The Effect of Repeated Doses of Ketamine on Hematological Parameters in Rats

Year 2019, Cilt 2 (Suppl 1), 102 - 106, 27.12.2019

Ufuk Mercan Yücel , Leyla Mis

Abstract

The aim of this study was to investigate the effect of
repeated doses of ketamine on hematological parameters in rats. Ketamine is a
dissociative anesthetic that general and rapid-acting phencyclidine derivative.
Ketamine, which is usually used in laboratory animals in combination with
xylazine, can be used alone for short-term anesthesia in rats. General
anesthesia applications repeated several times a day or on different days in
animal experiments are used in pharmacokinetic studies where blood or other samples
are required, in surgical procedures or drug applications on different days
under anesthesia, in treatment applications such as painful wound care, regular
dental care. In this study, 20 adult male Wistar albino rats, weighing between 180-230 g were used. Rats
were divided into two groups as 10 rats in each group. The rats in the ketamine
group received intraperitoneal injection of 10% Ketamine HCl at a dose of 50
mg/kg every other day for 12 days. The control group received 0.2 ml saline
intraperitoneally at the same time. At the end of the study, 2 ml blood
obtained by intracardiac route was placed in K₃EDTA tubes. Hematological parameters
include total leukocyte (WBC), erythrocyte
(RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean
corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH),
erythrocyte distribution width (RDW), lymphocyte (LYM), monocyte (MON), neutrophil (NEU),
lymphocyte%, monocyte%, neutrophil% levels were measured with autoanalyzer device.
According to the results, ketamine has statistically decreased only lymphocyte
count ​​(p <0.05). In conclusion, ketamine caused changes in the lymphocyte
values ​​according to the control group; however, since these changes were
within the range of reference values ​​reported for rats, it was concluded that
ketamine can be used safely in laboratory animals. Investigation of the effect
of the anesthetic agents on blood parameters in laboratory animals is important
in terms of the reliability of experimental studies.

Keywords

Ketamine, Rat

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