Antioxidant properties of Melissa officinalis L. callus cultures

Year 2018, Volume: 1 Issue: 1, 12 - 15, 15.08.2018

Aykut Topdemir , Nazmi Gür , Zümre Demir

Abstract

Melissa
officinalis L. is a Mediterranean plant from the family of the
Labitae, and has lemon fragrance. Melissa is a perennial herbaceous plant that
forms the plant size between 60-100 cm. It is a known and used plant for a long
time, because of its pleasant smell. Because it is effective in beekeeping,
this plant is called as the swarm plant. It is a medicinal and aromatic plant,
since it contains essential oils containing citral, stranellal, linalool and
pinemia and flavonoid and resin. The antioxidant activity of the in vitro
produced calli was determined in this study, which was also determined by
previous studies. Melissa officinalis
L. nodals were used as an explant source. Nodules were promoted with different
plant growth regulator combinations for callus formation in Murashige Skoog
medium. The antioxidant activity of the resulting callus was determined by the
ABTS method and the results were given as "TEAC (tropoxyl equivalent
antioxidant capacity) equivalent". The highest antioxidant capacity (0.365
mmol / L) was seen in 1.5 mg / L 2,4-D + 1 mg / L PIC + 0.5 mg / L KIN-induced
callus. The lowest value (0.191 mmol / L) was obtained from calli promoted with
1.5 mg / L 2,4-D + 0.5 mg / L BAP.

Keywords

Melissa officinalis L, callus, antioxidant

Melissa officinalis L. kallus kültürlerinin antoksidan özellikleri

Abstract

Melissa officinalis L., Labitae familyasından,
Akdeniz bitkisi olup  limon kokuludur.
Melisa 60-100 cm arasında bitki boyu oluşturan çok yıllık otsu bir bitkidir.
Hoş kokusu nedeniyle uzun zamandır bilinen ve kullanılan bir bitkidir.
Arıcılıkta oğul ekmede etkili olduğu için, halk arasında bu bitkiye oğul otu da
denilmektedir. Sitral,stranellal,linalol ve pinemi içeren uçucu yağlar ile
flavonoid ve reçine içerdiğinden dolayı tıbbi ve aromatik bir bitkidir.
Çalışmamızda antoksidan özelliği olduğu da daha önce yapılan çalışmalarla
belirlenmiş olan bu bitkiden, in vitro olarak üretilen kallusların antioksidan
aktivitesi belirlenmiştir. Explant kaynağı olarak Melissa officinalis L. nodları kullanılmıştır. Nodlar Murashige
Skoog besiyerinde kallus oluşumu için farklı bitki büyüme düzenleyici
kombinasyonları ile teşvik edilmiştir. Sonuçta oluşan kallusların antioksidan
aktivitesi ABTS yöntemi ile belirlenmiş ve sonuçlar “TEAC (troloks eşdeğer
antioksidan kapasite) eşdeğeri” olarak verilmiştir. En yüksek antioksidan
kapasite (0.365 mmol/L), 1.5 mg/L 2,4-D + 1 mg/L PİC + 0.5 mg/L KİN ile
indüklenmiş kalluslarda görülmüştür. En düşük değer ise (0.191 mmol/L), 1.5
mg/L 2,4-D + 0.5 mg/L BAP ile teşvik edilmiş kalluslardan elde edilmiştir.

Keywords

Melissa officinalis L, kallus, antioksidan.

References

• Allahverdiyev, A., Duran, N., Ozguven, M. and Koltas, S. 2004. Antiviral activity of the volatile oils of Melissa officinalis L. against Herpes simplex virus type-2. Phytomedicine, 11(7-8), 657-661.
• Argyropoulos, D., Müller, J. 2014. Changes of essential oil content and composition during convective drying of lemon balm (Melissa officinalis L.). Industrial Crops and Products. 52. 118-224.
• Awad, R., Muhammad, A., Durst, T., Trudeau, V. T., Arnason, J. T. 2009. Bioassay‐guided fractionation of lemon balm (Melissa officinalis L.) using an in vitro measure of GABA transaminase activity. Phytothrapy Research, Volume23, Issue8, 1075-1081.
• Bahri-Sahloul, R., Ben Fredj, R., Boughalleb, N., Shriaa, J., Saguem, S., Hilbert, J. L. and Harzallah-Skhiri, F. 2014. Phenolic composition and antioxidant and antimicrobial activities of extracts obtained from Crataegus azarolus L. var. aronia (Willd.) Batt. ovaries calli. Journal of Botany, 2014.
• Basta, A., Tzakou, O. and Couladis, M. 2005. Composition of the leaves essential oil of Melissa officinalis sl from Greece. Flavour and fragrance journal, 20(6), 642-644.
• Farahani, H. A., Valadabadi, S. A., Daneshian, J. and Khalvati, M. A. 2009. Evaluation changing of essential oil of balm (Melissa officinalis L.) under water deficit stress conditions. Journal of Medicinal Plants Research, 3(5), 329-333.
• Giri, L., Dhyani, P., Rawat, S., Bhatt, I. D., Nandi, S. K., Rawal, R. S. and Pande, V. 2012. In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenaria edgeworthii: a rare Himalayan medicinal orchid. Industrial Crops and Products, 39, 1-6.
• Grzegorczyk, I., Matkowski, A. and Wysokinska, H. 2007. Antioxidant activity of extracts from in vitro cultures of Salvia officinalis L. Food Chemistry, 104(2), 536-541.
• Kousalya, L. and Bai, V. N. 2016. Effect of growth regulators on rapid micropropagation and antioxidant activity of Canscora decussata (Roxb.) Roem. & Schult.–A threatened medicinal plant. Asian Pacific Journal of Reproduction, 5(2), 161-170.
• Moradkhani, H., Sargsyan, E., Bibak, H., Naseri, B., Sadat-Hosseini, M., Fayazi-Barjin, A. and Meftahizade, H. 2010. Melissa officinalis L., a valuable medicine plant. Journal of Medicinal Plants Research Vol. 4(25), pp. 2753-2759.
• Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3), 473-497.
• Saglam, C., Atakisi, I., Turhan, H., Kaba, S., Arslanoglu, F. and Onemli, F. 2004. Effect of propagation method, plant density, and age on lemon balm (Melissa officinalis) herb and oil yield.
• Sarı, A. O. and Ceylan, A. 2002. Yield characteristics and essential oil composition of lemon balm (Melissa officinalis L.) grown in the Aegean region of Turkey. Turkish Journal of Agriculture and Forestry, 26(4), 217-224.
• Simon, J. E. and Chadwick, A. F. 1984. Herbs an indexed bibliography 1971-1980: the scientific literature on selected herbs and aromatic and medicinal plants of the temperature zone, Archon Books.
• Yesil-Celiktas, O., Nartop, P., Gurel, A., Bedir, E. and Vardar-Sukan, F. 2007. Determination of phenolic content and antioxidant activity of extracts obtained from Rosmarinus officinalis’ calli. Journal of plant physiology, 164(11), 1536-1542.
• Zeybek, U. and Haksel, M. 2010. Important medicinal plants and their use in Turkey and around the world. Argefar and Helvacızade Medical Publications, Izmir.