Farklı Besi Ortamlarında Yetişen Chlorella Vulgaris Türüne Ait Protein Ve Aminoasit Komposizyonunun Karşılaştırılması

Year 2020, Volume: 3 Issue: 2, 130 - 137, 29.12.2020

Merve Kavasbasıoglu İlhami Tuzun Yaşar Aluç

Abstract

Bu çalışmada, Kırıkkale Kapulukaya barajından izole edilen ve TAP (Tris-Acetate-Phosphate) besiyeri, Nitrat eklemeli TAP medium (N-TAP), Kırıkkale Üniversitesi kampüs gölü (GÖL), Makina ve Kimya Endüstrisi Kurumu (MKEK) atık suyu olmak üzere 4 farklı besi ortamında yetiştirilen mikroalg Chlorella vulgaris’in büyüme parametreleri (hücre sayımı, Optik Dansite ve Klorofil-a) takip edilmiştir. Durgun faza 15. günde ulaşan örneklerde biyokütle olarak en yüksek alg miktarı GÖL besiyerinde elde edilmiştir. Hasatı gerçekleştirilen alglerin toplam protein analizi Lowry metodu ile yapılmış ve protein miktarı sırasıyla; N-TAP (2,17 mg/BSA), TAP (1,96 mg/BSA), MKEK atık suyu (0,87 mg/BSA) ve GÖL besi ortamı (0,69 mg/BSA) olarak tespit edilmiştir. İyon Kromatografi kullanılarak yapılan besi ortamı anyon ölçümleri değerlendirildiğinde, MKEK ve GÖL besi ortamında yetişen Chlorella vulgaris türünde fosfat sınırlamasının diğer TAP ve N-TAP ortamlarına göre daha erken oluştuğu sonucuna varılmıştır. Fosfat sınırlamasının, N-TAP ve TAP besi ortamlarında yetişen alglerin protein içeriğinde artışa sebep olduğu FTIR analizleri ile belirlenmiştir. Amino asit ölçüm sonuçlarında tüm besi ortamlarında Arjinin amino asidi en yüksek olarak bulunmuştur. N-TAP ve TAP besi ortamlarında yetişen alglerde esansiyel ve esansiyel olmayan amino asit içerikleri bakımından yaklaşık 5 kat yüksek olduğu tespit edilmiştir.

Keywords

Mikroalg, Chlorella vulgaris, Amino asit, Protein, HPLC Analizi

Supporting Institution

Kırıkkale Üniversitesi Bilimsel Araştırmalar Proje Birimi

Project Number

2019/162

Comparison of total protein and amino acid compositions of microalgae Chlorella vulgaris grown in different growth media

Abstract

The microalgae species in this study, Chlorella vulgaris, isolated from Kapulukaya Reservoir (Kırıkkale), were subjected to four different growth media, being (i) Tris-Acetat-Phosphate (TAP), (ii) Tris-Acetat-Phosphate enriched with N (N-TAP), (iii) Kırıkkale University Campus Lake Water (LW) and (iv) Waste Water from Mechanical and Chemical Industry Company (MKEK) in order to compare its growth by monitoring the parameters, cell count (CC), Optical density (OD) and Chlorophyll-a (Chl-a). Of the experimental trials, all of which reached the stationary phase on day 15, the highest biomass was detected in the LW medium. Following the end of the experiments, amounts of total protein measured on the harvested biomass by using Lowry method were found to be 2,17 mg/BSA (N-TAP), 1,96 mg/BSA (TAP), 0,87 mg/BSA (MKEK) and 0,69 mg/BSA (LW). Results from anion measurements in the media suggested the idea that phosphate limitations occurred during growth but being onset earlier in the MKEK and LW media than that in the TAP and TAP-N media. The greater protein content found in TAP and TAP-N media corresponded with similarly higher values obtained from Fourier Transform Infrared Spectroscopy (FTIR) analysis. The amount of arginine was profoundly higher amongst amino acids. Both essential and non-essential amino acids were five-fold higher in N-TAP and TAP media compared to wastewater (MKEK) and LW. This suggested a general conclusion that the used natural waters were not compatible enough with the controlled media due mainly to that in natural waters phosphorus limited the growth despite efficient N levels.

Keywords

Mikroalg, Chlorella vulgaris, Amino asit, Protein, HPLC analizi

Project Number

2019/162

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