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Farklı ışık yayan diyotlar (LED) altında tıbbi sucul bitki Lysimachia nummularia L.'nın boğum eksplantlarından çoklu sürgün rejenerasyonu

Year 2019, Volume: 2 Issue: 1, 11 - 16, 20.07.2019

Abstract

Işık yayan diyotlar (LED) bitki büyüme ve gelişimi için
önemli avantajlara sahip olduklarından dolayı doku kültürü çalışmalarında
kullanılmaya başlanmıştır. LED’lerin ışık renkleri bitkiler üzerinde farklı
etkiler göstermektedir. Bu çalışmada, beyaz (B), kirmizi (K), mavi (M) LED
ışıkların L. nummularia'nın çoklu
sürgün rejenerasyonu üzerine tek ve kombine etkileri incelenmiştir. Boğum
eksplantları 0,10 mg/L indol butirik asit (IBA) ve 0,20-0,80 mg/L Thidiazuron
(TDZ) eklenmiş Murashige ve Skoog (MS) besin ortamında sekiz hafta süre ile
kültürlenmiştir. Her üç hormon uygulamasında %100 sürgün rejenerasyon
frekansları 2B:1K:1M ve 1B:1K:1M LED ışıkları altında kaydedilmiştir. En fazla
sürgün sayısı 1B:2K:1M LED ışıklar altında sırasıyla 17,47, 16,16 ve 13,46 adet
olarak sırasıyla 0,40 mg/L TDZ + 0,10 mg/L IBA, 0,20 mg/L TDZ + 0,10 mg/L IBA
ve 0,80 mg/L TDZ + 0,10 mg/L IBA eklenmiş MS besin ortamında belirlenmiştir. En
az sayıda sürgünler ise mavi LED altında kaydedilmiştir. En uzun sürgünler tüm
ortamlarda 1B:1K:2M LED ışık altındaki 
eksplantlardan elde edilmiştir. TDZ’nin kültür ortamında fazla
kullanılması sürgün uzunluğunu olumsuz etkilemiştir. Çoğaltım denemelerinde
yoğun kök oluştuğundan dolayı ayrıca köklendirme yapılmamıştır. Kültür
ortamında üretilen bitkiler dış koşullara başarıyla alıştırılmıştır. Sonuç
olarak çalışma, LED ışıkların L.
nummularia
’nın üretimindeki etkinliğini ve flouresan ışıklara göre
üstünlüğünü göstermiştir.




Supporting Institution

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)

Project Number

2130190

Thanks

Bu çalışma, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) tarafından desteklenmiştir (Proje no:2130190).

References

  • Acemi A, Bayrak B, Çakır M, Demiryürek E, Gün E, El Gueddari NE, Özen F 2018. Comparative analysis of the effects of chitosan and common plant growth regulators on in vitro propagation of Ipomoea purpurea (L.) Roth from nodal explants. In Vitro Cell Dev Biol Plant 54(5): 537-544.
  • Bourget CM 2008. An introduction to light-emitting diodes. HortScience 43(7): 1944-1946.
  • Dănilă E, Lucache DD 2013. Cost effectiveness of growing plant lighting system. Journal of Electrical Engineering 13(4): 224-229.
  • Debnath SC 2005. Micropropagation of lingonberry: influence of genotype, explant orientation, and overcoming TDZ-induced inhibition of shoot elongation using zeatin. HortScience 40(1): 185-188.
  • Dogan M 2018a. In vitro shoot regeneration of Limnophila aromatica (Lamk.) Merr. from nodal and internodal explants. Iğdır Univ J Inst Sci & Tech 8: 77-84.
  • Dogan M 2019a. Multiple shoot regeneration via indirect organogenesis from shoot tip and nodal meristem explants of Ceratophyllum demersum L. J Anim Plant Sci 29: 568-577.
  • Dogan M 2019b. Callus formation from full leaf and leaf parts of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Acta Biologica Turcica 32: 78-83.
  • Dogan M 2019c. In vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze. Ant J Bot 3: 1-6.
  • Dogan M 2018b. In vitro micropropagation from nodal explants of the medicinal plant Lysimachia nummularia L.. KSU J Agric Nat 21(6): 875-881.
  • Dogan M, Emsen B 2018. Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes. Eurasian J Bio Chem Sci 1(2): 48-53.
  • Dougher T, Bugbee B 2001. Differences in the response of wheat, soybean and lettuce to reduced blue radiation. Photochem Photobiol 73: 199- 207.
  • Emsen B, Dogan M 2018. Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts. Acta Sci Pol-Hortoru 17: 23-33.
  • Fatonah S, Isda MN 2018. In vitro shoot regeneration of tembesu (Fagraea fragrans Roxb.) from seed explants on different concentrations of sucrose and honey. Biosci Res 15(2): 655-662.
  • Gupta SD, Sahoo TK 2015. Light emitting diode (LED)-induced alteration of oxidative events during in vitro shoot organogenesis of Curculigo orchioides Gaertn. Acta Physiol Plant 37(11): 233.
  • Heo J, Lee C, Chakrabarty D, Paek K 2002. Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED). Plant Growth Regulat 38: 225-230.
  • Ledbetter DI, Preece JE 200). Thidiazuron stimulates adventitious shoot production from Hydrangea quercifolia Bartr. leaf explants. Sci Hort 101(1-2): 121-126.
  • Lin KH, Huang MY, Huang WD, Hsu MH, Yang ZW, Yang CM. 2013. The effects of red, blue, and white light-emitting diodes on the growth, development, and edible quality of hydroponically grown lettuce (Lactuca sativa L. var. capitata), Sci Hort 150: 86-91.
  • Lu YX, Godo T, Fujiwara K, Guan KY, Mii M 2013. Effects of nitrogen source and wavelength of led-light on organogenesis from leaf and shoot tip cultures in Lysionotus pauciflorus maxim. Propag Ornam Plants 13: 174-180.
  • Morrow RC 2008. LED lighting in horticulture. HortScience 43(7): 1947-1950.
  • Murashige T, Skoog F 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.
  • Najar RA, Fayaz M, Bhat MH, Bashir M, Kumar A, Jain AK 2018. An efficient micropropagation protocol for direct organogenesis from nodal explants of medicinal climber, Tylophora indica. Biosci Biotech Res Comm 11(1): 144-153.
  • Rameshkumar R, Largia MJV, Satish L, Shilpha J, Ramesh M 2017. In vitro mass propagation and conservation of Nilgirianthus ciliatus through nodal explants: A globally endangered, high trade medicinal plant of Western Ghats. Plant Biosyst 151(2): 204-211.
  • Sharma U, Agrawal V 2018. In vitro shoot regeneration and enhanced synthesis of plumbagin in root callus of Plumbago zeylanica L.-an important medicinal herb. In Vitro Cell Dev Biol Plant 54(4): 423-435.
  • Snedecor GW, Cochran WG 1997.Statistical methods. Iowa, USA: The Iowa State University Press, USA.
  • Tomsone S, Gertnere D, Novikova D 2004. The influence of thidiazuron on shoot regeneration and proliferation of rhododendrons in vitro. Acta Univ Latv 676: 239-242.
  • Venkatachalam P, Jinu U, Gomathi M, Mahendran D, Ahmad N, Geetha N, Sahi SV 2017. Role of silver nitrate in plant regeneration from cotyledonary nodal segment explants of Prosopis cineraria (L.) Druce.: A recalcitrant medicinal leguminous tree. Biocatal Agric Biotechnol 12: 286-291.

Multiple shoot regeneration from nodal explants of medicinal aquatic plant Lysimachia nummularia L. under different light emitting diodes (LEDs)

Year 2019, Volume: 2 Issue: 1, 11 - 16, 20.07.2019

Abstract

Since light emitting diodes (LEDs) have important
advantages for plant growth and development, they have been used in tissue
culture studies. The light colours of LEDs show different effects on plants. In
this study, single and combined effects of white (W), red (R), blue (B) LED
lights on multiple shoot regeneration of Lysimachia
nummularia
L. were investigated. Nodal explants were cultured for eight
weeks in a Murashige and Skoog (MS) nutrient medium supplemented with 0.10 mg/L
indole butyric acid (IBA) and 0.20-0.80 mg/L Thidiazuron (TDZ). 100% shoot
regeneration frequencies in all three hormone applications were recorded under
2W:1R: 1B and 1W: 1R: 1B LED lights. The maximum number of shoots was
determined under 1W:2R:1B LED lights as 17.47, 16.16 and 13.46 shoots/explant
in the MS nutrient medium added 0.40 mg/L TDZ + 0.10 mg/L IBA, 0.20 mg/L TDZ +
0.10 mg/L and 0.80 mg/L TDZ + 0.10 mg/L IBA, respectively. The minimum number
of shoots was recorded under blue LED. The longest shoot was obtained from
explants under 1W:1R:2B LED light in all environments. Excessive use of TDZ in
the culture medium adversely affected shoot length. Since there was a dense
root in replication experiments, no rooting was performed. Plants produced in
the culture environment have been successfully adapted to external conditions.
As a result, the study showed the effectiveness of the LED lights on the
production of L. nummularia and their
superiority over the fluorescent lights.

Project Number

2130190

References

  • Acemi A, Bayrak B, Çakır M, Demiryürek E, Gün E, El Gueddari NE, Özen F 2018. Comparative analysis of the effects of chitosan and common plant growth regulators on in vitro propagation of Ipomoea purpurea (L.) Roth from nodal explants. In Vitro Cell Dev Biol Plant 54(5): 537-544.
  • Bourget CM 2008. An introduction to light-emitting diodes. HortScience 43(7): 1944-1946.
  • Dănilă E, Lucache DD 2013. Cost effectiveness of growing plant lighting system. Journal of Electrical Engineering 13(4): 224-229.
  • Debnath SC 2005. Micropropagation of lingonberry: influence of genotype, explant orientation, and overcoming TDZ-induced inhibition of shoot elongation using zeatin. HortScience 40(1): 185-188.
  • Dogan M 2018a. In vitro shoot regeneration of Limnophila aromatica (Lamk.) Merr. from nodal and internodal explants. Iğdır Univ J Inst Sci & Tech 8: 77-84.
  • Dogan M 2019a. Multiple shoot regeneration via indirect organogenesis from shoot tip and nodal meristem explants of Ceratophyllum demersum L. J Anim Plant Sci 29: 568-577.
  • Dogan M 2019b. Callus formation from full leaf and leaf parts of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Acta Biologica Turcica 32: 78-83.
  • Dogan M 2019c. In vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze. Ant J Bot 3: 1-6.
  • Dogan M 2018b. In vitro micropropagation from nodal explants of the medicinal plant Lysimachia nummularia L.. KSU J Agric Nat 21(6): 875-881.
  • Dogan M, Emsen B 2018. Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes. Eurasian J Bio Chem Sci 1(2): 48-53.
  • Dougher T, Bugbee B 2001. Differences in the response of wheat, soybean and lettuce to reduced blue radiation. Photochem Photobiol 73: 199- 207.
  • Emsen B, Dogan M 2018. Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts. Acta Sci Pol-Hortoru 17: 23-33.
  • Fatonah S, Isda MN 2018. In vitro shoot regeneration of tembesu (Fagraea fragrans Roxb.) from seed explants on different concentrations of sucrose and honey. Biosci Res 15(2): 655-662.
  • Gupta SD, Sahoo TK 2015. Light emitting diode (LED)-induced alteration of oxidative events during in vitro shoot organogenesis of Curculigo orchioides Gaertn. Acta Physiol Plant 37(11): 233.
  • Heo J, Lee C, Chakrabarty D, Paek K 2002. Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED). Plant Growth Regulat 38: 225-230.
  • Ledbetter DI, Preece JE 200). Thidiazuron stimulates adventitious shoot production from Hydrangea quercifolia Bartr. leaf explants. Sci Hort 101(1-2): 121-126.
  • Lin KH, Huang MY, Huang WD, Hsu MH, Yang ZW, Yang CM. 2013. The effects of red, blue, and white light-emitting diodes on the growth, development, and edible quality of hydroponically grown lettuce (Lactuca sativa L. var. capitata), Sci Hort 150: 86-91.
  • Lu YX, Godo T, Fujiwara K, Guan KY, Mii M 2013. Effects of nitrogen source and wavelength of led-light on organogenesis from leaf and shoot tip cultures in Lysionotus pauciflorus maxim. Propag Ornam Plants 13: 174-180.
  • Morrow RC 2008. LED lighting in horticulture. HortScience 43(7): 1947-1950.
  • Murashige T, Skoog F 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.
  • Najar RA, Fayaz M, Bhat MH, Bashir M, Kumar A, Jain AK 2018. An efficient micropropagation protocol for direct organogenesis from nodal explants of medicinal climber, Tylophora indica. Biosci Biotech Res Comm 11(1): 144-153.
  • Rameshkumar R, Largia MJV, Satish L, Shilpha J, Ramesh M 2017. In vitro mass propagation and conservation of Nilgirianthus ciliatus through nodal explants: A globally endangered, high trade medicinal plant of Western Ghats. Plant Biosyst 151(2): 204-211.
  • Sharma U, Agrawal V 2018. In vitro shoot regeneration and enhanced synthesis of plumbagin in root callus of Plumbago zeylanica L.-an important medicinal herb. In Vitro Cell Dev Biol Plant 54(4): 423-435.
  • Snedecor GW, Cochran WG 1997.Statistical methods. Iowa, USA: The Iowa State University Press, USA.
  • Tomsone S, Gertnere D, Novikova D 2004. The influence of thidiazuron on shoot regeneration and proliferation of rhododendrons in vitro. Acta Univ Latv 676: 239-242.
  • Venkatachalam P, Jinu U, Gomathi M, Mahendran D, Ahmad N, Geetha N, Sahi SV 2017. Role of silver nitrate in plant regeneration from cotyledonary nodal segment explants of Prosopis cineraria (L.) Druce.: A recalcitrant medicinal leguminous tree. Biocatal Agric Biotechnol 12: 286-291.
There are 26 citations in total.

Details

Primary Language Turkish
Subjects Structural Biology
Journal Section Research Articles
Authors

Muhammet Dogan 0000-0003-3138-5903

Project Number 2130190
Publication Date July 20, 2019
Acceptance Date June 5, 2019
Published in Issue Year 2019 Volume: 2 Issue: 1

Cite

APA Dogan, M. (2019). Farklı ışık yayan diyotlar (LED) altında tıbbi sucul bitki Lysimachia nummularia L.’nın boğum eksplantlarından çoklu sürgün rejenerasyonu. Eurasian Journal of Biological and Chemical Sciences, 2(1), 11-16.
AMA Dogan M. Farklı ışık yayan diyotlar (LED) altında tıbbi sucul bitki Lysimachia nummularia L.’nın boğum eksplantlarından çoklu sürgün rejenerasyonu. Eurasian J. Bio. Chem. Sci. July 2019;2(1):11-16.
Chicago Dogan, Muhammet. “Farklı ışık Yayan Diyotlar (LED) altında tıbbi Sucul Bitki Lysimachia Nummularia L.’nın boğum eksplantlarından çoklu sürgün Rejenerasyonu”. Eurasian Journal of Biological and Chemical Sciences 2, no. 1 (July 2019): 11-16.
EndNote Dogan M (July 1, 2019) Farklı ışık yayan diyotlar (LED) altında tıbbi sucul bitki Lysimachia nummularia L.’nın boğum eksplantlarından çoklu sürgün rejenerasyonu. Eurasian Journal of Biological and Chemical Sciences 2 1 11–16.
IEEE M. Dogan, “Farklı ışık yayan diyotlar (LED) altında tıbbi sucul bitki Lysimachia nummularia L.’nın boğum eksplantlarından çoklu sürgün rejenerasyonu”, Eurasian J. Bio. Chem. Sci., vol. 2, no. 1, pp. 11–16, 2019.
ISNAD Dogan, Muhammet. “Farklı ışık Yayan Diyotlar (LED) altında tıbbi Sucul Bitki Lysimachia Nummularia L.’nın boğum eksplantlarından çoklu sürgün Rejenerasyonu”. Eurasian Journal of Biological and Chemical Sciences 2/1 (July 2019), 11-16.
JAMA Dogan M. Farklı ışık yayan diyotlar (LED) altında tıbbi sucul bitki Lysimachia nummularia L.’nın boğum eksplantlarından çoklu sürgün rejenerasyonu. Eurasian J. Bio. Chem. Sci. 2019;2:11–16.
MLA Dogan, Muhammet. “Farklı ışık Yayan Diyotlar (LED) altında tıbbi Sucul Bitki Lysimachia Nummularia L.’nın boğum eksplantlarından çoklu sürgün Rejenerasyonu”. Eurasian Journal of Biological and Chemical Sciences, vol. 2, no. 1, 2019, pp. 11-16.
Vancouver Dogan M. Farklı ışık yayan diyotlar (LED) altında tıbbi sucul bitki Lysimachia nummularia L.’nın boğum eksplantlarından çoklu sürgün rejenerasyonu. Eurasian J. Bio. Chem. Sci. 2019;2(1):11-6.