Drosophila melanogaster’in somatik hücrelerinde kobalt nanopartiküllerinin indüklediği genotoksisiteye karşı resveratrol’ün antigenotoksik etkisi

Year 2022, , 50 - 55, 28.12.2022

Ayşen Yağmur Kurşun , Burcin Yalcin , Merve Güneş , Ghada Tagorti , Bülent Kaya

https://doi.org/10.46239/ejbcs.1069388

Cited By: 1

Abstract

Son yıllarda gelişen teknoloji ile birlikte yeni teknolojik ürün olarak nanopartikül (NP,<100 nm) üretimi ve farklı alanlardaki kullanımları hemen her alanda giderek yaygınlaşmıştır. NP’lerin çok farklı alanlarda birçok avantajı nedeniyle yaygın olarak kullanılmalarına karşın insan sağlığına ve çevreye etkileri hakkındaki bilgiler hala çok yetersizdir. Bu nedenle son teknoloji ürünü bu maddelerin genotoksik potansiyellerinin tespiti bu maddelerin biyogüvenilirliği bakımından önemlidir. Resveratrol (3, 4', 5-trihidroksi-stilben: RSV) üzüm çekirdeğinde bol miktarda bulunan ve son yıllarda farklı mekanizmalardaki yardımcı etkileri üzerinde yoğun çalışılan doğal bir antioksidandır. Bu çalışmada, Drosophila melanogaster’de somatik mutasyon ve rekombinasyon testi (SMART) ve tek hücre alkali jel elektroforezi testi (KOMET) kullanılarak Kobalt nanopartiküllerinin genotoksisitesine karşı Resveratrol’ün antigenotoksik etkisi araştırılmıştır. SMART genetik değişimleri geniş bir spektrumda hızlı ve ucuz şekilde belirlemeye yarayan güvenilir bir yöntemdir. KOMET ise özgün hücrelerde değişen alkali lezyonları ve DNA’daki tek iplik kırıklarını tespit eden farklı genotoksik ajanlar ile indüklenen genetik hasarın belirlenmesinde güçlü ve duyarlı bir tekniktir. Yapmış olduğumuz çalışma kapsamında SMART ve KOMET yöntemleri ile değerlendirilen ve somatik hücreler olan kanat imajinal disk hücrelerinde ve Drosophila hemositlerinde genotoksik etkiye sahip olduğunu tespit ettiğimiz Kobalt nanopartiküllerinin (Co NP, 10mM) Resveratrol ile hem ön uygulamalı hem de eş zamanlı çalışmalarında Co NP’ün genotoksik etkisine karşı RSV’ün uygulanan tüm dozlarda (0.1, 0.5 ve 2.5 mM) antigenotoksik etkiye sahip olduğu gözlenmiştir.

Keywords

Drosophila melanogaster, Kobalt nanopartikül, Resveratrol, Antigenotoksisite, SMART, COMET

Supporting Institution

TÜBİTAK

Project Number

113Z564

Thanks

Bu çalışma TÜBİTAK tarafından 1002 Hızlı Destek Programı kapsamında (Proje No: 113Z564) desteklenmiştir.

Antigenotoxic effect of resveratrol against genotoxicity induced by cobalt nanoparticles in somatic cells of Drosophila melanogaster

Abstract

With the developing technology in recent years, the production of nanoparticles (NP, <100 nm) as a new technological product and their use in different fields have become increasingly widespread in almost every field. Although NPs are widely used in many different fields due to their many advantages, the information about their effects on human health and the environment is still very insufficient. Therefore, the determination of the genotoxic potential of these state-of-the-art substances is important for their biosafety. Resveratrol (3, 4', 5-trihydroxy-stilbene: RSV) is a natural antioxidant that is abundant in grape seeds and its auxiliary effects in different mechanisms have been studied intensively in recent years. The antigenotoxic effect of Resveratrol against genotoxicity of Cobalt Nanoparticles was investigated using single cell alkaline gel electrophoresis test (KOMET). SMART is a reliable method for quickly and inexpensively detecting a wide spectrum of genetic changes. KOMET, on the other hand, is a powerful and sensitive technique for the detection of genetic damage induced by different genotoxic agents that detect altered alkaline lesions and single strand breaks in DNA in specific cells. Within the scope of our study, Cobalt Nanoparticles (Co NP, 10mM) which were evaluated by SMART and KOMET methods and we found to have genotoxic effects in wing imaginal disc cells which are somatic cells and Drosophila hemocytes, were able to resist the genotoxic effect of Co NP in both pre-application and simultaneous studies with Resveratrol. It was observed that RSV had an antigenotoxic effect at all doses (0.1, 0.5 and 2.5 mM).

Keywords

Drosophila melanogaster, Cobalt nanoparticle, Resveratrol, Antigenotoxicity, SMART, COMET

Project Number

113Z564

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