Effect of Staphylococcus Aureus on the NLRP3 Inflammasome, Caspase-1 and IL-1 β Expression in the Nasal Epithelial Cells in Chronic Rhinosinusitis

Amanj Saber; Rashida Hussain; Sravya Nakka; Svante Hugosson

doi:10.5152/ejra.2019.110

Effect of Staphylococcus Aureus on the NLRP3 Inflammasome, Caspase-1 and IL-1 β Expression in the Nasal Epithelial Cells in Chronic Rhinosinusitis

Abstract

Objective: This study aimed to elucidate the effect of Staphylococcus aureus and budesonide on the mRNA expression and the biologic role (caspase-1 activation and IL-1β secretion) of NLRP3 inflammasome in primary nasal epithelial cells (NECs) in patients with chronic rhinosinusitis (CRS) and healthy controls. Material and Methods: Brush biopsies isolated from both patients and healthy controls were denoted respectively for our experiments. These were treated with S. aureus strains (four strains) only and in combination with budesonide (0, 10, 100, 1000 nM). NECs treated with only budesonide (0, 10, 100, 1000 nM) and untreated NECs were used as controls. Expression of NLRP3, caspase-1, IL-1β along with NLRC1/2 was analyzed by qPCR. Caspase-1 activity was measured by fluorogenic substrates Ac-YVAD-AMC. Enzyme-linked immunosorbent (ELISA) assay was performed to measure IL-1β production. Results: The mRNA levels of NLRC1, NLRC2, caspase-1, and IL-1β significantly increased, while NLRP3 demonstrated a trend toward elevation in the CRS group compared to the healthy controls. Infection with S. aureus increased caspase-1 activity and IL-1β secretion. However, treatment with budesonide decreased mRNA expression of NLRC2 and IL-1β secretion. Conclusion: Increase in the caspase-1 activity and IL-1β levels, due to possible activation of NLRP3 inflammasomes, upon S. aureus infection, may have an important role in the pathogenesis of CRS.

Keywords

References

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Details

Primary Language

English

Subjects

Otorhinolaryngology

Journal Section

Research Article

Authors

Amanj Saber This is me
0000-0002-4141-4256
Sweden

Rashida Hussain This is me
0000-0003-4946-3228
Sweden

Sravya Nakka This is me
0000-0002-1279-8238
Sweden

Svante Hugosson This is me
0000-0003-4194-9346
Sweden

Publication Date

May 29, 2019

Submission Date

February 11, 2019

Acceptance Date

May 2, 2019

Published in Issue

Year 2019 Volume: 2 Number: 1

DOI

https://doi.org/10.5152/ejra.2019.110

IZ

https://izlik.org/JA33RK83HR

Cite

RIS / Bibtex

APA

Saber, A., Hussain, R., Nakka, S., & Hugosson, S. (2019). Effect of Staphylococcus Aureus on the NLRP3 Inflammasome, Caspase-1 and IL-1 β Expression in the Nasal Epithelial Cells in Chronic Rhinosinusitis. European Journal of Rhinology and Allergy, 2(1), 6-12. https://doi.org/10.5152/ejra.2019.110

AMA

1.Saber A, Hussain R, Nakka S, Hugosson S. Effect of Staphylococcus Aureus on the NLRP3 Inflammasome, Caspase-1 and IL-1 β Expression in the Nasal Epithelial Cells in Chronic Rhinosinusitis. Eur J Rhinol Allergy. 2019;2(1):6-12. doi:10.5152/ejra.2019.110

Chicago

Saber, Amanj, Rashida Hussain, Sravya Nakka, and Svante Hugosson. 2019. “Effect of Staphylococcus Aureus on the NLRP3 Inflammasome, Caspase-1 and IL-1 β Expression in the Nasal Epithelial Cells in Chronic Rhinosinusitis”. European Journal of Rhinology and Allergy 2 (1): 6-12. https://doi.org/10.5152/ejra.2019.110.

EndNote

Saber A, Hussain R, Nakka S, Hugosson S (May 1, 2019) Effect of Staphylococcus Aureus on the NLRP3 Inflammasome, Caspase-1 and IL-1 β Expression in the Nasal Epithelial Cells in Chronic Rhinosinusitis. European Journal of Rhinology and Allergy 2 1 6–12.

IEEE

[1]A. Saber, R. Hussain, S. Nakka, and S. Hugosson, “Effect of Staphylococcus Aureus on the NLRP3 Inflammasome, Caspase-1 and IL-1 β Expression in the Nasal Epithelial Cells in Chronic Rhinosinusitis”, Eur J Rhinol Allergy, vol. 2, no. 1, pp. 6–12, May 2019, doi: 10.5152/ejra.2019.110.

ISNAD

Saber, Amanj - Hussain, Rashida - Nakka, Sravya - Hugosson, Svante. “Effect of Staphylococcus Aureus on the NLRP3 Inflammasome, Caspase-1 and IL-1 β Expression in the Nasal Epithelial Cells in Chronic Rhinosinusitis”. European Journal of Rhinology and Allergy 2/1 (May 1, 2019): 6-12. https://doi.org/10.5152/ejra.2019.110.

JAMA

1.Saber A, Hussain R, Nakka S, Hugosson S. Effect of Staphylococcus Aureus on the NLRP3 Inflammasome, Caspase-1 and IL-1 β Expression in the Nasal Epithelial Cells in Chronic Rhinosinusitis. Eur J Rhinol Allergy. 2019;2:6–12.

MLA

Saber, Amanj, et al. “Effect of Staphylococcus Aureus on the NLRP3 Inflammasome, Caspase-1 and IL-1 β Expression in the Nasal Epithelial Cells in Chronic Rhinosinusitis”. European Journal of Rhinology and Allergy, vol. 2, no. 1, May 2019, pp. 6-12, doi:10.5152/ejra.2019.110.

Vancouver

1.Amanj Saber, Rashida Hussain, Sravya Nakka, Svante Hugosson. Effect of Staphylococcus Aureus on the NLRP3 Inflammasome, Caspase-1 and IL-1 β Expression in the Nasal Epithelial Cells in Chronic Rhinosinusitis. Eur J Rhinol Allergy. 2019 May 1;2(1):6-12. doi:10.5152/ejra.2019.110