Determination of potential probiotic properties of lactic acid bacteria isolated from colostrum milk

Year 2023, Volume: 2 Issue: 1, 31 - 38, 24.04.2023

Pervin Soyer , Melek Tekgöz , Yağmur Tunalı

https://doi.org/10.55971/EJLS.1263343

Abstract

Colostrum milk has been used as a source for isolating many probiotic bacteria since it contains many helpful probiotic microorganisms. Because of the huge microorganism diversity and functionality of colostrum, there is opportunity to isolate bacteria and analyze their probiotic potential. Comparing the diversity of probiotic bacteria in milk from postpartum periods is also crucial. In the current study, Lactic Acid Bacteria (LAB) cultures were isolated from cow colostrum milk. The 28 cultures were isolated, but only 2 were characterized as LAB by their colony and molecular characterization, Gram nature, catalase, antibiotics, and pepsin tolerance. As a result of molecular identification tests, the isolates were identified as Lactobacillus casei and Lactobacillus paracasei with 99-100% homology. These two isolates could survive in the presence of gastric and intestinal conditions. These isolates also showed antimicrobial and antioxidant activities. The results demonstrated that LAB species isolated from colostrum milk exhibited promising probiotic properties and seemed favorable for use in pharmaceuticals and foods.

Keywords

Colostrum, gastrointestinal tolerance, lactobacillus, probiotic, postpartum milk

References

• WHO, World Health Organization. First food. 2019. https://www.who.int/nutrition/topics/world_breastfeeding_week/en/
• Batista da Silva Galdino, A., et al., Bovine colostrum: benefits for the human respiratory system and potential contributions for clinical management of COVID-19. Food and Agricultural Immunology, 2021. 32(1): p. 143-162. https://doi.org/10.1080/09540105.2021.1892594
• FAO/WHO. Guidelines for the evaluation of probiotics in food. London, Ontario, Food and Agriculture Organization of the United Nations and World Health Organization Working Group Report. 2002.
• Shokryazdan P, Sieo CC, Kalavathy R, Liang JB, Alitheen NB, Faseleh Jahromi M, et al. Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains. BioMed research international. 2014;2014. https://doi.org/10.1155/2014/927268
• Prete, R., et al., Beneficial bile acid metabolism from Lactobacillus plantarum of food origin.Scientific reports, 2020. 10(1): p. 1165. https://doi.org/10.1038/s41598-020-58069-5
• Kathade, S., et al., Isolation, characterization, and diversity of probiotic microorganisms from different postpartum milk of various animals. Int. J. Health Sci, 2022. 12(3): p. 223-234. https://doi.org/10.52403/ijhsr.20220332
• Hyrslova, I., et al., Fermentation ability of bovine colostrum by different probiotic strains. Fermentation, 2020. 6(3): p. 93. https://doi.org/10.3390/fermentation6030093
• Khotimah, K., et al., Isolation and Identification Probiotic Candidates Bacteria from Crossbreed Fries Holland (CFH) Colostrum That Grown on Nutrien Agar with Olive and Casein Enrichment. Advance Journal of Food Science and Technology, 2015. 8(1): p. 22-26. https://doi.org/10.19026/ajfst.8.1458
• Lindner, Juliano De Dea, et al. Recovery and identification of bovine colostrum microflora using traditional and molecular approaches. Food Technology and Biotechnology, 2011, 49.3: 364.
• Chen, Zhina, et al. Isolation of exopolysaccharide‐producing bacteria and yeasts from Tibetan kefir and characterisation of the exopolysaccharides. International Journal of Dairy Technology, 2016, 69.3: 410-417. https://doi.org/10.1111/1471-0307.12276
• Ayyash, M., et al., In-vitro investigation into probiotic characterization of Streptococcus and Enterococcus isolated from camel milk. LWT, 2018. 87: p. 478-487. https://doi.org/10.1016/j.lwt.2017.09.019
• Maragkoudakis, P.A., et al., Probiotic potential of Lactobacillus strains isolated from dairy products. International Dairy Journal, 2006. 16(3): p. 189-199. https://doi.org/10.1016/j.idairyj.2005.02.009
• Wilkins, T., et al., Standardized single-disc method for antibiotic susceptibility testing of anaerobic bacteria. Antimicrobial Agents and Chemotherapy, 1972. 1(6): p. 451-459. https://doi.org/10.1128/aac.1.6.451
• Clinical and Laboratory Standards Institute (2012). Reference Method for Antimicrobial Disk Susceptibility Tests; Approved Standard—Eleventh Edition (2012). CLSI document 29(1).
• Liu, Wenjun, et al. Characterization of potentially probiotic lactic acid bacteria and bifidobacteria isolated from human colostrum. Journal of Dairy Science, 2020, 103.5: 4013-4025. https://doi.org/10.3168/jds.2019-17602
• Blois, Marsden S. Antioxidant determinations by the use of a stable free radical. Nature, 1958, 181: 1199-1200.
• Haghshenas, B., et al., Bioactivity characterization of Lactobacillus strains isolated from dairy products. Microbiologyopen, 2015. 4(5): p. 803-813. https://doi.org/10.1002/mbo3.280
• Martin, V., et al., Inhibition of human immunodeficiency virus type 1 by lactic acid bacteria from human breastmilk. Breastfeeding Medicine, 2010. 5(4): p. 153-158. https://doi.org/10.1089/bfm.2010.0001
• Soleymanzadeh, N., Mirdamadi, S., Kianirad, M. Antioxidant activity of camel and bovine milk fermented by lactic acid bacteria isolated from traditional fermented camel milk (Chal). Dairy Science & Technology, 2016, 96: 443-457. https://doi.org/10.1007/s13594-016-0278-1
• Angmo, K., A. Kumari, and T.C. Bhalla, Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. LWT-food Science and Technology, 2016. 66: p. 428-435. https://doi.org/10.1016/j.lwt.2015.10.057
• Nawaz, Muhammad, et al. Screening and characterization of new potentially probiotic lactobacilli from breast-fed healthy babies in Pakistan. African Journal of Microbiology Research, 2011, 5.12: 1428-1436. https://doi.org/10.5897/AJMR10.737
• Lee, YK., et al. Permanent colonization by Lactobacillus casei is hindered by the low rate of cell division in mouse gut. Applied and Environmental Microbiology, 2004, 70.2: 670-674. https://doi.org/10.1128/AEM.70.2.670-674.2004
• Somashekaraiah, R., et al., Probiotic properties of lactic acid bacteria isolated from neera: a naturally fermenting coconut palm nectar. Frontiers in Microbiology, 2019. 10: p. 1382. https://doi.org/10.3389/fmicb.2019.01382
• Forestier, Christiane, et al. Probiotic activities of Lactobacillus casei rhamnosus: in vitro adherence to intestinal cells and antimicrobial properties. Research in microbiology, 2001, 152.2: 167-173.  https://doi.org/10.1016/s0923-2508(01)01188-3.
• Vitola, H.R.S., et al., Probiotic potential of Lactobacillus casei CSL3 isolated from bovine colostrum silage and its viability capacity immobilized in soybean. Process Biochemistry, 2018. 75: p. 22-30. https://doi.org/10.1016/j.procbio.2018.09.011
• Giraffa, G., N. Chanishvili, and Y. Widyastuti, Importance of lactobacilli in food and feed biotechnology. Research in microbiology, 2010. 161(6): p. 480-487. https://doi.org/10.1016/j.resmic.2010.03.001
• Kapila, S. and V.P. Sinha, Antioxidative and hypocholesterolemic effect of Lactobacillus casei ssp casei (biodefensive properties of lactobacilli). Indian journal of medical sciences, 2006. 60(9): p. 361-370.
• Mishra, V., et al., Probiotics as potential antioxidants: a systematic review. Journal of agricultural and food chemistry, 2015. 63(14): p. 3615-3626. https://doi.org/10.1021/jf506326t
• Siragusa, S., et al. Synthesis of γ-aminobutyric acid by lactic acid bacteria isolated from a variety of Italian cheeses. Applied and environmental microbiology, 2007, 73.22: 7283-7290. https://doi.org/10.1128/AEM.01064-07