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Melatonin and cisplatin synergistically enhance apoptosis via autophagy-dependent alteration of P53 transcription in human colorectal cancer cells

Year 2022, Volume: 1 Issue: 2, 11 - 18, 01.11.2022

Abstract

Cisplatin is one of the most general chemotherapeutic agents used to treat various cancers, including colorectal and breast
cancer. because cisplatin has some adverse effects including cardiotoxicity and hepatoxicity, it is usage limited. Melatonin is a
natural product that responsible for regulator of circadian rhythms and has anti-cancer potential. However, its synergistic
effects with melatonin and cisplatin, its efficacy in cancer cell death, its mechanisms and biological targets are not well
understood. In this study, it was aimed to determine the synergistic activity of cisplatin with melatonin in colon cancer cell
death through apoptotic and autophagic mechanisms. In the present study, we found that melatonin with cisplatin treatment
did not affect the cytotoxicity, but cisplatin increased in 24 h incubation period. Melatonin and combined treatment of
melatonin and cisplatin also increased the cytotoxicity in 48h incubation period. It was observed that cisplatin treatments used
together with melatonin and melatonin inhibited the mitogen activity of colon cancer cells. In addition, combined treatment of
cisplatin and melatonin and single treatment of cisplatin increased both apoptosis and autophagic cell death. The results
revealed that the use of melatonin with combined cisplatin has been shown to increase the apoptosis and autophagic cell deaths
via P53 gene activation.

References

  • 1- Ribic CM, Sargent DJ, Moore MJ, Thibodeau SN, French AJ, Goldberg RM, et al. Tumormicrosatellite-instability status as a predictor of benefit fromfluorouracil-based adjuvant chemotherapy for colon cancer. N Engl J Med 2003;349;247-257.
  • 2- Jung JH, Shin EA, Kim JH, Sim DY, Lee H, Park JE, et al. NEDD9 Inhibition by miR-25-5p Activation Is Critically Involved in Co-Treatment of Melatoninand Pterostilbene-Induced Apoptosis in Colorectal Cancer Cells. Cancers 2019;11;11;1684
  • 3- Jung JH, Sohn EJ, Shin EA, Lee D, Kim B, Jung DB, et al. MLT Suppresses the Expression of 45S Preribosomal RNA and Upstream Binding Factor and Enhances theAntitumor Activity of Puromycin in MDA-MB-231 Breast Cancer Cells. Evid. Based Complement. Alternat Med 2013;2013;879746.
  • 4- Zdzienicka MZ. Mammalian mutants defective in the response to ionizing radiation-induced DNA damage. Mutat Res 1995;336;203-213.
  • 5- Kubbutat MH, Jones SN, Vousden KH. Regulation of p53 stability by Mdm2. Nature 1997;387;299-303.
  • 6- Uhrinova S, Uhrin D, Powers H, Watt K, Zheleva D, Fischer P, et al. Structure of free MDM2 N-terminal domain reveals conformational adjustments that accompany p53-binding. J Mol Biol 2005;350; 587-598.
  • 7- Ünlü S, Sağlar E. İn vitro Gama Radyasyon Maruziyeti Sonrası Periferal Kan Lenfositlerinde MDM2 Gen Ekspresyon Değişikliğinin Araştırılması. FÜ Sağ Bil Tıp Derg 2012;26;2;87-90.
  • 8- Zieske JD. Expression of Cyclin-Dependent Kinase Inhibitors During Corneal Wound Repair. Progression in Retinal and Eye Research 2000;19;257-270.
  • 9- Bastians H, Townsley FM, Ruderman JV. The Cyclin-Dependent Kinase Inhibitor p27 Kip1 Induces N-terminal Proteolytic Cleavage of Cyclin A. PNAS 1998;95;5374-5381.
  • 10- Ay ME, Terzioğlu O , Terzi C, İzci Ay Ö. Kolorektal kanserlerde, p21, p27, p57 siklin bağımlı kinaz inhibitör geni (CDKI) ekspresyonlarının değerlendirilmesi. Akademik Gatroentereloji Dergisi 2006;5;1;20-25.
  • 11- Liu X, Chen Z, Chua CC, Ma YS, Youngberg GA, Hamdy R, et al. Melatonin as an effective protector against doxorubicin-induced cardiotoxicity. Am. J. Physiol. Heart Circ. Physiol. 2002;283;254-263.
  • 12- Plaimee P, Weerapreeyakul P, Barusrux S, Johns NP. Melatonin potentiates cisplatin‐induced apoptosis and cell cycle arrest in human lung adenocarcinoma cells. Cell Prolif 2015;48;1;67-77.
  • 13- Koner K, Goi T, Hirono Y, Katayama K, Yamaguchi A. Beclin 1 Gene Inhibits Tumor Growth in Colon Cancer Cell Lines. Anticancer Research 2007;27;1453-1457.
  • 14- Talib WH, Alsayed AR, Abuawad A, Daoud S, Mahmod AI. Melatonin in Cancer Treatment. Current Knowledge and Future Opportunities 2021;26;9;2506.
  • 15- Scott AE, Cosma GN, Frank AA, Wells RL, Gardner HS. Disruption of mitochondrial respiration by melatonin in MCF-7 cells. Toxicol Appl Pharmacol 2001;171;149-156.
  • 16- Hosseini F, Shanehbandi D, Soleimanpour J, Yousefi B, Alemi F. Melatonin Increases the Sensitivity of Osteosarcoma Cells to Chemotherapy Drug Cisplatin. Drug Research 2022;72;6;312-318.
  • 17- Menon M, Dhamija S. Beclin 1 Phosphorylation at the Center of Autophagy Regulation. Front Cell Dev Biol 2018;6;137.
  • 18- Xia F, Liu P, Li M. The regulatory factors and pathological roles of autophagy-related protein 4 in diverse diseases recent research advances. Med Res Rev 2021;41;3;1644-1675.
  • 19- Han Z, Wei W, Dunaway S, Darnowski JW, Calabresi P, Sedivy J, Hendrickson EA, Balan KV, Pantazis P, Wyche JH. Role of p21 in Apoptosis and Senescence of Human Colon Cancer Cells Treated with Camptothecin. J of Biol Chem 2002;277;19;17154-60.
  • 20- Levine AJ. Spontaneous and inherited TP53 genetic alterations. Oncogene 2021;40; 5975-5983.
Year 2022, Volume: 1 Issue: 2, 11 - 18, 01.11.2022

Abstract

References

  • 1- Ribic CM, Sargent DJ, Moore MJ, Thibodeau SN, French AJ, Goldberg RM, et al. Tumormicrosatellite-instability status as a predictor of benefit fromfluorouracil-based adjuvant chemotherapy for colon cancer. N Engl J Med 2003;349;247-257.
  • 2- Jung JH, Shin EA, Kim JH, Sim DY, Lee H, Park JE, et al. NEDD9 Inhibition by miR-25-5p Activation Is Critically Involved in Co-Treatment of Melatoninand Pterostilbene-Induced Apoptosis in Colorectal Cancer Cells. Cancers 2019;11;11;1684
  • 3- Jung JH, Sohn EJ, Shin EA, Lee D, Kim B, Jung DB, et al. MLT Suppresses the Expression of 45S Preribosomal RNA and Upstream Binding Factor and Enhances theAntitumor Activity of Puromycin in MDA-MB-231 Breast Cancer Cells. Evid. Based Complement. Alternat Med 2013;2013;879746.
  • 4- Zdzienicka MZ. Mammalian mutants defective in the response to ionizing radiation-induced DNA damage. Mutat Res 1995;336;203-213.
  • 5- Kubbutat MH, Jones SN, Vousden KH. Regulation of p53 stability by Mdm2. Nature 1997;387;299-303.
  • 6- Uhrinova S, Uhrin D, Powers H, Watt K, Zheleva D, Fischer P, et al. Structure of free MDM2 N-terminal domain reveals conformational adjustments that accompany p53-binding. J Mol Biol 2005;350; 587-598.
  • 7- Ünlü S, Sağlar E. İn vitro Gama Radyasyon Maruziyeti Sonrası Periferal Kan Lenfositlerinde MDM2 Gen Ekspresyon Değişikliğinin Araştırılması. FÜ Sağ Bil Tıp Derg 2012;26;2;87-90.
  • 8- Zieske JD. Expression of Cyclin-Dependent Kinase Inhibitors During Corneal Wound Repair. Progression in Retinal and Eye Research 2000;19;257-270.
  • 9- Bastians H, Townsley FM, Ruderman JV. The Cyclin-Dependent Kinase Inhibitor p27 Kip1 Induces N-terminal Proteolytic Cleavage of Cyclin A. PNAS 1998;95;5374-5381.
  • 10- Ay ME, Terzioğlu O , Terzi C, İzci Ay Ö. Kolorektal kanserlerde, p21, p27, p57 siklin bağımlı kinaz inhibitör geni (CDKI) ekspresyonlarının değerlendirilmesi. Akademik Gatroentereloji Dergisi 2006;5;1;20-25.
  • 11- Liu X, Chen Z, Chua CC, Ma YS, Youngberg GA, Hamdy R, et al. Melatonin as an effective protector against doxorubicin-induced cardiotoxicity. Am. J. Physiol. Heart Circ. Physiol. 2002;283;254-263.
  • 12- Plaimee P, Weerapreeyakul P, Barusrux S, Johns NP. Melatonin potentiates cisplatin‐induced apoptosis and cell cycle arrest in human lung adenocarcinoma cells. Cell Prolif 2015;48;1;67-77.
  • 13- Koner K, Goi T, Hirono Y, Katayama K, Yamaguchi A. Beclin 1 Gene Inhibits Tumor Growth in Colon Cancer Cell Lines. Anticancer Research 2007;27;1453-1457.
  • 14- Talib WH, Alsayed AR, Abuawad A, Daoud S, Mahmod AI. Melatonin in Cancer Treatment. Current Knowledge and Future Opportunities 2021;26;9;2506.
  • 15- Scott AE, Cosma GN, Frank AA, Wells RL, Gardner HS. Disruption of mitochondrial respiration by melatonin in MCF-7 cells. Toxicol Appl Pharmacol 2001;171;149-156.
  • 16- Hosseini F, Shanehbandi D, Soleimanpour J, Yousefi B, Alemi F. Melatonin Increases the Sensitivity of Osteosarcoma Cells to Chemotherapy Drug Cisplatin. Drug Research 2022;72;6;312-318.
  • 17- Menon M, Dhamija S. Beclin 1 Phosphorylation at the Center of Autophagy Regulation. Front Cell Dev Biol 2018;6;137.
  • 18- Xia F, Liu P, Li M. The regulatory factors and pathological roles of autophagy-related protein 4 in diverse diseases recent research advances. Med Res Rev 2021;41;3;1644-1675.
  • 19- Han Z, Wei W, Dunaway S, Darnowski JW, Calabresi P, Sedivy J, Hendrickson EA, Balan KV, Pantazis P, Wyche JH. Role of p21 in Apoptosis and Senescence of Human Colon Cancer Cells Treated with Camptothecin. J of Biol Chem 2002;277;19;17154-60.
  • 20- Levine AJ. Spontaneous and inherited TP53 genetic alterations. Oncogene 2021;40; 5975-5983.
There are 20 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other)
Journal Section Research Articles
Authors

Süleyman Polat This is me

Halime Topal This is me 0000-0002-9152-9027

Nevra Aydemir Celep This is me 0000-0003-1608-5881

Elif Erbaş This is me 0000-0003-1750-3889

Adem Kara This is me 0000-0002-5766-6116

Publication Date November 1, 2022
Published in Issue Year 2022 Volume: 1 Issue: 2

Cite

APA Polat, S., Topal, H., Aydemir Celep, N., Erbaş, E., et al. (2022). Melatonin and cisplatin synergistically enhance apoptosis via autophagy-dependent alteration of P53 transcription in human colorectal cancer cells. Eurasian Journal of Molecular and Biochemical Sciences, 1(2), 11-18.