The Effect of “Tris-Hydroxymethyl Aminomethane” Treatment on Survival of Rats with Experimental Metabolic Acidosis Created by Intragastric Administration of Hydrochloric Acid

Year 2021, Volume: 3 Issue: 3, 98 - 104, 28.12.2021

Vehbi Özaydın , Gürkan Ersoy , Elvan Öçmen , Hanife Çiftçioğlu Yıldız , Osman Yılmaz , Necati Gökmen , Aslı Çelik , Kasım Öztürk

Abstract

Abstract
Background:
Treatment of acidosis is serious health problem within emergency departments and intensive care units etc. Ingestion of household cleaning solutions (due to both suicidal and accidental reasons) is still also a serious public health problem in Turkey and the most fatal complication thereof is, severe metabolic acidosis. An effective treatment should be provided for the cases presenting to emergency departments with life-threatening metabolic acidosis.
Aim:
We aimed to compare the survival rate of rats with experimental metabolic acidosis created by intragastric administration of hydrochloric acid and treated with “tris–hydroxymethyl aminomethane” or normal saline solution alone.
Study Design:
This was an experimental animal study
Methods:
Following ketamine-xylazine anesthesia, internal carotid artery of fourteen female Wistar albino rat was cannulated, basal blood samples were drawn and esophagus of each was cannulated with intracath. Hydrochloric acid was subsequently poured through the cannula towards stomach. After 30 minutes, blood gas status was checked in order to see if acidosis occurred or not. The rats which developed acidosis were randomly divided into “tris-hydroxymethyl aminomethane” and “normal saline solution” groups. Later the treatment with IV tris-hydroxymethyl aminomethane or normal saline was initiated. At 30th and 60th minutes of the treatment, pH, PaO2, HCO3, PaCO2 and base deficit parameters were checked through arterial blood gas samples to monitor the efficacy of the treatment. At the end of the second hour of the study, the experiment was finalized and the survival of rats was documented.

Results
Following the development of experimental metabolic acidosis in rats by ingestion of intragastric hydrochloric acid, four rats in tris–hydroxymethyl aminomethane and six rats in normal saline group died prior to the end of the follow-up period.
This finding was statistically significant. There was no statistically significant difference between tris–hydroxymethyl aminomethane and normal saline groups with regard to body temperature, blood pressure, heart rate, PaCO2 and PaO2. However, the comparison of two groups with respect to survival indicated a significant difference (p<0.05). pH at 60th minute in tris–hydroxymethyl aminomethane group was significantly lower compared to that in normal saline group (p<0.05), and base deficit values at 30th and 60th minutes in tris–hydroxymethyl aminomethane group were significantly higher compared to that in normal saline group (p<0.05).
Conclusion:
In this experimental rat model, we observed that treatment with IV tris–hydroxymethyl aminomethane prolonged the survival of rats with experimental metabolic acidosis created by ingestion of hydrochloric acid compared to the treatment with normal saline solution.

Keywords

Metabolic acidosis,, tris–hydroxymethyl aminomethane, sodium bicarbonate

Supporting Institution

yok

Project Number

yok

Thanks

We would like to thank to Çiğdem Akalın Akkök, MD, PhD from the Department of Immunology and Transfusion Medicine, Section of Immunohematology, Oslo University Hospital, Ullevaal, Oslo, Norway for helping us to obtain the vials of tris-hydroxymethyl aminomethane (Tribonat™) from Norway, since it is unavailable in the medical market of Turkey.

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