological research, 165(8), 639-648. doi:10.1016/j.micres.2009.12.001
  • Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Paper presented at the Nucleic Acids Symposium Series.
  • Larkin, M. A., Blackshields, G., Brown, N., Chenna, R., McGettigan, P. A., McWilliam, H., . . . Lopez, R. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23(21), 2947-2948.
  • López, J. R., de la Roca, E., Núñez, S., de la Herran, R., Navas, J. I., Manchado, M., . . . Toranzo, A. E. (2009). Identification of Vibrio harveyi isolated from diseased cultured wedge sole Dicologoglossa cuneata. Diseases of Aquatic Organisms, 84, 209-217. doi:10.3354/dao02045
  • Pujalte, M., Sitja-Bobadilla, A., Macián, M., Belloch, C., Alvarez-Pellitero, P., Perez-Sanchez, J., . . . Garay, E. (2003). Virulence and molecular typing of Vibrio harveyi strains isolated from cultured dentex, gilthead sea bream and European sea bass. Systematic and applied microbiology, 26(2), 284-292. doi:10.1078/072320203322346146
  • Qin, Y., Wang, J., Su, Y., Wang, D., & Chen, X. (2006). Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara. Acta Oceanologica Sinica, 25(1).
  • Russell, B., Pollard, D., Mann, B.Q., Buxton, C.D. & Carpenter, K.E. (2014). Sarpa salpa. The IUCN Red List of Threatened Species. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.2014-3.RLTS.T170169A1286510.en.
  • Saeed, M. (1995). Association of Vibrio harveyi with mortalities in cultured marine fish in Kuwait. Aquaculture, 136(1), 21-29. doi:10.1016/0044-8486(95)01045-9
  • Suau, A., Bonnet, R., Sutren, M., Godon, J.-J., Gibson, G. R., Collins, M. D., & Doré, J. (1999). Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Applied and environmental microbiology, 65(11), 4799-4807.
  • Tlusty, M. F., Rhyne, A. L., Kaufman, L., Hutchins, M., Reid, G. M., Andrews, C., . . . Dowd, S. (2013). Opportunities for public aquariums to increase the sustainability of the aquatic animal trade. Zoo Biology, 32(1), 1-12. doi:10.1002/zoo.21019
  • Turgay, E., & Karataş, S. (2016). First Report of Vibrio harveyi Infection in Diseased Common Dentex (Dentex dentex) Cultured in Turkey. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 12(2), 170-176.
  • Whitman, K. A. (2004). Finfish and Shellfish Bacteriology Manual: Techniques and Procedures: Iowa state press.
  • Zhang, Z., Schwartz, S., Wagner, L., & Miller, W. (2000). A greedy algorithm for aligning DNA sequences. Journal of Computational biology, 7(1-2), 203-214. doi:10.1089/10665270050081478
  • Zorrilla, I., Arijo, S., Chabrillon, M., Diaz, P., Martinez‐Manzanares, E., Balebona, M., & Morinigo, M. (2003). Vibrio species isolated from diseased farmed sole, Solea senegalensis (Kaup), and evaluation of the potential virulence role of their extracellular products. Journal of fish diseases, 26(2), 103-108. doi:10.1046/j.1365-2761.2003.00437.x
There are 20 citations in total.

Details

Primary Language English
Subjects Hydrobiology
Journal Section Articles
Authors

Emre Turgay

Remziye Eda Yardımcı

Süheyla Karataş

Publication Date September 1, 2018
Published in Issue Year 2018 Volume: 14 Issue: 3

Cite

APA Turgay, E., Yardımcı, R. E., & Karataş, S. (2018). First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 14(3), 166-172. https://doi.org/10.22392/egirdir.371581
AMA Turgay E, Yardımcı RE, Karataş S. First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium. JEFF. September 2018;14(3):166-172. doi:10.22392/egirdir.371581
Chicago Turgay, Emre, Remziye Eda Yardımcı, and Süheyla Karataş. “First Case of Vibriosis Caused by Vibrio Harveyi in Salema (Sarpa Salpa) from a Public Aquarium”. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi 14, no. 3 (September 2018): 166-72. https://doi.org/10.22392/egirdir.371581.
EndNote Turgay E, Yardımcı RE, Karataş S (September 1, 2018) First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi 14 3 166–172.
IEEE E. Turgay, R. E. Yardımcı, and S. Karataş, “First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium”, JEFF, vol. 14, no. 3, pp. 166–172, 2018, doi: 10.22392/egirdir.371581.
ISNAD Turgay, Emre et al. “First Case of Vibriosis Caused by Vibrio Harveyi in Salema (Sarpa Salpa) from a Public Aquarium”. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi 14/3 (September 2018), 166-172. https://doi.org/10.22392/egirdir.371581.
JAMA Turgay E, Yardımcı RE, Karataş S. First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium. JEFF. 2018;14:166–172.
MLA Turgay, Emre et al. “First Case of Vibriosis Caused by Vibrio Harveyi in Salema (Sarpa Salpa) from a Public Aquarium”. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, vol. 14, no. 3, 2018, pp. 166-72, doi:10.22392/egirdir.371581.
Vancouver Turgay E, Yardımcı RE, Karataş S. First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium. JEFF. 2018;14(3):166-72.