Microalgal biovolume and taxonomic composition along a restoration chronosequence in sandy post-mining soils

Oleksandr Bren; Oksana Bren; Maria čížková; Anastasiia Kolomiiets; Jana Kvíderová; Sergey Podorozhny; Milada Vítová

doi:10.18393/ejss.1934414

Microalgal biovolume and taxonomic composition along a restoration chronosequence in sandy post-mining soils

Abstract

This study examined microalgal biovolume and taxonomic composition across a restoration chronosequence in sandy post-mining soils and their relationships with abiotic soil properties. The research was conducted in a single sand pit in Central Europe, representing a case study of a typical regional post-mining environment. Samples were collected in winter, spring, and summer from nine experimental plots representing a 4–43-year chronosequence after mining cessation, and from three control plots where mining did not occur. The microalgal biovolume was higher in recently restored plots and lower in older plots. Although biovolume varied seasonally, seasonal changes in taxonomic composition were limited. Biovolume showed a significant negative correlation with organic matter content in all studied seasons, and a similar pattern was observed for Oscillatoriales and Zygnemales, whereas correlations for other taxa varied among seasons and were not consistently significant. In this study, microalgal characteristics did not provide sufficient information for assessing the restoration of sandy post-mining soils under field conditions. However, the decrease in biovolume along the restoration chronosequence, its negative relationship with organic matter content, and the similar relationships observed for taxa identified in this study suggest directions for further investigation. These findings require verification across multiple post-mining sites.

Keywords

Supporting Institution

Technology Agency of the Czech Republic and the Ministry of the Environment

Project Number

SS0702006

Thanks

We gratefully acknowledge the assistance provided by Kamila Botková, Pavel Klimeš, and Tereza Brzobohatá (Heidelberg Materials) in the implementation of this study. The study was conducted within the framework of The Quarry Life Award – 6th Edition (Heidelberg Materials) at the Institute of Botany of the Czech Academy of Sciences (RVO67985939). The work was co-financed by the Technology Agency of the Czech Republic and the Ministry of the Environment from the state budget under the Program Environment for Life (SS0702006) in the framework of the National Recovery Plan from the European Recovery and Resilience Instrument. Oleksandr Bren also received support from the Researchers at Risk-2024 Fellowship (Czech Academy of Sciences) and the IIE Scholar Rescue Fund (IIE-SRF) Fellowship.

References

Abinandan, S., Subashchandrabose, S.R., Venkateswarlu, K., Megharaj, M., 2019. Soil microalgae and cyanobacteria: Biotechnological potential in the maintenance of soil fertility and health. Critical Reviews in Biotechnology 39: 981–998.
Andreieva, V.M., 1998. Soil and aerophilic green algae (Chlorophyta: Tetrasporales, Chlorococcales, Chlorosarcinales). Nauka, Moscow. 351p. [in Russian]
Bateman, A., Muñoz-Rojas, M., 2019. To whom the burden of soil degradation and management concerns. In: Advances in Chemical Pollution, Environmental Management and Protection, Volume 4, pp.1–22.
Belnap, J., Büdel, B., Lange, O., 2001. Biological Soil Crusts: Characteristics and Distribution. In: Biological Soil Crusts: Structure, Function, and Management. Belnap, J., Lange, O.L. (Eds.). Vol 150, Springer-Verlag Berlin Heidelberg, pp. 3-30.
Ettl, H., Gärtner, G., 1988. Süßwasserflora von Mitteleuropa 10.: Chlorophyta II. Tetrasporales, Chlorococcales, Gloeodendrales.Fischer Verlag. Stuttgart. 437 p. [in German]
Frindte, K., Pape, R., Werner, K., Löffler, J., Knief, C., 2019. Temperature and soil moisture control microbial community composition in an arctic–alpine ecosystem along elevational and micro-topographic gradients. The ISME Journal 13(8): 2031–2043.
Ghanei-Bafghi, M.-J., Feiznia, S., Mokhtari, A.R., Jaafari, M., Tavili, A., Khodaeian, Z., 2023. Agricultural soil contamination and degradation near a mining area in an arid region. Journal of Geochemical Exploration 256: 107349.
Hammer, Ø., Harper, D., Ryan, P., 2001. PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4: 1–9.

Hillebrand, H., Dürselen, C.D., Kirschtel, D., Pollingher, U., Zohary, T., 1999. Biovolume calculation for pelagic and benthic microalgae. Journal of Phycology 35: 403–424.
Hu, C.-X., Liu, Y.-D., 2003. Primary succession of algal community structure in desert soil. Acta Botanica Sinica 45: 917–924.
Huang, L.-N., Tang, F.-Z., Song, Y.-S., Wan, C.-Y., Wang, S.-L., Liu, W.-Q., Shu, W.-S., 2011. Biodiversity, abundance, and activity of nitrogen-fixing bacteria during primary succession on a copper mine tailings. FEMS Microbiology Ecology 78: 439–450.
ISO 10390:2021. Soil, treated biowaste and sludge – Determination of pH. Available at [Access date: 20.01.2026]: https://www.iso.org/standard/75243.html
ISO 10523:2008. Water quality — Determination of pH. Available at [Access date: 20.01.2026]: https://www.iso.org/standard/51994.html
ISO 11465:2025. Sludge and solid environmental matrices — Determination of dry residue or water content and calculation of the dry matter fraction on a mass basis. Available at [Access date: 20.01.2026]: https://www.iso.org/standard/88192.html
ISO 7888:1985. Water quality — Determination of electrical conductivity. Available at [Access date: 20.01.2026]: https://www.iso.org/standard/14838.html
Jelecevic, A., Sager, M., Vollprecht, D., Puschenreiter, M., Liebhard, P., 2021. Partitioning of heavy metals in different particle-size fractions of soils from former mining and smelting locations in Austria. Eurasian Journal of Soil Science 10(2): 123 - 131.
Johnson, S., Kuske, C., Carney, T., Housman, D., Gallegos-Graves, L., Belnap, J., 2012. Increased temperature and altered summer precipitation have differential effects on biological soil crusts in a dryland ecosystem. Global Change Biology 18: 2583-2593.
Joseph, J., Ray, J.G., 2024. A critical review of soil algae as a crucial soil biological component of high ecological and economic significance. Journal of Phycology 60: 229-253.
Kaštovská, K., Elster, J., Stibal, M., Šantrůčková, H., 2005. Microbial assemblages in soil microbial succession after glacial retreat in Svalbard (High Arctic). Microbial Ecology 50: 396–407.
Komárek, J., 2013. Cyanoprokaryota 3. Heterocytous genera. Süßwasserflora von Mitteleuropa, Vol. 19/3. Springer Spektrum, Berlin, Heidelberg. 1130p.
Komárek, J., Anagnostidis, K., 1998. Cyanoprokaryota 1. Chroococcales. Süßwasserflora von Mitteleuropa, Vol. 19/1. Gustav Fischer Verlag, Stuttgart–Jena. 548p.
Komárek, J., Anagnostidis, K., 2005. Cyanoprokaryota 2. Oscillatoriales. Süßwasserflora von Mitteleuropa, Vol. 19/2. Elsevier Spectrum, Amsterdam. 759p.
Krammer, K., Lange-Bertalot, H., 1986–2004. Bacillariophyceae. Parts 1–4. Gustav Fischer Verlag, Stuttgart.
Kwak, J. I., Nam, S.-H., Kim, S. W., Bajagain, R., Jeong, S.-W., An, Y.-J., 2019. Changes in soil properties after remediation influence the performance and survival of soil algae and earthworm. Ecotoxicology and Environmental Safety 174: 189-196.
Langhans, T., Storm, C., Schwabe, A., 2009. Community assembly of biological soil crusts of different successional stages in a temperate sand ecosystem, as assessed by direct determination and enrichment techniques. Microbial Ecology 58: 394–407.
Lukešová, A., 2001. Soil algae in brown coal and lignite post-mining areas in Central Europe (Czech Republic and Germany). Restoration Ecology 9: 341–350.
Lukešová, A., Komárek, J., 1987. Succession of soil algae on dumps from strip coal-mining in the Most Region (Czechoslovakia). Folia Geobotanica et Phytotaxonomica 22: 355–362.
Muñoz-Rojas, M., Román, R., Roncero Ramos, B., Erickson, T., Merritt, D., Aguila-Carricondo, P., Cantón, Y., 2018. Cyanobacteria inoculation enhances carbon sequestration in soil substrates used in dryland restoration. Science of The Total Environment 636: 1149–1154.
Oksanen, J., Blanchet, F.G., Kindt, R., Legendre, P., Minchin, P.R., O’Hara, R.B., Simpson, G.L., Solymos, P., Stevens, M.H.H., Wagner, H., 2015. Vegan: Community Ecology Package. R package version 2.2-1.
Pedrinho, A., Mendes, L., Pereira, A., Araujo, A., Vaishnav, A., Karpouzas, D., Singh, B., 2024. Soil microbial diversity plays an important role in resisting and restoring degraded ecosystems. Plant and Soil 500: 325–349.
R Core Team. 2025. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing Vienna, Austria. Available at [Access date: 20.01.2026]: https://www.r-project.org/
Rahmonov, O., Cabala, J., Bednarek, R., Rożek, D., Florkiewicz, A., 2015. Role of soil algae on the initial stages of soil formation in sandy polluted areas. Ecological Chemistry and Engineering S 22: 675–690.
Robinson, J.M., Liddicoat, C., Muñoz-Rojas, M., Breed, M.F., 2024. Restoring soil biodiversity. Current Biology 34: R393–R398
Schulz, K., Mikhailyuk, T., Dreßler, M., Leinweber, P., Karsten, Ulf., 2016. Biological Soil Crusts from Coastal Dunes at the Baltic Sea: Cyanobacterial and Algal Biodiversity and Related Soil Properties. Microbial Ecology 71: 178-193.
Stephan, K., Hubbart, J., 2022. Plant community, soil and microclimate attributes after 70 years of natural recovery of an abandoned limestone quarry. Land 12(1): 117.
Tarchevsky, V.V., Shtina, E.A., 1967. Development of algae on industrial dumps. In: Proceedings of the Interuniversity Conference. Kirov, pp. 146–150.
Wang, Z., Wang, G., Ren, T., Wang, H., Xu, Q., Zhang, G., 2021. Assessment of soil fertility degradation affected by mining disturbance and land use in a coalfield via machine learning. Ecological Indicators 125: 107608.
Wong, S.Y., Machado-de-Lima, N., Wilkins, D., Zhang, E., Ferrari, B.C., 2025. Fine-scale landscape heterogeneity drives microbial community structure at Robinson Ridge, East Antarctica. Science of the Total Environment 958: 177964.
Zhang X., Koehler, H. 2022. Soil algae for combating soil degradation – greenhouse experiment with different soil amendments. Soil Research 61: 70–82.
Zhang, B., Zhang, Y., Downing, A., Niu, Y., 2011. Distribution and composition of Cyanobacteria and microalgae associated with biological soil crusts in the Gurbantunggut desert, China. Arid Land Research and Management 25: 275-293.

Details

Primary Language

English

Subjects

Soil Sciences and Plant Nutrition (Other)

Journal Section

Research Article

Authors

Oleksandr Bren ^* This is me
0000-0001-7423-9258
Czech Republic

Oksana Bren This is me
0009-0003-7632-6285
Ukraine

Maria čížková This is me
0000-0002-2328-8583
Czech Republic

Anastasiia Kolomiiets This is me
0000-0001-7849-1135
Czech Republic

Jana Kvíderová This is me
0000-0002-5700-477X
Czech Republic

Sergey Podorozhny This is me
0000-0002-7702-7602
Ukraine

Milada Vítová This is me
0000-0002-0552-3295
Czech Republic

Publication Date

June 30, 2026

Submission Date

January 20, 2026

Acceptance Date

April 13, 2026

Published in Issue

Year 2026 Volume: 15 Number: 3

DOI

https://doi.org/10.18393/ejss.1934414

IZ

https://izlik.org/JA72LS79RP

Cite

RIS / Bibtex

APA

Bren, O., Bren, O., čížková, M., Kolomiiets, A., Kvíderová, J., Podorozhny, S., & Vítová, M. (2026). Microalgal biovolume and taxonomic composition along a restoration chronosequence in sandy post-mining soils. Eurasian Journal of Soil Science, 15(3), 375-386. https://doi.org/10.18393/ejss.1934414

AMA

1.Bren O, Bren O, čížková M, et al. Microalgal biovolume and taxonomic composition along a restoration chronosequence in sandy post-mining soils. EJSS. 2026;15(3):375-386. doi:10.18393/ejss.1934414

Chicago

Bren, Oleksandr, Oksana Bren, Maria čížková, et al. 2026. “Microalgal Biovolume and Taxonomic Composition Along a Restoration Chronosequence in Sandy Post-Mining Soils”. Eurasian Journal of Soil Science 15 (3): 375-86. https://doi.org/10.18393/ejss.1934414.

EndNote

Bren O, Bren O, čížková M, Kolomiiets A, Kvíderová J, Podorozhny S, Vítová M (June 1, 2026) Microalgal biovolume and taxonomic composition along a restoration chronosequence in sandy post-mining soils. Eurasian Journal of Soil Science 15 3 375–386.

IEEE

[1]O. Bren et al., “Microalgal biovolume and taxonomic composition along a restoration chronosequence in sandy post-mining soils”, EJSS, vol. 15, no. 3, pp. 375–386, June 2026, doi: 10.18393/ejss.1934414.

ISNAD

Bren, Oleksandr - Bren, Oksana - čížková, Maria - Kolomiiets, Anastasiia - Kvíderová, Jana - Podorozhny, Sergey - Vítová, Milada. “Microalgal Biovolume and Taxonomic Composition Along a Restoration Chronosequence in Sandy Post-Mining Soils”. Eurasian Journal of Soil Science 15/3 (June 1, 2026): 375-386. https://doi.org/10.18393/ejss.1934414.

JAMA

1.Bren O, Bren O, čížková M, Kolomiiets A, Kvíderová J, Podorozhny S, Vítová M. Microalgal biovolume and taxonomic composition along a restoration chronosequence in sandy post-mining soils. EJSS. 2026;15:375–386.

MLA

Bren, Oleksandr, et al. “Microalgal Biovolume and Taxonomic Composition Along a Restoration Chronosequence in Sandy Post-Mining Soils”. Eurasian Journal of Soil Science, vol. 15, no. 3, June 2026, pp. 375-86, doi:10.18393/ejss.1934414.

Vancouver

1.Oleksandr Bren, Oksana Bren, Maria čížková, Anastasiia Kolomiiets, Jana Kvíderová, Sergey Podorozhny, Milada Vítová. Microalgal biovolume and taxonomic composition along a restoration chronosequence in sandy post-mining soils. EJSS. 2026 Jun. 1;15(3):375-86. doi:10.18393/ejss.1934414