Production of indole-3acetic acid (IAA) using Erwinia herbicola with vitreoscilla hemoglobin gene transferred

Abstract

Indole-3-acetic acid (IAA) is an important natural auxin hormone found in plants. IAA biosynthesis is a common condition among plant-related bacteria and there are different biosynthetic ways to produce this hormone. In this study was investigated the production of IAA in E. herbicola carrying the Vitreoscilla hemoglobin (VHb) gene. The effect of endogenous recombinant oxygen intake system on the production of IAA was studied for the first time. The production of IAA in the recombinant strain carrying the VHb gene (vgb +) in rich and poor medium was noted to be higher than in the wild and vgb- control strains. The recombinant strains had significantly higher oxygen uptake than the host. The highest IAA level was observed in the stationary phase cultures rich in L-tryptophan.

Keywords

• Erwinia herbicola
• vitreoscilla hemoglobini
• Indol-3-asetik asit (IAA)

Production of indole-3acetic acid (IAA) using Erwinia herbicola with vitreoscilla hemoglobin gene transferred

Abstract

İndol-3-asetik asit (IAA) bitkilerde bulunan önemli bir doğal oksin hormonudur. IAA biyosentezi, bitki ile ilişkili bakteriler arasında yaygın bulunan bir durum olup, bu hormonun üretimi için farklı biyosentetik yollar bulunmaktadır. Bu çalışmada, Vitreoscilla hemoglobinin (VHb) geni taşıyan rekombinat Erwinia herbicola’da IAA üretimi araştırılmıştır. E. herbicola’da bu şekilde endojen rekombinant bir oksijen alım sisteminin IAA üzerindeki etkisi ilk defa çalışılmıştır. VHb geni (vgb+) taşıyan rekombinant suşun zengin ve minimal besiyerlerinde ürettiği IAA seviyelerinin yabanıl ve vgb- kontrol suşularından daha yüksek olduğu kaydedildi. Rekombinant suşların konakçıdan önemli derecede yüksek oksijen alımına sahip oldukları belirlendi. En yüksek IAA’nın seviyesi geç kütür fazında L- triptofan içeren kültürlerde saptandı.

Keywords

• Erwinia herbicola
• Vitreoscilla hemoglobini
• Indol-3-asetik asit (IAA)
• rekombinant DNA teknolojisi

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