Exopolysaccharide (EPS) production of halophilic bacteria

Abstract

Exopolysaccharides (EPS), which are produced by halophilic bacteria and have protective properties against adverse environmental conditions, have wide application areas. In this study, EPS production of Halomonas aquamarina (NB2) and Halobacillus trueperi (NB7, NB8, NB9, NB10, NB11) strains in culture media was determined. EPS production of the strains was range from 15-55 mg/L. One strain with the highest EPS production capacity and the best development in the culture medium (NB7-47 mg/L) was selected and the influence of different concentrations of salt (5, 10, 15, 17.5, 20, 25, and 30%) and different carbon sources (sucrose, glucose, galactose, mannose) on EPS production was studied. When NB7 strain was grown in medium containing different concentrations of salt, the EPS production was changed (0-40 mg/L). The highest level of EPS production of the NB7 strain was observed in the medium with sucrose (488 mg/L) while the lowest level of EPS production of the NB7 strain was observed in the medium with galactose (144 mg/L). In this study, also, biofilm activities of these strains was determined. NB2, NB2, NB7, NB8, NB9 and NB10 strains were found to be strong biofilm producers and NB11 strains were intermediate biofilm producers. Finally, emulsification of lyophilized EPS (1-EPS) were designated. Emulsification activity of l-EPSs on hydrocarbons was found in the range of 3-26%. In the field of environment application of lyophilized EPS obtained from halophilic bacteria used in this study; it has the potential for use as an emulsifying agent.

Keywords

• Halophilic bacteria,exopolysaccharide,emulsification,biofilm

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