Characterization and Biofilm Inhibition Properties of Astragalus gummifer Crude

Year 2025, Volume: 4 Issue: 1, 37 - 44, 13.07.2025

Ruhane Tosunoğlu , Muhammed Kürşad Coşkun , Aleyna Ertürk , Ayşenur Yazıcı

Abstract

Astragalus species are widely recognized for their potent bioactive properties; however, the antibiofilm potential
of Astragalus gummifer remains unexplored. In the present study, we assessed the antimicrobial and antibiofilm
activity of A. gumnifer metabolic extracts against referenced bacterial and Candida strains. Plant material was
collected from the Palandöken Mountain region in Erzurum, Turkey. The crude extract was obtained by methanol
extraction and concentrated at 62 °C, followed by dissolution in distilled water for microdilution and crystal violet
assays. In addition to these assays, untargeted metabolite profiling was performed on the methanolic extract.
Fourier Transform Infrared Spectroscopy (FT-IR) and Quadrupole Time-of-Flight Liquid Chromatography–Mass
Spectrometry (Q-TOF-LC/MS) analyses were employed for compound identification. A total of nine known
secondary metabolites were successfully identified in the crude extract of A. gummifer.

Keywords

Astragallus gumnifer, , biofilm , Qtof lcms , Kristal violet

Project Number

2209-A

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