THE EFFECT OF ENDOSULFAN ON LACTIC DEHYDROGENASE ENZYME SYSTEM IN LIVER OF MUS MUSCULUS : A HISTOCHEMICAL STUDY

Abstract

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THE EFFECT OF ENDOSULFAN ON LACTIC DEHYDROGENASE ENZYME SYSTEM IN LIVER OF MUS MUSCULUS : A HISTOCHEMICAL STUDY

Abstract

Aim: Endosulfan is an important toxic agent which generates free oxygen radicals in liver. In Çukurova region, some relation between the incidence of toxic injury to liver and widespread use of endosulfan has been suggested. Since endosulfan is a frequently used pesticide, it was planned to investigate whether endosulfan could do liver injury and increase lactic dehydrogenase in liver. Methods: Sixty mature (30 controls, 30 experimental) Mus musculus, weighing between 2340 g, obtained from Medical Experimental Surgery Research Center of Çukurova University, were utilized in this study. The effects of oral administration of endosulfan (0.24 mg per 100 g body weight) daily for 90 days (short term) and 180 days (long term) were investigated. Results: At the end of the experimental period, no effect on body weights but an increase on liver weight and hepato/somatic index were observed in both short and long terms. On microscopic examination, livers of experimental groups exhibited severe damage histopathologically. On the other hand, livers of the same group, particularly in long term oral endosulfan administered mice, demonstrated some toxic changes and high levels of lactic dehydrogenase activity with enzyme histochemical method. Conclusion: The exposure to endosulfan in mice caused liver tissue damage histologically, this was also demonstrated by hepatic/somatic index and increased levels of liver LDH assesed with histochemical method

Keywords

• Endosulfan, mus musculus, lactic dehydrogenase, enzyme histochemistry

References

1. Kitchin KT. An enzymatic approach to biotransformation. Meth Find Exptl Clin Pharmacol 1984;6:303-10
2. Kurutas EB, Tuncer I. A mouse model for evaluating the induction of liver glucose-6-phosphate dehydrogenase by halothane. Turk J Vet Anim Sci 2000;24: 511-5
3. Kurutas EB, Doran F, Varinli S. The refererance value of antioxidant status in erythrocyte and liver Saglıklı erkek farelerde eritrosit ve karaciger antioksidan degerleri. Turk J Vet Anim Sci 2001;25: 505-9 referans
4. Liang HCL, Shertzer HG, Nebert DW. “Oxidative stress” response in liver of a treated newborn mouse having a 1.2- centimorgan deletion on chromosome 7. Biochem Biophys Res Commun 1992; 182:1160-64
5. Tietz W. Fundamentals of Clinical Chemistry (Eds. CA. Burtis and ER. Ashwood) W.B. Saunders Comp. Philadelphia, London, Toronto, Montreal, Sydney, Tokyo 1999,pp:803-4
6. Maqvi SM, Vaishnai C. Bioaccumulative potential and toxicity of endosulfan insecticide to non-target animals. Comp Biochem Physiol 1993;105:347-61
7. Bancroft JD, Stevens A. Theory and Practice of Histological Techniques. Churchill Livingstone Comp. Edinburg 1977,pp:1-15
8. Gill TS, Pande J, Tewart H. Effects of endosulfan on the blood and organ chemistry of freshwater fish. Ecotoxicol Enviromental Saf 1991; 21: 80-91

9. Gill TS, Pande J, Teward H. Effect of endosulfan on the blood and organ chemistry of freshwater fish. Ecotoxicol Enverimental Saf 1991;21: 80-91
10. Moulin F, Copple BL, Ganey PE, Roth RA. Hepatic and extrahepatic factors critical for liver injury during lipopolysacharide exposure. Am J Physiol Gastrointest Liver Physiol 2001;281:6:1423-31
11. Hasewaga R, Cabral R, Hoshiya T, Hakoi K, Ogiso T, Boony Aphipta P, Sharia T, Ito N. Carcinogenic potential of some pesticides in a medium-term multi organ bioassay in rats. Int J Cancer 1993;54: 489-93
12. Wade MG, Foster WG, Younglai EV, et al. Effects of subchronic exposure to a complex mixture of persistent contaminants in male rats: systemic, immune, and reproductive effects. Toxicol Sci 2002;67:131-43
13. Choudhary N, Sharma M, Verma P, Joshi SC. Hepato and nephrotoxicity in rat exposed to endosulfan. J Environ Biol 2003;24:305-8
14. Barlas NE. The effect of commercial and microorganism-degraded solutions of endosulfan and carbamyl on albino mice. Tr J of Zoology, 1994;18:221-6
15. Misra VK, Srivastava MK, Raizada RB. Testicular toxicity of fungicide and rat: morphological and biochemical evaluations. Ind Health 1993;31:59-67
16. Szbo L, Matkovich B, Barabas K, Oroszlan GY. Effects of various thiols on paraquat toxicity. Comp Biochem Physiol 1986;83:149-53