Comparison of the Effects of Alpha Lipoic Acid and Vitamin C on Colonic Anastomosis in the Rat Sepsis Model

Abstract

Certain conditions, mainly peritoneal contamination, are considered to be risky for anastomosis. In our study, the effect of vitamin C and lipoic acid combination on anastomosis healing following colonic anastomosis performed in rats with experimentally-induced intraabdominal sepsis has been investigated. A total of 48 male Sprague Dawley albino rats weighing between 200-250 gram were included in the study and allocated to 6 groups with 8 rats in each. The rats in the control group were given only routine enteral feed in the preoperative and postoperative periods. The rats in the vitamin C group were given 100 mg/kg vitamin C IV/S.C, the rats in the lipoic acid group were given 50 mg/kg lipoic acid daily via the peroral route, and the rats in the vitamin C + lipoic acid group were given vitamin C and lipoic acid via the same routes beginning from the operation day. Perforation was induced in the sigmoid colon and intraabdominal sepsis was induced, and this perforation site was resected 24 hours later and an end-to-end colonic anastomosis was performed. The anastomosis site was taken out of the abdomen and the bursting pressure was measured. Biochemical analysis was performed for this site in order to analyze the prolidase enzyme, hydroxyproline and malondialdehyde thereafter. While the mean bursting pressures and hydroxyproline levels of the combination group were found to be statistically significantly higher than those of the other study groups (p

Keywords

• Rat, intraabdominal sepsis, anastomosis, vitamin C, lipoic acid

Comparison of the Effects of Alpha Lipoic Acid and Vitamin C on Colonic Anastomosis in the Rat Sepsis Model

Öz

Periton kontaminasyonun anastomoz için risk olarak kabul edilir. Bizim çalışmamızda, deneysel kaynaklı karın içi sepsis ile sıçanlarda yapılan kolon anastomoz iyileşmesi üzerine C vitamini ve lipoik asit kombinasyonunun etkisi araştırılmıştır.Kontrol grubundaki sıçanlar preoperatif ve postoperatif dönemde sadece rutin enteral besleme verildi.C vitamini grubundaki fareler, 100 mg/kg vit C IV/SC verildi, lipoik asit grubunda farelere oral yoldan her gün 50 mg/kg alfa lipoik asit verildi ve C vitamini+lipoik asit alan grupta farelere operasyon gününden itibaren aynı yol ile C vitamini ve lipoik asit verildi. Perforasyon sigmoid kolonda oluşturularak karın içi sepsis meydanagetirildi. Bu perforasyon sitesi 24 saat sonra rezeke edildi ve uç uca kolon anastomoz yapıldı. Anastomoz yeri karın dışına alındı ve patlama basıncı ölçüldü. Biyokimyasal olarak prolidaz enzimi, hidroksiprolin ve malondialdehit bu site için analiz edildi. Kombinasyon grubunda patlama basıncı ve hidroksiprolin düzeyleri diğer gruplardan istatistiksel olarak anlamlı derecede yüksekti (p <0,001). Prolidaz ve malondialdehit düzeyleri kombinasyon grubunda diğer gruplarına göre istatiksel olarak dfüşük bulundu. Antioksidan olarak vit C ve lipoik asidin kombine kullanımı dah iyi anastomoz iyileşmesi ve gerginlik sağlamaktadır

Anahtar Kelimeler

• Rat, karın içi sepsis, anastomaz, vit C, lipoik asit

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