Electrophysiological evidence of neuronal action potential alterations by a hydroxycinnamic acid derivative at the peripheral level

Feyza Alyu Altınok; Abderaouf Boubekka; Ahmed Hasan; Ilhem Dallali; Yusuf Burak; Nilgün Öztürk; Yusuf Ozturk

doi:10.55971/EJLS.1712839

Electrophysiological evidence of neuronal action potential alterations by a hydroxycinnamic acid derivative at the peripheral level

Abstract

Hydroxycinnamic acid derivatives have emerged as promising agents in pain management due to their multifaceted actions on various processes, including neuronal excitability. In this study, we investigated the electrophysiological effects of ferulic acid, a hydroxycinnamic acid derivative, on primary cultured dorsal root ganglion neurons using the whole-cell patch-clamp technique. Acute application of ferulic acid at a dose of 100 µM to the external solution resulted in a significant hyperpolarization of the resting membrane potential, indicating a stabilization of the neuronal membrane and a reduction in baseline excitability. Furthermore, ferulic acid induced a more negative afterhyperpolarization phase, suggesting enhanced potassium conductance and prolonged refractory periods. Notably, the threshold for action potential initiation became less negative following treatment, demonstrating that a stronger depolarizing stimulus was required to elicit neuronal firing. These changes collectively reflect a decrease in neuronal excitability that extends beyond anti-inflammatory or antioxidant effects, highlighting direct modulation of ion channel dynamics as a key mechanism. The findings provide mechanistic insight into the analgesic potential of ferulic acid by demonstrating its ability to alter action potential parameters and suppress excitability in peripheral sensory neurons. This study supports the therapeutic relevance of hydroxycinnamic acid derivatives in the development of novel analgesics targeting peripheral nociceptive pathways.

Keywords

Supporting Institution

This study was supported by the Anadolu University Scientific Research Foundation (AUBAP-2203S021, Eskisehir, Türkiye).

Project Number

AUBAP-2203S021

Ethical Statement

This study was conducted using Sprague-Dawley rats. All procedures involving animals were carried out in full compliance with the European Directive 2010/63/EU on the protection of animals used for scientific purposes. The experimental protocol received approval from the Local Animal Ethics Committee at Anadolu University in Eskisehir, Turkey (Approval No: 2022-11).

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Details

Primary Language

English

Subjects

Basic Pharmacology

Journal Section

Research Article

Authors

Feyza Alyu Altınok
0000-0001-9146-3841
Türkiye

Abderaouf Boubekka
0000-0003-3785-0124
Türkiye

Ahmed Hasan
0000-0003-3771-6910
Türkiye

Ilhem Dallali
0000-0001-9876-9106
Belgium

Yusuf Burak
0009-0006-4522-8860
Türkiye

Nilgün Öztürk
0000-0002-7503-0146
Türkiye

Yusuf Ozturk ^*
0000-0002-9488-0891
Türkiye

Publication Date

August 31, 2025

Submission Date

June 3, 2025

Acceptance Date

August 18, 2025

Published in Issue

Year 2025 Volume: 4 Number: 2

DOI

https://doi.org/10.55971/EJLS.1712839

IZ

https://izlik.org/JA28CT35BF

Cite

RIS / Bibtex

APA

Alyu Altınok, F., Boubekka, A., Hasan, A., Dallali, I., Burak, Y., Öztürk, N., & Ozturk, Y. (2025). Electrophysiological evidence of neuronal action potential alterations by a hydroxycinnamic acid derivative at the peripheral level. European Journal of Life Sciences, 4(2), 77-83. https://doi.org/10.55971/EJLS.1712839

AMA

1.Alyu Altınok F, Boubekka A, Hasan A, et al. Electrophysiological evidence of neuronal action potential alterations by a hydroxycinnamic acid derivative at the peripheral level. Eur J Life Sci. 2025;4(2):77-83. doi:10.55971/EJLS.1712839

Chicago

Alyu Altınok, Feyza, Abderaouf Boubekka, Ahmed Hasan, et al. 2025. “Electrophysiological Evidence of Neuronal Action Potential Alterations by a Hydroxycinnamic Acid Derivative at the Peripheral Level”. European Journal of Life Sciences 4 (2): 77-83. https://doi.org/10.55971/EJLS.1712839.

EndNote

Alyu Altınok F, Boubekka A, Hasan A, Dallali I, Burak Y, Öztürk N, Ozturk Y (August 1, 2025) Electrophysiological evidence of neuronal action potential alterations by a hydroxycinnamic acid derivative at the peripheral level. European Journal of Life Sciences 4 2 77–83.

IEEE

[1]F. Alyu Altınok et al., “Electrophysiological evidence of neuronal action potential alterations by a hydroxycinnamic acid derivative at the peripheral level”, Eur J Life Sci, vol. 4, no. 2, pp. 77–83, Aug. 2025, doi: 10.55971/EJLS.1712839.

ISNAD

Alyu Altınok, Feyza - Boubekka, Abderaouf - Hasan, Ahmed - Dallali, Ilhem - Burak, Yusuf - Öztürk, Nilgün - Ozturk, Yusuf. “Electrophysiological Evidence of Neuronal Action Potential Alterations by a Hydroxycinnamic Acid Derivative at the Peripheral Level”. European Journal of Life Sciences 4/2 (August 1, 2025): 77-83. https://doi.org/10.55971/EJLS.1712839.

JAMA

1.Alyu Altınok F, Boubekka A, Hasan A, Dallali I, Burak Y, Öztürk N, Ozturk Y. Electrophysiological evidence of neuronal action potential alterations by a hydroxycinnamic acid derivative at the peripheral level. Eur J Life Sci. 2025;4:77–83.

MLA

Alyu Altınok, Feyza, et al. “Electrophysiological Evidence of Neuronal Action Potential Alterations by a Hydroxycinnamic Acid Derivative at the Peripheral Level”. European Journal of Life Sciences, vol. 4, no. 2, Aug. 2025, pp. 77-83, doi:10.55971/EJLS.1712839.

Vancouver

1.Feyza Alyu Altınok, Abderaouf Boubekka, Ahmed Hasan, Ilhem Dallali, Yusuf Burak, Nilgün Öztürk, Yusuf Ozturk. Electrophysiological evidence of neuronal action potential alterations by a hydroxycinnamic acid derivative at the peripheral level. Eur J Life Sci. 2025 Aug. 1;4(2):77-83. doi:10.55971/EJLS.1712839