Peroxidase Mimicking Activity of Macroporous Carbon

Abstract

In this study, the peroxidase-like activity of macroporous carbon manufactured using a silica template was investigated. The nanozyme activity of macroporous carbon was compared to commercial graphene oxide. The field emission scanning electron microscopy image of carbon revealed macroporous morphology. The nanozyme activity was studied via the catalytic oxidation of chromogenic substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) in the presence of hydrogen peroxide and the oxidized form of ABTS with a green color can be visualized by the eyes. Without functionalization and enzyme utilization, the fabricated macroporous carbon demonstrated green color development, indicating its peroxidase activity probably due to the large surface area and, thus, abundant active sites present on the surface. The oxygen-containing functional groups formed during carbonization act as active sites and can play a pivotal role in the peroxidase-mimicking activity.

Keywords

• Nanozyme
• Carbonaceous material
• ABTS
• Peroxidase-like activity
• Sustainability

Makro gözenekli karbonun peroksidaz mimik aktivitesi

Abstract

Bu çalışmada, silika şablonu kullanılarak üretilen makro gözenekli karbonun peroksidaz mimik aktivitesi araştırılmıştır. Makro gözenekli karbonun nanozim aktivitesi, ticari grafen oksit ile karşılaştırıldı. Karbonun alan emisyon taramalı elektron mikroskobu görüntüsü, makro gözenekli morfolojiyi ortaya çıkardı. Nanozim aktivitesi, kromojenik substrat 2,2'-azino-bis(3-etilbenzotiazolin-6-sülfonik asidin (ABTS) hidrojen peroksit varlığında katalitik oksidasyonu yoluyla incelenmiştir ve yeşil renkli ABTS'nin oksitlenmiş formu gözle görülebilecek şekilde oluştu. İşlevselleştirme ve enzim kullanımı olmadan, elde edilen makro gözenekli karbon yeşil renk gelişimi gösterdi, bu da muhtemelen geniş yüzey alanı ve dolayısıyla yüzeyde bulunan bol miktarda aktif bölge nedeniyle peroksidaz aktivitesini gösteriyor. Karbonizasyon sırasında oluşan oksijen içeren fonksiyonel gruplar, aktif bölgeler olarak davranabilir ve peroksidazı taklit eden aktivitede çok önemli bir rol oynayabilir.

Keywords

• Nanozim
• Karbonlu malzeme
• ABTS
• Peroksidaz mimik aktivite
• Sürdürülebilirlik

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