The Effect of Vitamin C on Histamine and Dust Mite Epicutaneous Test Responses: A Randomized Control Trial

Wan Abdul Aziz Wan Azhar; Husna Hasri; Sze Ying Tan; Divaharan Subramanie; Anasuhah Musa; Farah Dayana Zahedi; Samirah Taufiq Abdul Wahid; Norwahidah Abdul Karim; Shamsul Azhar Shah; Aneeza W Hamizan; Salina Husain

The Effect of Vitamin C on Histamine and Dust Mite Epicutaneous Test Responses: A Randomized Control Trial

Abstract

Objective: Vitamin C has antihistamine properties, but its e!ect on allergic skin responses is unclear. This study aimed to evaluate the e!ect of vitamin C on skin wheal reactions to exogenous histamine and a known allergen during skin prick tests (SPTs). Methods: In this single-blinded randomized controlled trial, adults with allergic rhinitis and known positive SPT toward house dust mites were randomized into 2 groups: the intervention group received vitamin C (1000 mg daily for 7 days) and the control group kept a diet journal only. Repeat testing was conducted on day 8, and wheal sizes were measured using Image J software. Mean di!erences in wheal area (mm") between groups were compared, and treatment e!ect sizes within groups were calculated using Cohen’s d formula. Results: Of 103 participants, 44 in the vitamin C group and 45 in the control group completed the study. Vitamin C led to a small, non-signi#cant reduction in histamine-induced wheal size (!2.5 ± 11.57 mm", Cohen’s d = 0.30 vs. !0.92 ± 14.24 mm", P = .56, Cohen’s d = 0.08), but no reduction was seen for dust mite allergens (+4.48 ± 25.08 mm", Cohen’s d = !0.20 vs. !0.16 ± 26.17 mm", Cohen’s d = 0.01, P = .40). Conclusion: Short-term vitamin C supplementation had a minor, non-signi#cant suppressing e!ect on exogenous histamine but did not attenuate the skin allergen responses. Vitamin C may be useful for histamine intolerance, but this requires larger studies.

Keywords

References

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Details

Primary Language

English

Subjects

Allergy

Journal Section

Research Article

Authors

Wan Abdul Aziz Wan Azhar This is me
Malaysia

Husna Hasri This is me
Malaysia

Sze Ying Tan This is me
Malaysia

Divaharan Subramanie This is me
Malaysia

Anasuhah Musa This is me
Malaysia

Farah Dayana Zahedi This is me
Malaysia

Samirah Taufiq Abdul Wahid This is me
Malaysia

Norwahidah Abdul Karim This is me
Malaysia

Shamsul Azhar Shah This is me
Malaysia

Aneeza W Hamizan This is me
Malaysia

Salina Husain This is me
Malaysia

Publication Date

April 20, 2025

Submission Date

November 4, 2024

Acceptance Date

November 19, 2024

Published in Issue

Year 2025 Volume: 8 Number: 1

IZ

https://izlik.org/JA23WE67FN

Cite

RIS / Bibtex

APA

Azhar, W. A. A. W., Hasri, H., Tan, S. Y., Subramanie, D., Musa, A., Zahedi, F. D., Wahid, S. T. A., Karim, N. A., Shah, S. A., Hamizan, A. W., & Husain, S. (2025). The Effect of Vitamin C on Histamine and Dust Mite Epicutaneous Test Responses: A Randomized Control Trial. European Journal of Rhinology and Allergy, 8(1), 6-11. https://izlik.org/JA23WE67FN

AMA

1.Azhar WAAW, Hasri H, Tan SY, et al. The Effect of Vitamin C on Histamine and Dust Mite Epicutaneous Test Responses: A Randomized Control Trial. Eur J Rhinol Allergy. 2025;8(1):6-11. https://izlik.org/JA23WE67FN

Chicago

Azhar, Wan Abdul Aziz Wan, Husna Hasri, Sze Ying Tan, et al. 2025. “The Effect of Vitamin C on Histamine and Dust Mite Epicutaneous Test Responses: A Randomized Control Trial”. European Journal of Rhinology and Allergy 8 (1): 6-11. https://izlik.org/JA23WE67FN.

EndNote

Azhar WAAW, Hasri H, Tan SY, Subramanie D, Musa A, Zahedi FD, Wahid STA, Karim NA, Shah SA, Hamizan AW, Husain S (April 1, 2025) The Effect of Vitamin C on Histamine and Dust Mite Epicutaneous Test Responses: A Randomized Control Trial. European Journal of Rhinology and Allergy 8 1 6–11.

IEEE

[1]W. A. A. W. Azhar et al., “The Effect of Vitamin C on Histamine and Dust Mite Epicutaneous Test Responses: A Randomized Control Trial”, Eur J Rhinol Allergy, vol. 8, no. 1, pp. 6–11, Apr. 2025, [Online]. Available: https://izlik.org/JA23WE67FN

ISNAD

Azhar, Wan Abdul Aziz Wan - Hasri, Husna - Tan, Sze Ying - Subramanie, Divaharan - Musa, Anasuhah - Zahedi, Farah Dayana - Wahid, Samirah Taufiq Abdul et al. “The Effect of Vitamin C on Histamine and Dust Mite Epicutaneous Test Responses: A Randomized Control Trial”. European Journal of Rhinology and Allergy 8/1 (April 1, 2025): 6-11. https://izlik.org/JA23WE67FN.

JAMA

1.Azhar WAAW, Hasri H, Tan SY, Subramanie D, Musa A, Zahedi FD, Wahid STA, Karim NA, Shah SA, Hamizan AW, Husain S. The Effect of Vitamin C on Histamine and Dust Mite Epicutaneous Test Responses: A Randomized Control Trial. Eur J Rhinol Allergy. 2025;8:6–11.

MLA

Azhar, Wan Abdul Aziz Wan, et al. “The Effect of Vitamin C on Histamine and Dust Mite Epicutaneous Test Responses: A Randomized Control Trial”. European Journal of Rhinology and Allergy, vol. 8, no. 1, Apr. 2025, pp. 6-11, https://izlik.org/JA23WE67FN.

Vancouver

1.Wan Abdul Aziz Wan Azhar, Husna Hasri, Sze Ying Tan, Divaharan Subramanie, Anasuhah Musa, Farah Dayana Zahedi, Samirah Taufiq Abdul Wahid, Norwahidah Abdul Karim, Shamsul Azhar Shah, Aneeza W Hamizan, Salina Husain. The Effect of Vitamin C on Histamine and Dust Mite Epicutaneous Test Responses: A Randomized Control Trial. Eur J Rhinol Allergy [Internet]. 2025 Apr. 1;8(1):6-11. Available from: https://izlik.org/JA23WE67FN