Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa acuminata × Musa balbisiana) 'Cardava'

Ronel V. Maximo

Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa acuminata × Musa balbisiana) 'Cardava'

Abstract

Under in vitro plant culture, light is the essential environmental cue that affects plant photosynthesis and photomorphogenesis. Fluorescent and high-pressure sodium lamps are common sources of light. Nevertheless, the wide spectral distribution of these traditional lights leads to the ineffective availability of some spectral qualities linked to photomorphogenesis. As a consideration, this study was conducted to test the effectivity of red-light emitting diode in the shoot tip culture of banana. The experiment was arranged in (CRD) Completely Randomized Design and replicated 3 times with 3 treatments in each replication. The treatment 1 – Control (Natural Lighting), T2 – Red Light Emitting Diode (LED) at 8 of hours exposure, and T3 - Red LED at 24 hours of exposure. The colored lighting especially the Red LED greatly influenced the in vitro culture of banana. The results show that the explant exposed to red-light at 24 hours obtained the highest final biomass, increment of initial and final biomass, lowest percent contamination, lowest tissue browning, and highest percent tissue greening of the shoot tip culture of banana. However, the natural lighting obtained the lightest weight of final biomass weight, initial and final increment, highest tissue browning, lowest percentage of tissue greening, and highest contamination.

Keywords

References

Bantis F., Ouzounis T. & Radoglou K. 2021. Artificial LED lighting enhances plant photosynthesis and biomass accumulation. Plants,10(3), 526. Doi: 10.3390/plants10030526
Batista D.S., Felipe S.H.S., Silva T.D., de Castro K.M., Mamedes-Rodrigues T.C., Miranda N.A., Ríos-Ríos A.M., Faria D.V., Fortini E.A. & Chagas K. 2018. Light quality in plant tissue culture: Does it matter? Vitr. Cell. Dev. Biol. Plant.; 54, 195-215. Doi: 10.1007/s11627-018-9902-5.
Cavallaro V., Arena C., Giuffrida F. & Toscano S. 2023. Effects of spectral quality and light quantity of LEDs on in vitro plant growth and morphogenesis. Agronomy, 13(4), 1072. Doi: 10.3390/agronomy13041072
Chen M., Chory J. & Fankhauser C. 2004. Light signal transduction in higher plants. Annu. Rev. Genet. 38, 87-117. Doi: 10.1146/annurev.genet.38.072902.092259.
Cioć M., Szewczyk A., Żupnik M., Kalisz A. & Pawłowska B. 2017. LED lighting affects plant growth, morphogenesis and phytochemical contents of Myrtus communis L. in vitro. Plant Cell, Tissue and Organ Culture (PCTOC), Doi:10.1007/s11240-017-1340-2
Edmale U.V., Huang S.C., Zander M., Cole B.J., Hetzel J., Ljung K. & Chory J. 2016. Cryptochromes interact directly with PIFs to control plant growth in limiting blue light. Cell. 164, 233–245
He C., Davies F. T. & Lacey R. E. 2020. Light quality affects in vitro plant growth and morphogenesis: Physiological and biochemical mechanisms. PeerJ, 8, e9180. Doi: 10.7717/peerj.9180
Holderbaum D. F., Kon T., Kudo T. & Guerra, M. P. 2010. Enzymatic Browning, Polyphenol Oxidase Activity, and Polyphenols in Four Apple Cultivars: Dynamics during Fruit Development. HortScience horts, 45(8), 1150-1154. Retrieved Sep 21, 2024, doi: 10.21273/HORTSCI.45.8.1150

Johkan M. K., Shoji F., Goto S., Hahida N. & Yoshihara T. 2012. Effect of green light wavelength and intensity on photomorphogenesis and photosynthesis in Lactuca sativa, Environmental and Experimental Botany, 75, 128-133.
Kapoor S., Raghuvanshi R., Bhardwaj P., Sood H., Saxena S. &. Chaurasia O.P. 2018. Influence of light quality on growth, secondary metabolites production and antioxidant activity in callus culture of Rhodiola imbricata Edgew, Journal of Photochemistry and Photobiology B: Biology, 183, 258–265.
Köhler K.H., Dörfler M. & Göring H. 1980. The influence of light on the cytokinin content of Amaranthus seedlings, Biologia Plantarum, 22(2), 128-134.
Kozai T. 2016. Why LED lighting for urban agriculture? In: Kozai T., Fujiwara K., Runkle E., editors. LED Lighting for Urban Agriculture. Springer; Singapore.
Li C.X., Xu Z.G., Dong R.Q., Chang S.X., Wang L.Z., Khalil-Ur-Rehman M. & Tao J.M. 2017. An RNA-Seq analysis of grape plantlets grown in vitro reveals different responses to blue, green, red led light, and white fluorescent light. Front. Plant Sci.
Muneer S., Kim E.J. & Park J.S. 2014. Influence of green, red and blue light emitting diodes on multiprotein complex proteins and photosynthetic activity under different light intensities in lettuce leaves (Lactuca sativa L.) Int. J. Mol. Sci. 15, 4657-4670. Doi: 10.3390/ijms15034657.
Park Y. & Runkle E. S. 2021. Far-red radiation promotes growth of seedlings by increasing leaf expansion and photosynthetic capacity. Horticulture Research, 8(1), 1-11. Doi: 10.1038/s41438-021-00529-4
Pawłowska B., Żupnik M., Szewczyk-Taranek B. & Cioć M. 2022. Impact of LED light spectra and photoperiod on plant growth, morphogenesis, and photoreceptor activity in in vitro cultures. Plants, 11(4), 529. Doi: 10.3390/plants11040529
Permadi N., Nurzaman M., Alhasnawi A. N., Doni F., & Julaeha E. 2023. Managing Lethal Browning and Microbial Contamination in Musa spp. Tissue Culture: Synthesis and Perspectives. Horticulturae. MDPI. Doi: 10.3390/horticulturae9040453
Poothong, S., Sirikantaramas, S., & Mekprasert, C. 2023. Light quality regulates hormone signaling and shoot proliferation during in vitro plant propagation. Plant Cell, Tissue and Organ Culture, 153(2), 237-250. Doi: 10.1007/s11240-023-02465-3
Shirani S., Mahdavi F. & Maziah M. 2009. Morphological abnormality among regenerated shootsof banana and plantain (Musa spp.) after in vitro multiplication with TDZ and BAP from excised shoot tips. Afr. J. Biotechnol. 8(21), 5755-5761.
Stefanova M., et al. 2024. LED light spectrum affects biomass accumulation and stomatal characteristics of in vitro propagated plants. Journal of Central European Agriculture, 25(1), 45–58.
Vendrame W. A., Moore K. K. & Carvalho, V. S. 2022. In vitro growth responses of ornamental bananas (Musa spp.) as affected by light sources. Horticulturae, 8(2), 92. Doi: 10.3390/horticulturae8020092
Yu L., Song C., Sun L., LI L., XU Z. & Tang C. 2020. Effects of light-emitting diodes on tissue culture plantlets and seedlings of rice (Oryza sativa L.). Journal of Integrative Agriculture, 19(7), 1743-1754. doi:10.1016/S2095-3119(19)62793-0

Details

Primary Language

English

Subjects

Sustainable Agricultural Development

Journal Section

Research Article

Authors

Ronel V. Maximo ^* This is me
Philippines

Publication Date

December 31, 2025

Submission Date

September 7, 2025

Acceptance Date

December 18, 2025

Published in Issue

Year 2025 Volume: 9 Number: 2

IZ

https://izlik.org/JA53HN27XX

Cite

RIS / Bibtex

APA

Maximo, R. V. (2025). Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa acuminata × Musa balbisiana) ’Cardava’. Eurasian Journal of Agricultural Research, 9(2), 179-190. https://izlik.org/JA53HN27XX

AMA

1.Maximo RV. Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa acuminata × Musa balbisiana) ’Cardava’. EJAR. 2025;9(2):179-190. https://izlik.org/JA53HN27XX

Chicago

Maximo, Ronel V. 2025. “Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa Acuminata × Musa Balbisiana) ’Cardava’”. Eurasian Journal of Agricultural Research 9 (2): 179-90. https://izlik.org/JA53HN27XX.

EndNote

Maximo RV (December 1, 2025) Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa acuminata × Musa balbisiana) ’Cardava’. Eurasian Journal of Agricultural Research 9 2 179–190.

IEEE

[1]R. V. Maximo, “Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa acuminata × Musa balbisiana) ’Cardava’”, EJAR, vol. 9, no. 2, pp. 179–190, Dec. 2025, [Online]. Available: https://izlik.org/JA53HN27XX

ISNAD

Maximo, Ronel V. “Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa Acuminata × Musa Balbisiana) ’Cardava’”. Eurasian Journal of Agricultural Research 9/2 (December 1, 2025): 179-190. https://izlik.org/JA53HN27XX.

JAMA

1.Maximo RV. Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa acuminata × Musa balbisiana) ’Cardava’. EJAR. 2025;9:179–190.

MLA

Maximo, Ronel V. “Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa Acuminata × Musa Balbisiana) ’Cardava’”. Eurasian Journal of Agricultural Research, vol. 9, no. 2, Dec. 2025, pp. 179-90, https://izlik.org/JA53HN27XX.

Vancouver

1.Ronel V. Maximo. Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa acuminata × Musa balbisiana) ’Cardava’. EJAR [Internet]. 2025 Dec. 1;9(2):179-90. Available from: https://izlik.org/JA53HN27XX