The potential use of Epilobium hirsutum L. in phytoremediation of zinc and an efficient method for in vitro propagation

Abstract

This study aimed to evaluate the capacity of Epilobium hirsutum L. (Onagraceae), a wetland plant, to accumulate and tolerate zinc (Zn) and its in vitro propagation potential. Root-shoot length, fresh weight, pigment, and protein content were analyzed in the plants grown in different Zn concentrations, including 0, 10, 20, 30, 40, 50, 75, 100,150, and 200 mg Zn/L. In the seedlings grown at 50 and 75 mg Zn/L concentrations, a reduction in the relative root length, shoot length, and fresh weight was detected. It was found that there was a negative correlation between pigment and protein contents of E. hirsutum and increased Zn concentrations of solutions. On the other hand, it was determined that a considerable amount of Zn was accumulated by E. hirsutum in its roots (10 598 mg Zn/kg DW). In tissue culture experiments, it was found that MS medium was effective for the germination of the plant (97%). When the growth parameters of plants grown in different concentrations of Gibberellic acid were evaluated, the highest growth parameters were obtained at 50 mg/L. It has been concluded that the most successful mediums on shoot development were 1.0BAP/1.0NAA and 1.0BAP/1.0IBA. The highest number of shoots per explant was 1.0BAP/1.0NAA (3.96). The longest root length was also determined on medium with 1.0BAP/1.0IBA (0.28 cm). Regenerated shoots were transferred to different concentrations of root mediums. It was concluded that MS medium with 1.0IBA has been superior for root formation compared to other hormone concentrations.

Keywords

• Phytoremediation
• Purple loosestrife
• Zinc

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