Effect of Intravenous Lipid Emulsion Therapy on Serum Pseudocholinesterase in Experimental Model of Diazinon Intoxication

Abstract

Diazinon ile oluşturulan intoksikasyon modelinde İntravenöz lipit emülsiyon tedavisinin (% 20 lipid solüsyonu) serum pseudokolinesteraz düzeyi üzerine etkisinin araştırılması amaçlandı. Organofosfat zehirlenmeleri acil servise başvuran hastalar arasında önemli bir yer tutmaktadır. En sık kullanılan organik fosforlu bileşikler diazinon, malation, paration'dur. İntravenöz lipit emülsiyon (ILE) tedavisi lokal anesteziklerin yol açtığı sistemik toksisite durumlarında yeni bir tedavi yöntemi olarak kullanılmaktadır. 21 adet Wistar-albino grubu sıçan yedişerli üç eşit gruba ayrıldı. Grup I, kontrol; Grup II, diazinon; Grup III, diazinon + İntravenöz lipit emülsiyon tedavisi grubu olarak düzenlendi. Grup I’e sadece 1 ml mısır yağı gavaj yolu ile verildi. Grup II’ye 335 mg/kg diazinon (gavaj yolu ile) verildi. Grup III’ e diazinon’a ilave olarak kuyruk veninden 3 ml/kg olacak şekilde %20 lipit solüsyonu verildi. Deney sonunda hayvanlardan alınan kanlarda serum psödokolinesteraz düzeyleri bakıldı. Psödokolinesteraz düzeyleri analiz edildiğinde diazinon ile diazinon+lipit grubu arasında istatistiksel açıdan önemli bir fark bulunamadı. Bununla birlikte kontrol grubu ile diğer gruplar arasında önemli farklılık tespit edildi (p < 0.05). Çalışmamızda lipit tedavisinin serum pseudokolinesreraz üzerine olumlu etkisi tespit edilemedi. Daha düşük dozlardaki toksikasyon modelinde yeni çalışmalar denenebilir

Keywords

• Diazinon, psödokolinesteraz, lipid

Effect of Intravenous Lipid Emulsion Therapy on Serum Pseudocholinesterase in Experimental Model of Diazinon Intoxication

Abstract

The aim of this study was to investigate the effect of intravenous lipid emulsion (20% lipid solution) on serum pseudocholinesterase in an toxication model of diazinon. Organophosphate poisoning has a different importance for individuals those admitted to the emergency service due to poisoning. The most commonly used organic phosphorus compounds are diazinon, malathion, and parathion. Intravenous lipid emulsion (ILE) treatment is used as a new treatment method in systemic toxicity cases caused by local anesthetics. Twenty-one male Wistar albino rats, (weighing 180&ndash;200 g) were randomly divided into three equal groups. Group I was control, while Group II was diazinon and Group III was diazinon + lipid emulsion treatment. Only 1 ml corn oil was given by gavage to the rats in Group I. A diazinon dose of 335 mg/kg was given by gavage to the rats in Group II. In addition to diazinon, a 20% lipid solution (3 ml/kg) was administered via tail vein to the rats in Group III. At the end of the experimental period, blood samples were taken from animals, and serum pseudocholinesterase levels were measured. When the pseudocholinesterase levels were analyzed, no significant difference was found between diazinon and diazinon + lipid emulsion treatment groups. However, a significant difference was found between control and the others (p < 0.05). In our study, there was no positive effect of lipid treatment detected on serum pseudocholinesterase. Toxication models with lower doses can be designed in future studies.

Keywords

• Diazinon, pseudocholinesterase, lipid

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