TLC and GC-MS analysis of petroleum ether fraction of fermented wood "Nikhra" of Acacia seyal

Year 2019, Volume: 7 Issue: 1, 23 - 29, 28.02.2019

Abdalbasit Marıod

https://doi.org/10.31195/ejejfs.472038

Cited By: 2

Abstract

Aim of this study to detect organoleptically Acacia seyal fractions (petroleum ether, chloroform, methanol  and aqueous) fermented wood"Nikhra" of the fractions accumulating the strongest sweet fragrance these fragrances were mainly accumulated in the petroleum ether
fermented wood "Nikhra" fraction and analysis it by using
chromatographic and spectroscopic analysis. Petroleum ether fermented wood "Nikhra" fraction analysis with TLC and spray TLC with vanillin H₂SO₄ (pink) (C1, C2,C3,C4,C5,C6,C7), Rf values
(0.92, 0.86, 0.71, 0.64, 0.57, 0.50, 0.36) ,were expected to be phenolic, with vanillin HCL (red) compounds spots (C5,C6,C7) with Rf values (0.57, 0.50, 0.37) was expected to be catechin
and with vanillin H₃PO₄, blue-violet zones compounds
spots (C6, C7), with
Rf values (0.50, 0.36), were expected to be lignans. Petroleum ether fermented wood"Nikhra" fraction was divided into two types of compounds
classes aromatic and non aromatic by http://research.easybib.com and hence compounds were classified to phenolics and terpenoids compounds by GC/MS. Fragrant
aromatics or terpenoids were targeted in this part of study. GC-MS analysis
gave a spectrum of fragrance aromatic compounds (phenolics) in the petroleum
ether fermented wood "Nikhra" fractions of A.seyal, was Petadecanoic
acid(5.64%) and Tetracosamethyl-cyclododecasiloxane(4.17%) total fragrance
aromatic compounds(44.57%), and main terponoids compounds was Octadecanoic
acid(2.52) % total fragrance aromatic compounds(11.87%).

Keywords

Acacia seyal, Gas Chromatography (GC)

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