Multiple shoot induction and plant regeneration of Staurogyne repens (Nees) Kuntze

Abstract

Staurogyne repens (Nees) Kuntze is an aquatic plant belonging to Acanthaceae family. It is a valuable plant in the aquarium industry. Therefore, there has been a significant demand for this plant. This study was designed for multiple and efficient productions of S. repens under in vitro conditions. He used nodal meristems as the type of explant. Nodal meristems were preferred as explant type. The explants were transferred to Murashige and Skoog (MS) food media with TDZ single (0-1.50 mg/L) and TDZ (0.25-1.50 mg/L) + IAA (0.25 mg/L) combinations. In TDZ application, the most number of shoots per explant (15.36 shoots/explants) was recorded in cultures with 1.50 mg/L TDZ, while in TDZ + IAA application, the most number of shoots (9.44 shoots/explants) was determined in culture with 1.0 mg/L TDZ + 0.25 mg/L IAA. In general, shoot lengths in TDZ + IAA combination was measured higher than the single application of TDZ. The longest shoot (1.67 cm) was obtained in the culture medium with 1.0 mg/L TDZ + 0.25 mg/L IAA. The extended shoots were transposed to rooting media with 0.25 mg/L IAA, and multiple root formations were determined after four weeks. The rooted plants were transferred to the aquarium and successfully accustomed to ex vitro conditions.

Keywords

• Acclimatization,propagation,shoot regeneration,tissue culture

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