Isolation and Identification of Cholinesterase Inhibitors from the Bulbs of Iris pseudacorus L.

Year 2020, , 6 - 9, 22.06.2020

Duygu Sevim , Bilge Şener

https://doi.org/10.46239/ejbcs.602904

Cited By: 1

Abstract

Most of the bulbous
plants are known for their medicinal purposes in addition to their ornamental
value. Turkey is one of the home country of many beautiful bulbous plants. In
continuation of our extensive studies on finding new natural cholinesterase inhibitors
from Turkish medicinal plants, Iris
L. species were investigated for their in
vitro cholinesterase inhibitory effects designed to
assess cholinesterase inhibitor activities on both acetylcholinesterase (AChE)
and butyrylcholinesterase (BChE) and antioxidant
capacities with respect to their neuroprotective potential in this study.

The dichloromethane and
methanol extracts prepared from the bulbs of 47 Iris taxa were screened by using modified Ellmann method and the
highest butyrylcholinesterase inhibitory effect was found in the methanol
extract of the bulbs of Iris pseudacorus
L. The dichloromethane sub-extract, which is obtained bioactivity-guided
fractionation of methanol extract of I.
pseudacorus L., was exhibited significant butyrylcholinesterase inhibitory
activity (73.65 ± 2.06 %).
These active sub-extract was subjected to fractionation on column
chromatography and obtained six fractions. Among the fractions, coded as N5 was shown the significant butyrylcholinesterase
inhibitory activity (93.78 ±
1.49 %) compared with
galanthamine (80.02 ± 0.12 %). Fractionation of N5 on flash chromatography the
highest butyrylcholinesterase inhibitory activity of sub-fraction coded as DS-5 was determined as 94.00
± 1.03 %. The responsible compound from the activity of this
sub-fraction was detected as irisolidone glucopyranoside based on its mass data
by using LC-ESI-Q/TOF-MS-MS technique.

Keywords

Iris pseudacorus L., Iridaceae, Anticholinesterase, Activity

Supporting Institution

TÜBİTAK

Project Number

KAMAG-110G007

Thanks

Turkish Scientific and Technological Research Council (TÜBİTAK) for financial source (Project code: KAMAG-110G007) was kindly acknowledged.

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