Moringa oleifera bitki yaprağının mineral ve yağ asidi bileşenlerinin belirlenmesi

Abstract

Son yıllarda bitkilerin besinsel bileşenleri, sağlık üzerindeki etkileri nedeniyle pek çok çalışmalara konu olmuştur. Bu çalışmada Moringa oleifera yaprak tozunda mineral ve yağ asidi içeriği belirlenmiştir. Element analizi için numuneler, bir mikrodalga fırında HNO3 ve HCl ile sindirildi. Numunelerde mineral konsantrasyonları İndüktif Eşleşmiş Plazma Kütle Spektrometresi (ICP-MS) ile belirlendi. Numunelerde magnezyum 6393 μg g-1, demir 927 μg g-1, çinko 138 μg g-1 ve bakır ise 6 μg g-1 olarak tespit edilmiştir. Yağ asiti ekstraksiyonu sonrası, yağ asidi ve ester profilleri için Gaz Kromatografisi Alev İyonlaşmalı Dedektörü (GC-FID) kullanılarak tanımlanmıştır. Moringa oleifera yaprağında yağ asitlerinin ana bileşenleri olarak lauric asit (C12:0), tridekanoik asit (C13:0), miristik asit (C14:0), palmitik asit (C16:0), stearik asit (C18:0), oleik asit (C18:1), linoleik asit (C18:2), gama-linolenik asit (C18:3) tayin edilmiştir.

Keywords

• Moringa oleifera
• mineral tayini
• yağ asidi analizi

Moringa oleifera bitki yaprağının mineral ve yağ asidi bileşenlerinin belirlenmesi

Abstract

In recent years, the nutritional components of plants have been the subject of many studies due to their effects on health. In this study, mineral and fatty acid content of Moringa oleifera leaf powder was determined. Samples were digested with HNO3 and HCl in a microwave oven for element analysis. In samples, mineral concentrations were determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The mineral content was determined as magnesium 6393 μg g-1, iron 927 μg g-1, zinc 138 μg g-1, and cupper 6 μg g-1 by Inductively Coupled Plasma Mass Spectrometry in samples. After fatty acid extraction, fatty acid and ester profiles were verified using Gas Chromatography Flame Ionization Detection (GC-FID). The main components of fatty acids in Moringa oleifera leaf were determined as lauric acid (C12:0), tridecanoic acid (C13:0), myristic acid (C14:0), palmitic acid (C16:0), stearic acid (C18:0), oleic acid. (C18:1), linoleic acid (C18:2), gamma-linolenic acid (C18:3).

Keywords

• Moringa oleifera
• mineral determination
• fatty acid analysis

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