Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa acuminata × Musa balbisiana) 'Cardava'

Year 2025, Volume: 9 Issue: 2, 179 - 190, 31.12.2025

Ronel V. Maximo

https://izlik.org/JA53HN27XX

Abstract

Under in vitro plant culture, light is the essential environmental cue that affects plant photosynthesis and photomorphogenesis. Fluorescent and high-pressure sodium lamps are common sources of light. Nevertheless, the wide spectral distribution of these traditional lights leads to the ineffective availability of some spectral qualities linked to photomorphogenesis. As a consideration, this study was conducted to test the effectivity of red-light emitting diode in the shoot tip culture of banana. The experiment was arranged in (CRD) Completely Randomized Design and replicated 3 times with 3 treatments in each replication. The treatment 1 – Control (Natural Lighting), T2 – Red Light Emitting Diode (LED) at 8 of hours exposure, and T3 - Red LED at 24 hours of exposure. The colored lighting especially the Red LED greatly influenced the in vitro culture of banana. The results show that the explant exposed to red-light at 24 hours obtained the highest final biomass, increment of initial and final biomass, lowest percent contamination, lowest tissue browning, and highest percent tissue greening of the shoot tip culture of banana. However, the natural lighting obtained the lightest weight of final biomass weight, initial and final increment, highest tissue browning, lowest percentage of tissue greening, and highest contamination.

Keywords

Banana , In vitro culture , Red-light emitting diode , Natural lighting , Shoot tip culture

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