pH controlled release of doxorubicin from Fe3O4@-CRG-graft-PDMAEMA magnetic nanoparticles

Abstract

In recent years, the effectiveness of magnetically biocompatible nanoparticles in cancer treatment has been increased. Targeted drug delivery system has recently emerged as a promising cancer treatment because it reduces the side effects of traditional cancer treatment methods and increases the effectiveness of treatment. In our study, hybrid magnetic nanoparticles were synthesized. Nanoparticles consist of an inorganic iron oxide core (Fe3O4) and an organic copolymer (-CRG-graft-PDMAEMA). The magnetic nanoparticles’ structure was characterized by UV and Zeta-sizer techniques. Fe3O4@-CRG-graft-PDMAEMA@DOX magnetic nanoparticles were obtained by loading the anti-tumor cancer drug doxorubicin (DOX) on the synthesized Fe3O4@-CRG-graft-PDMAEMA nanoparticles. The in vitro release of drug-loaded magnetic nanoparticles in phosphate buffer (pH 7.4), acetate buffer (pH 5.5) and acidic medium (pH 1.2) at 37 °C was investigated. It has been shown that the synthesized Fe3O4@-CRG-graft-PDMAEMA@DOX magnetic nanoparticles are pH sensitive and have high release performance. The DOX release of Fe3O4@-CRG-graft-PDMAEMA@DOX nanoparticles in pH 7.4, pH 5.5 and pH 1.2 mediums was found to be 66.53%, 70.08% and 90.47% s, respectively. Kinetic calculations of magnetic nanoparticles were made. The iron content of magnetic nanoparticles was found to be 66.77%.

Keywords

• Manyetik nanopartikül
• doksorubisin
• kopolimer
• demir oksit

Fe3O4@-KRG-aşı-PDMAEMA manyetik nanopartiküllerden pH kontrollü doksorubisin salımı

Abstract

Son yıllarda manyetik yapılı biyouyumlu nanoparçacıkların kanser tedavisinde etkinliği artmaktadır. Hedefli ilaç salım sistemi, geleneksel kanser tedavi yöntemlerinin yan etkilerini azaltmakta ve tedavi etkinliğini artırması sebebiyle yakın zamanda umut verici kanser tedavisi olarak ortaya çıkmaktadır. Çalışmamızda hibrit yapılı manyetik nanopartiküller sentezlenmiştir. Nanopartiküller anorganik yapılı demir oksit çekirdeği (Fe3O4) ve organik yapılı kopolimerden (-KRG-aşı-PDMAEMA) oluşmaktadır. Manyetik nanopartiküllerin yapısı UV ve Zeta-sizer ile karakterize edilmiştir. Sentezlenen Fe3O4@-KRG-aşı-PDMAEMA nanopartiküllerine anti-tümör etkiye sahip kanser ilacı doksorubisin (DOX) yüklenerek Fe3O4@-KRG-aşı-PDMAEMA@DOX manyetik nanopartikülleri elde edilmiştir. İlaç yüklü manyetik nanopartiküllerin fosfat tamponunda (pH 7,4), asetat tamponunda (pH 5,5) ve asidik ortamda (pH 1,2) 37 oC’de in vitro salımı incelenmiştir. Sentezlenen Fe3O4@-KRG-aşı-PDMAEMA@DOX manyetik nanopartiküllerin pH’ya duyarlı olduğu ve yüksek salım performansına sahip olduğu gösterildi. Fe3O4@-KRG-aşı-PDMAEMA@DOX nanopartiküllerin DOX salımı pH 7,4, pH 5,5 ve pH 1,2 ortamlarında sırası ile %66,53, %70,08 ve %90,47 bulunmuştur. Manyetik nanopartiküllerin kinetik hesaplamaları yapılmıştır. Manyetik nanopartiküllerin demir içeriği %66,77 bulunmuştur.

Keywords

• Manyetik nanopartikül
• doksorubisin
• kopolimer
• demir oksit

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