Research Article
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Year 2020, , 6 - 9, 22.06.2020
https://doi.org/10.46239/ejbcs.602904

Abstract

Project Number

KAMAG-110G007

References

  • Atta-ur-Rahman, Nasım S, Baig I, Sener B, Orhan I, Ayanoğlu F, Choudhary MI 2004. Two new isoflavonoids from the rhizomes of Iris soforana, Natural Product Research, 18(5): 465-471.
  • Bhat G, Shawl AS, Shah Z, Tantry M 2014. HPLC-DAD-ESI-MS/MS Identification and characterization of major constituents of Iris crocea, Iris germanica and Iris spuria L. growing in Kashmir Himalayas, India. Journal of Analytical and Bioanalytical Analyses: 5(6), 1-10.
  • Ellman GL, Courtney KD, Andres V, Featherstone RM 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2): 88-95.
  • Güner A, 2012. Iris L. In: Güner A, Aslan S, Vural M, Babaş MT (Eds.), Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul: Nezahat Gökyiğit Botanic Garden and Floristic Research Society, pp. 535-540.
  • Howes MJR, Houghton PJ, Perry NSL 2003. Plants with traditional uses and activities, relevant to the management of Alzheimer’s disease and other cognitive disorders. Phytotheraphy Research, 17: 1-18.
  • Orhan I, Nasim S, Şener B, Ayanoğlu F, Özgüven M, Choudhary MI, Atta-ur-Rahman 2003. Two isoflavones and bioactivity spectrum of the crude extracts of Iris germanica rhizomes. Phytotherapy Research, 17: 575-577.
  • Schütz C, Quitschau M, Hamburger M, Potterat O 2011. Profiling of isoflavonoids in Iris germanica rhizome extracts by microprobe NMR and HPLC-PDA-MS analysis. Fitoterapia, 82: 1021-1026.
  • Sevim D, Senol FS, Gulpinar AR, Erdogan Orhan I, Kaya E, Kartal M, Sener B 2013. Discovery of potent in vitro neuroprotective effect of the seed extracts from seven Paeonia L. (peony) taxa and their fatty acid composition. Industrial Crops and Products, 49: 240-246.
  • Sevim D 2018. Pharmacognosic studies on some Iris L. species. Ph. D. Thesis, Ankara: Gazi University Institute of Health Sciences, pp. 1-219.
  • Singab ANB, Ayoub IM, El-Shazyl M, Korinek M, Wu T, Cheng Y, Chang F, Wu Y 2016. Shedding the light on Iridaceae - Ethnobotany, phytochemistry and biological activity. Industrial Crops and Products, 92: 308-335.
  • Şener B, Orhan İ 2003. Alzheimer hastalığının tedavisinde geliştirilen bileşikler. İlaç ve Tedavi Dergisi, 16(1): 3-8.
  • Xie G, Zhu Y, Shu P, Qin X, Wu G, Wang Q, Qin M 2014. Phenolic metabolite profiles and antioxidants assay of three Iridaceae medicinal plants for traditional Chinese medicine ‘She-gan’ by on-line HPLC-DAD coupled with chemiluminescence (CL) and ESI-Q-TOF-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 98: 40-51.
  • Wang H, Cui Y, Zhao C 2010. Flavonoids of the genus Iris (Iridaceae). Mini-Reviews in Medicinal Chemistry, 10: 643-661.

Isolation and Identification of Cholinesterase Inhibitors from the Bulbs of Iris pseudacorus L.

Year 2020, , 6 - 9, 22.06.2020
https://doi.org/10.46239/ejbcs.602904

Abstract

Most of the bulbous
plants are known for their medicinal purposes in addition to their ornamental
value. Turkey is one of the home country of many beautiful bulbous plants. In
continuation of our extensive studies on finding new natural cholinesterase inhibitors
from Turkish medicinal plants, Iris
L. species were investigated for their in
vitro
cholinesterase inhibitory effects
designed to
assess cholinesterase inhibitor activities on both acetylcholinesterase (AChE)
and butyrylcholinesterase (BChE)
and antioxidant
capacities with respect to their neuroprotective potential in this study.



The dichloromethane and
methanol extracts prepared from the bulbs of 47 Iris taxa were screened by using modified Ellmann method and the
highest butyrylcholinesterase inhibitory effect was found in the methanol
extract of the bulbs of Iris pseudacorus
L. The dichloromethane sub-extract, which is obtained bioactivity-guided
fractionation of methanol extract of I.
pseudacorus
L., was exhibited significant butyrylcholinesterase inhibitory
activity (73.65 ± 2.06 %).
These active sub-extract was subjected to fractionation on column
chromatography and obtained six fractions. Among the fractions, coded as N5 was shown the significant butyrylcholinesterase
inhibitory activity (93.78 ±
1.49 %) compared with
galanthamine (80.02 ± 0.12 %). Fractionation of N5 on flash chromatography the
highest butyrylcholinesterase inhibitory activity of sub-fraction coded as DS-5 was determined as 94.00
± 1.03 %. The responsible compound from the activity of this
sub-fraction was detected as irisolidone glucopyranoside based on its mass data
by using LC-ESI-Q/TOF-MS-MS technique.

Supporting Institution

TÜBİTAK

Project Number

KAMAG-110G007

Thanks

Turkish Scientific and Technological Research Council (TÜBİTAK) for financial source (Project code: KAMAG-110G007) was kindly acknowledged.

References

  • Atta-ur-Rahman, Nasım S, Baig I, Sener B, Orhan I, Ayanoğlu F, Choudhary MI 2004. Two new isoflavonoids from the rhizomes of Iris soforana, Natural Product Research, 18(5): 465-471.
  • Bhat G, Shawl AS, Shah Z, Tantry M 2014. HPLC-DAD-ESI-MS/MS Identification and characterization of major constituents of Iris crocea, Iris germanica and Iris spuria L. growing in Kashmir Himalayas, India. Journal of Analytical and Bioanalytical Analyses: 5(6), 1-10.
  • Ellman GL, Courtney KD, Andres V, Featherstone RM 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2): 88-95.
  • Güner A, 2012. Iris L. In: Güner A, Aslan S, Vural M, Babaş MT (Eds.), Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul: Nezahat Gökyiğit Botanic Garden and Floristic Research Society, pp. 535-540.
  • Howes MJR, Houghton PJ, Perry NSL 2003. Plants with traditional uses and activities, relevant to the management of Alzheimer’s disease and other cognitive disorders. Phytotheraphy Research, 17: 1-18.
  • Orhan I, Nasim S, Şener B, Ayanoğlu F, Özgüven M, Choudhary MI, Atta-ur-Rahman 2003. Two isoflavones and bioactivity spectrum of the crude extracts of Iris germanica rhizomes. Phytotherapy Research, 17: 575-577.
  • Schütz C, Quitschau M, Hamburger M, Potterat O 2011. Profiling of isoflavonoids in Iris germanica rhizome extracts by microprobe NMR and HPLC-PDA-MS analysis. Fitoterapia, 82: 1021-1026.
  • Sevim D, Senol FS, Gulpinar AR, Erdogan Orhan I, Kaya E, Kartal M, Sener B 2013. Discovery of potent in vitro neuroprotective effect of the seed extracts from seven Paeonia L. (peony) taxa and their fatty acid composition. Industrial Crops and Products, 49: 240-246.
  • Sevim D 2018. Pharmacognosic studies on some Iris L. species. Ph. D. Thesis, Ankara: Gazi University Institute of Health Sciences, pp. 1-219.
  • Singab ANB, Ayoub IM, El-Shazyl M, Korinek M, Wu T, Cheng Y, Chang F, Wu Y 2016. Shedding the light on Iridaceae - Ethnobotany, phytochemistry and biological activity. Industrial Crops and Products, 92: 308-335.
  • Şener B, Orhan İ 2003. Alzheimer hastalığının tedavisinde geliştirilen bileşikler. İlaç ve Tedavi Dergisi, 16(1): 3-8.
  • Xie G, Zhu Y, Shu P, Qin X, Wu G, Wang Q, Qin M 2014. Phenolic metabolite profiles and antioxidants assay of three Iridaceae medicinal plants for traditional Chinese medicine ‘She-gan’ by on-line HPLC-DAD coupled with chemiluminescence (CL) and ESI-Q-TOF-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 98: 40-51.
  • Wang H, Cui Y, Zhao C 2010. Flavonoids of the genus Iris (Iridaceae). Mini-Reviews in Medicinal Chemistry, 10: 643-661.
There are 13 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences
Journal Section Research Articles
Authors

Duygu Sevim 0000-0003-3987-2466

Bilge Şener This is me 0000-0002-7672-3489

Project Number KAMAG-110G007
Publication Date June 22, 2020
Acceptance Date February 2, 2020
Published in Issue Year 2020

Cite

APA Sevim, D., & Şener, B. (2020). Isolation and Identification of Cholinesterase Inhibitors from the Bulbs of Iris pseudacorus L. Eurasian Journal of Biological and Chemical Sciences, 3(1), 6-9. https://doi.org/10.46239/ejbcs.602904
AMA Sevim D, Şener B. Isolation and Identification of Cholinesterase Inhibitors from the Bulbs of Iris pseudacorus L. Eurasian J. Bio. Chem. Sci. June 2020;3(1):6-9. doi:10.46239/ejbcs.602904
Chicago Sevim, Duygu, and Bilge Şener. “Isolation and Identification of Cholinesterase Inhibitors from the Bulbs of Iris Pseudacorus L”. Eurasian Journal of Biological and Chemical Sciences 3, no. 1 (June 2020): 6-9. https://doi.org/10.46239/ejbcs.602904.
EndNote Sevim D, Şener B (June 1, 2020) Isolation and Identification of Cholinesterase Inhibitors from the Bulbs of Iris pseudacorus L. Eurasian Journal of Biological and Chemical Sciences 3 1 6–9.
IEEE D. Sevim and B. Şener, “Isolation and Identification of Cholinesterase Inhibitors from the Bulbs of Iris pseudacorus L”., Eurasian J. Bio. Chem. Sci., vol. 3, no. 1, pp. 6–9, 2020, doi: 10.46239/ejbcs.602904.
ISNAD Sevim, Duygu - Şener, Bilge. “Isolation and Identification of Cholinesterase Inhibitors from the Bulbs of Iris Pseudacorus L”. Eurasian Journal of Biological and Chemical Sciences 3/1 (June 2020), 6-9. https://doi.org/10.46239/ejbcs.602904.
JAMA Sevim D, Şener B. Isolation and Identification of Cholinesterase Inhibitors from the Bulbs of Iris pseudacorus L. Eurasian J. Bio. Chem. Sci. 2020;3:6–9.
MLA Sevim, Duygu and Bilge Şener. “Isolation and Identification of Cholinesterase Inhibitors from the Bulbs of Iris Pseudacorus L”. Eurasian Journal of Biological and Chemical Sciences, vol. 3, no. 1, 2020, pp. 6-9, doi:10.46239/ejbcs.602904.
Vancouver Sevim D, Şener B. Isolation and Identification of Cholinesterase Inhibitors from the Bulbs of Iris pseudacorus L. Eurasian J. Bio. Chem. Sci. 2020;3(1):6-9.