Determination of Effective Mutagen Dose for Lettuce (Lactuca sativa var. longifolia cv. Cervantes) Seeds

Abstract

In plant breeding strategies induced mutagenesis has become an effective way of supplementing the existing germplasm and improving new varieties. Mutation can be induced by chemical and physical mutagens or by combination. The commercial importance and production of lettuce, which is the most popular of the local salad crops, is increasing in Turkey. Lettuce is an important vegetable commodity and in demand by the local markets throughout the year. This study was carried out to get database for mutation breeding studies on lettuce cv. Cervantes. For this aim, 0, 50, 100, 200, 300, 400, 500 and 600 Gray (Gy) doses of Co⁶⁰ (gamma-rays) were treated on lettuce seeds as a physical mutagen. 30 seeds were used for each dose. Thirty days after treatment, germination and shoot developing of cloves were determined. The Effective Mutagen Dose (EMD₅₀) calculated by linear regression analyses. According to results, 372.66 Gy dose was found as EMD₅₀.

Keywords

• Lettuce,mutation breeding,Co60,gamma ray,EMD50,Lactuca sativa

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