Effects of Aluminum Oxide on Total Hemocyte Counts of Galleria mellonella (L.) (Lepidoptera: Pyralidae) Larvae

Abstract

The heavy metals are one of the important factors of environmental pollution. Heavy metal pollution can cause adverse effect not only on growth, reproduction, survival, and metabolism in animals, but also effects the innate immune system adversely. Different concentrations of Al2O3 solution (10, 50 and 100 µg/mL) were injected into the proleg of the last instar Galleria mellonella larvae by Hamilton injector for 2, 4 and 8 hours. Then, the total hemocyte count was determined with the hemolymph obtained from the control and experimental groups. As a result of the data obtained, G. mellonella larvae exposed to Al2O3 solution showed a decrease in total hemocyte count in 2, 4 and 8 hours in all concentrations compared to the control and this reduction was found to be statistically significant. Changes in hemocyte numbers of G. mellonella larvae have shown that it can be used as a good model organism in evaluating environmental pollution levels and it can also lead to immunological and physiological studies on other species.

Keywords

• galleria mellonella
• Aluminum Oxide
• Total Hemocyte Count

Alüminyum oksit’in Galleria mellonella (L.) (Lepidoptera: Pyralidae) Larvalarında Total Hemosit Sayıları Üzerine Etkileri

Abstract

Ağır metaller, çevre kirliliğinin önemli etkenlerinden biridir. Ağır metal kirliliği sadece hayvanlarda büyüme, üreme, hayatta kalma ve metabolizma ile değil aynı zamanda doğuştan gelen bağışıklık sisteminde de zararlı etkilere neden olabilir. Yapılan çalışmada, Galleria mellonella son evre larvalarının birinci arka bacaklarına Al2O3 çözeltisinin farklı derişimleri (10, 50 ve 100 µg/mL) Hamilton enjektörü yardımıyla enjekte edilerek 2, 4 ve 8 saatlik sürelere maruz bırakılmıştır. Uygulama sonunda kontrol ve deneme gruplarından alınan hemolenf örnekleri ile total hemosit sayısı belirlenmiştir. Elde edilen veriler sonucunda, Al2O3 çözeltisine maruz bırakılan G. mellonella larvalarının maruz bırakılan etki sürelerinde total hemosit sayısında kontrole göre tüm konsantrasyonlarda azalma meydana gelmiş ve bu azalma istatistiki açıdan önemli bulunmuştur. G.mellonella larvalarının total hemosit sayılarında meydana gelen bu değişimler, çevre kirliliği düzeylerini değerlendirmede iyi bir model organizma olarak kullanılabilirliğini ve başka türler üzerinde de yapılacak immünolojik ve fizyolojik çalışmalara yol gösterebileceğini ortaya koymuştur.

Keywords

• Galleria mellonella
• Alüminyum oksit
• Total Hemosit Sayısı

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