Investigation of TNF-α and NF-κB Levels in Masseter Muscle of Rats with High Fructose Corn Syrup-induced Metabolic Syndrome

Year 2023, Volume: 2 Issue: 4, 77 - 82, 25.12.2023

Ömer Ekici , Mehmet Bilgehan Pektaş

Abstract

Objective
In recent years, especially due to excessive sugar consumption, metabolic Syndrome (MetS) has become increasingly common
in the world and in Turkey. The aim of the study was to investigate the inflammatory effects of high fructose corn syrup (HFCS)
intake on the masseter muscle of young rats.
Materials and methods
Sixteen 3-week-old male Wistar rats were randomly split into two groups: control and HFCS. Animals were given HFCS in the
form of 20% solutions in drinking water. Animals were sacrificed in the eighth week. Right masseter muscle was isolated for
immunohistochemical and histopathological examination, and left masseter muscle was isolated for gene expression analysis.
Results
Both ELISA and real-time polymerase chain reaction (rt-PCR) measurements revealed that the HFCS group had significantly
higher TNF-a and NF-kB levels than the control group (p <0.05). Additionally, when comparing the HFCS group to the control
group, a higher degree of lymphocyte infiltration was seen.
Conclusion
According to study results, young rats’ masseter muscle tissue had significantly higher levels of TNF- and NF-B due to fructoseinduced
MetS. These findings suggest that MetS, through increased inflammation, can cause masseter muscle dysfunction,
injury, fatigue, and pain.

Keywords

Metabolic syndrome, masseter muscle, inflammation, TNF-a, NF-κB.

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