TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus among Egyptian patients

Afaf Elsaid; Mohamed Helaly; El-sayed Hatata; Osama Fouda; Ahmad Settin

TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus among Egyptian patients

Year 2012, Volume: 9 Issue: 3, 173 - 177, 01.09.2012

Afaf Elsaid Mohamed Helaly El-sayed Hatata Osama Fouda Ahmad Settin

Abstract

To explore the association of TNF-α-308 and IFN- γ +874 genetic polymorphisms with type 2 diabetes mellitus (DM) in Egyptian cases. Participants included 207 cases with type 2 DM, 93 males and 114 females. Their mean age was 57 years. They were compared to a control group of 212 healthy unrelated subjects from the same locality. DNA was amplified using PCR with sequence specific primers for detection of polymorphism related to TNF-α-308 (G/A) and IFN-γ+874 (A/T). The combined homozygote pattern of TNF-α-308 (AA) and IFN-γ+874 (A/A) was significantly higher in diabetic cases versus controls (p=0.023) that was manifest (although non-significant) among complicated, uncontrolled cases and cases with marked insulin resistance. Polymorphism related to TNF-α-308 AA and IFN-γ+874 AA may be considered as a genetic biomarker for type 2 DM in Egyptian subjects with potential impact on family counseling and management.

Keywords

TNF-α-308, IFN- γ +874, type 2 DM, gene polymorphism, Egypt Mısırlı

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