GnRH Agonist Stimulate Oocyte Number And Maturation In Mice Superovulated With eCG AND hCG

Year 2005, Volume: 2 Issue: 1, 1 - 4, 01.03.2005

Ömer Coşkun Mehmet Kanter

Abstract

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Keywords

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GnRH Agonist Stimulate Oocyte Number And Maturation In Mice Superovulated With eCG AND hCG

Abstract

Aim: This study was designed to investigate the effects of gonadotropin-releasing hormone agonist (GnRHa) on the ovulation rate, the oocyte number and maturation in mice superovulated with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG). Methods: Thirty female mice, aging 3 months and weighing between 25-30 g, were assigned to the three experimental groups of control, superovulated and GnRHa treated, each containing 10 animals. Control mice were received only the intraperitoneal (i.p.) injection of 0.1 ml 0.9% NaCl. Superovulated mice were given an i.p. injection of 5 IU of eCG followed 48 hr later by 5 IU of hCG injection (i.p.). The GnRHa treated mice were received an intramuscular (i.m.) injection of a 20 mg/kg GnRHa (buserelin) 24 hr before the superovulation with eCG -hCG. Thirteen hr after hCG administration, mice were sacrificed by cervical dislocation. The ovaries and the fallopian tubes were also harvested for counting the corpora lutea and cumulus-enclosed oocytes. Results: The oocyte number and the maturation, ovulation rate, and the corpus luteum number were higher in GnRHa treated mice than controls and superovulated mice. Conclusion: It is concluded that an i.m. injection of the GnRHa 24 hr before the superovulation with eCG-hCG increases the oocyte number and maturation, and ovulation rate, thus, could be used for in vivo and in vitro fertilizations. Keywords: GnRH agonist, Superovulation, Oocyte maturation, Mice.

Keywords

GnRH agonist, Superovulation, Oocyte maturation, Mice

References

• Hsueh AJ, Jones PB. Extrapituitary action of Endocrine Rev 1981;2:437-61 hormone.
• Dekel N, Lewysohn O, Ayalon D, Hazum E. Receptors for gonadotropin releasing hormone are present in rat oocytes. Endocrinology 1988;123:1205-7
• Yang BC, Uemura T, Minaguchi H. Effects hormone agonist on oocyte maturation, fertilization and embryonal development of mice. Journal of Assisted Reproduction and Genetics 1995;12:728-32 releasing
• Zanagnolo V, Dharmarajan AM, Hesla J, Wallach EE. Effects of a gonadotropin- releasing hormone analog on rabbit ovarian function. Endocrinology 1996; 137:5400-6
• Lindner HR, Tsafriri A, Lieberman ME et al. Gonodotrophin action on cultured Graafian follicles: induction of maturation division of the mammalian oocyte and differentitation of the luteal cell. Recent Prog Horm Res 1974;30:79
• Yoshimura Y, Nakamura Y, Yamada H et al. Gonadotropin-releasing hormone agonists induce meiotic maturation and degeration of oocytes in the in vitro perfused rabbit ovary. Fertility and Sterility 1991;55: 177-83
• Yoshimura Y, Nakamura Y, Oda T et al. Effects of gonadotropin-releasing hormone agonists on meiotic maturation of follicle-enclosed oocytes in rabbits. Biology of Reproduction 1990;43:1012-8
• Clayton RN, Catt KJ. Gonadotropin- releasing characterization, physiological regulation and relationship to reproductive function. Endocrine Rev 1981;2:186-209 receptors:
• Quinn P, Kerin JF, Warnes GM. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertility and Sterility 1985;44:493-8
• Clayton RN, Harwood JP, Catt KJ. Gonadotropin-releasing analogue binds to luteal cells and inhibits progesterone production. Nature 1979;282:90-2 hormone
• Clark MR, Thibier C, Marsh JM, LeMaire WJ. accumulation by luteinizing hormone- releasing hormone (LHRH) and LHRH analogs in rat granulosa cells in vitro. Endocrinology 1980;107(1):17-23
• Yoshimura Y, Nakamura Y, Ando M et al. Direct effect of gonadotropin-releasing hormone agonists on the rabbit ovarion follicle. Fertility and Sterility 1992;57: 1091-7
• Koves K, Gottschall PE, Arimura A. Gonadotropin-releasing hormone binding sites in ovaries of normal cycling and persistent-estrus rats. Biology and Reproduction 1989;41:505-11
• Yoshimura Y, Nakamura Y, Oda T et al. Induction of meiotic maturation of follicle-enclosed oocytes of rabbits by a transient increase followed by an abrupt decrease in cyclic AMP concentration. J Reprod Fertil 1992;95:803-12