Investigation of the Effects of Metformin and Ifosfamide Applications on Cell Proliferation and Apoptosis in Hepatocellular Carcinoma Cells

Year 2024, Volume: 3 Issue: 1, 17 - 24, 23.07.2024

Betül Nur Güney , Halime Topal Kızıloğlu , Kübra Solak , Derya Ulusoy , Adem Kara

Abstract

Cancer is a concern, for health ranking as the second leading cause of death after cardiovascular diseases. Among types of cancers hepatocellular carcinoma (HCC) is particularly noteworthy being the most common type following lung and stomach cancers. Various treatment approaches are used for managing HCC like chemotherapy. In this study the HepG2 cancer cell line was used as a model to investigate the effects of ifosfamide and metformin on cancer cells. Cell viability tests and RT PCR analysis targeting genes associated with the PI3K/AKT/mTOR pathway were conducted to evaluate these effects. These results offer valuable insights for advancing further research for their potential application in the treatment not only of HCC but also of other cancer types.

Keywords

Hepatocellular Cancer, Metformin, Ifosfamide, Cell Proliferation, PI3K/AKT/mTOR pathway

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