Effect of vitamin B3 supplementation on glutathione redox cycle

Abstract

In this study, the effect of vitamin B3 supplement given to rats was investigated on glutathione redox cycle by looking at glutathione peroxidase and glutathione s transferase activities. 20 Wistar albino male rats were used. Vitamin B3 supplement was given to one of the two groups that were formed. The other group was determined as the control group. 360 mg/kg/day vitamin B3 supplement was given by oral gavage method for 10 days. At the end of 10 days, intracardiac blood samples were taken. Glutathione peroxidase activity level was determined as 1033.44±198.05 U/L in the vitamin B3 supplement group and 526.00±99.54 U/L in the control group. The glutathione peroxidase activity level of the vitamin B3 supplemented group was found to be statistically significantly higher than the glutathione peroxidase activity level of the control group (p<0.001, t=7.239). This difference has huge impact size (d=3.24). Glutathione s transferase activity level was determined as 7.50±0.67 U/mL in the vitamin B3 supplement group and 6.50±0.97 U/mL in the control group. The glutathione s transferase activity level of the group given vitamin B3 supplementation was found to be statistically significantly lower than the glutathione s transferase activity level of the control group (p=0.015, t=2.698). This difference has a very large effect size (d=1.20). As a result, vitamin B3 supplementation led to an increase in glutathione peroxidase activity at a huge effect size and a decrease in glutathione s transferase activity at a very large effect size.

Keywords

• Vitamin B3
• Glutathione peroxidase
• Glutathione s transferase
• Glutathione redox cycle

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