Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes

Year 2018, Volume: 1 Issue: 2, 48 - 53, 20.12.2018

Muhammet Dogan , Bugrahan Emsen

Abstract

Bacopa
monnieri L. Pennell is an
important medicinal plant with antioxidant and neuroprotective role. It acts as an efficient free radical scavenger and
has the potential to serve as a phytoremedy in the disease conditions involving
reactive oxygen species (ROS) and oxidative stress. In
addition, the protective role of B. monnieri on
tissue antioxidant defense system and lipid peroxidative status in diabetic
rats has been reported. B. monnneri
used in the experiment was propagated by plant tissue culture techniques. The
shoot tip explants of B. monnneri
were cultured in Murashige and Skoog (MS) nutrient medium containing 1.0 mg/L
6-Benzylaminopurine (BAP). Genotoxic effects of aqueous extract
obtained from B. monnieri in
cultured human lymphocytes were determined by
chromosome aberration (CA) test. In addition, cytotoxic activities of the
extract on the cells were revealed via
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis.
Different concentrations (6.25-200 mg/L) of the extract were used in the
treatments. It was detected that cytotoxicty was correlated with concentration.
Cell viability decreased to 69.64% in the lymphocytes exposed to maximum
concentration of the extract. Based on CA/cell test, the results were
statistically (p > 0.05)
indifferent from negative control treatment. Overall, obtained data indicated
that B. monnieri is a plant that
contains natural products that are not causative of cytotoxic and genotoxic
damage on human lymphocytes.

Keywords

Cytotoxicity, Genotoxicity, Shoot Regeneration

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