Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes

Abstract

Bacopa monnieri L. Pennell is an important medicinal plant with antioxidant and neuroprotective role. It acts as an efficient free radical scavenger and has the potential to serve as a phytoremedy in the disease conditions involving reactive oxygen species (ROS) and oxidative stress. In addition, the protective role of B. monnieri on tissue antioxidant defense system and lipid peroxidative status in diabetic rats has been reported. B. monnneri used in the experiment was propagated by plant tissue culture techniques. The shoot tip explants of B. monnneri were cultured in Murashige and Skoog (MS) nutrient medium containing 1.0 mg/L 6-Benzylaminopurine (BAP). Genotoxic effects of aqueous extract obtained from B. monnieri in cultured human lymphocytes were determined by chromosome aberration (CA) test. In addition, cytotoxic activities of the extract on the cells were revealed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis. Different concentrations (6.25-200 mg/L) of the extract were used in the treatments. It was detected that cytotoxicty was correlated with concentration. Cell viability decreased to 69.64% in the lymphocytes exposed to maximum concentration of the extract. Based on CA/cell test, the results were statistically (p > 0.05) indifferent from negative control treatment. Overall, obtained data indicated that B. monnieri is a plant that contains natural products that are not causative of cytotoxic and genotoxic damage on human lymphocytes.

Keywords

• Cytotoxicity,Genotoxicity,Shoot Regeneration

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