Effect of salt stress on concentration of nitrogen and phosphorus in root and leaf of strawberry plant

Abstract

In this study the effect of salt stress on the concentrations of nitrogen (N) and phosphorus (P) in the leaves and the roots of two strawberry (Fragaria vesca L.) cultivars (Camarosa and Sweet Charlie) was investigated on cold stored bare-rooted seedlings grown in buckets filled with coarse sand. The treatments consisting of no-NaCl control, 1760, 2400, and 3040 mg L^-1 of NaCl in half-strength Hoagland nutrient solution were applied to the plants for six months. During the experiment, leaf and root sampling were performed two times with five months interval. Roots and leaves of the plants were analyzed for Na, Cl, N and P. Analysis of variance (ANOVA) procedures was performed in Three Factors Completely Randomized Design for plant analysis results. Additionally orthogonal comparison was applied to the significant salinity effects. Cultivar and sampling time affected N, P, Na and Cl concentrations of the roots significantly. Cultivar-sampling time and sampling time-salinity interactions were significant for the N, P and Na concentrations of the roots. Salinity solely affected Cl concentrations of the roots significantly. All the treatments affected the concentrations of P, Na and Cl of the leaves significantly. The N concentrations of the leaves were affected significantly by only sampling time. Cultivar-salinity and sampling time-salinity interactions were found significant in the leaf N concentrations of the plants. The results show that the cultivars probably have different strategies in arrangement of N and P composition under salinity.

Keywords

• NaCl salinity,Fragaria vesca L.,Camaraso,Sweet Charlie,mineral nutrients

References

1. Awang, Y.B., Atherton, J.G., Taylor, A.J., 1993a. Salinity effects on strawberry plants grown in rockwool. I. Growth and leaf water relations. Journal of Horticultural Science 68(5): 783-790.
2. Awang, Y.B., Atherton, J.G., Taylor, A.J., 1993b. Salinity effects on strawberry plants grown in rockwool. I. Fruit quality. Journal of Horticultural Science 68(5): 791-795.
3. Botella, M.A., Cerdá, A., Lips, S.H., 1994. Kinetics of NO3- and NH4+ uptake by wheat seedlings. Effect of salinity and nitrogen source. Journal of Plant Physiology 144(1): 53-57.
4. Botella, M.A., Martínez, V., Nieves, M., Cerdá, A., 1997. Effect of salinity on the growth and nitrogen uptake by wheat seedlings. Journal of Plant Nutrition 20(6): 793-804.
5. Brown, J.G., Jackson, R.K., 1955. A note on the potentiometric determination of chloride. Proceedings of the American Society for Horticultural Science 65: 187-193.
6. Cangi, R., Tarakcioglu, C., 2006. Effects of sodium chloride ions on growth and mineral contents of kiwifruit plants. Asian Journal of Chemistry 18(3): 1871-1878
7. Carter, D.L., 1981. Salinity and plant productivity. In: Handbook series in nutrition and food. Chemical Rubber Co., Cleveland, OH, USA. pp.146-151.
8. Cerda, A., Bingham, F.T., Hoffman, G., 1977. Interactive effect of salinity and phosphorus on sesame. Soil Science Society of America Journal 41(5): 915-918.

9. Demiral, M.A., 2005. Comparative response of two olive (Olea europaea L.) cultivars to salinity. Turkish Journal of Agriculture and Forestry 29(4): 267-274.
10. Demiral, M.A., Aydin, M., Yorulmaz, A., 2005. Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. Turkish Journal of Biology 29(2): 117-123.
11. Dunlop, J., Phung, H.T., Meeking, R., White, D.W.R., 1997. The kinetics associated with phosphate absorption by Arabidopsis and its regulation by phosphorus status. Australian Journal of Plant Physiology 24(5): 623-629.
12. Durand, M., Lacan, D., 1994. Sodium partitioning within the shoot of soybean. Physiologia Plantarum 91(1): 65-71.
13. Furihata, T., Suzuki, M., Sakurai, H., 1992. Kinetic characterization of two phosphate uptake systems with different affinities in suspension-cultured Catharanthus roseus protoplasts. Plant and Cell Physiology 33(8): 1151-1157.
14. Ghafoor, A., Qadir, M., Murtaza, G., 2004. Salt-affected soils: Principles of management. Allied Book Centre Publications, Lahore, Pakistan. pp. 110-123.
15. Gorham, J., 1992. Salt tolerance of plants. Science Progress 76(3-4): 273-285.
16. Grieve, C.M., Wang, D., Shannon, M.C., 2003. Salinity and irrigation method affect mineral ion relations of soybean. Journal of Plant Nutrition 26(4): 901-913.
17. Hoagland, D.R., Arnon, D.I.,1950. The water-culture method for growing plants without soil. University of California, College of Agriculture, California Agricultural Experiment Station, Circular No. 347. Berkeley, USA. 39p.
18. Jacoby, B., 1979. Sodium recirculation and loss from Phaseolus vulgaris L. Annals of Botany 43(6): 741-744.
19. Kalifa, A., Barthakur, N.N., Donnelly, D.J., 2000. Phosphorus reduces salinity stress in micropropated potato. American Journal of Potato Research 77(3): 179-182.
20. Kasırğa, E., Demiral, M.A., 2016. Salt stress-mineral nutrient relations in olive (Olea europaea L.) plant . Eurasian Journal of Soil Science 5 (4): 307- 313.
21. Keutgen, A.J., Pawelzik, E., 2009. Impacts of NaCl stress on plant growth and mineral nutrient assimilation in two cultivars of strawberry. Environmental and Experimental Botany 65(2-3): 170-176.
22. Köhler, B., Raschke, K., 2000. The delivery of salts to the xylem. Three types of anion conductance in the plasmalemma of the xylem parenchyma of roots of barey. Plant Physiology 122(1): 243-254.
23. Larson, K.D., 1994. Strawberry, In: Handbook of environmental physiology of fruit crops. Vol. 1. Temperate crops. Schaffer, B., Anderson, P.C. (Eds.). CRC Press, Boca Raton, USA. pp. 271–297.
24. Leggewie, G., Wilmitzer, L., Riesmeier, J.W., 1997. Two cDNAs from potato are able to complement a phosphate uptake-deficient yeast mutant: identification of phosphate transporters from higher plants. The Plant Cell 9(3): 381-632.
25. Lieten, F., 1997. Chloride nutrition of strawberries grown on peat bags. Journal of Small Fruit & Viticulture 5(1): 51-61.
26. Little, T.M., Hills, F.J., 1978. Agricultural experimentation: design and analysis. John Wiley and Sons Inc. New York, USA, 350p.
27. Loupassaki, M.H., Chartzoulakis, K.S., Digalaki, N.B., Androulakis I.I., 2002. Effects of salt stress on concentration of nitrogen, phosphorus, potassium, calcium, magnesium and sodium in leaves, shoots and roots of six olive cultivars. Journal of Plant Nutrition 25(11): 2457-2482.
28. Martinez, V., Läuchli, A., 1994. Salt-induced inhibition of phosphate uptake in plants of cotton (Gossypium hirsutum L.). New Phytologist 126(4): 609-614.
29. Marschner, H., 1995. Mineral nutrition of higher plants. 2nd Edition. Academic Press, London. UK. 901p.
30. Munns, R., 2002. Comparative physiology of salt and water stress. Plant, Cell and Environment 25(2): 239-250.
31. Navarro, J.M., Botella, M.A., Cerdá, A., Martinez, V., 2001. Phosphorus uptake and translocation in salt-stressed melon plants. Journal of Plant Physiology 158(3): 375-381.
32. Orcutt, D.M., Nielsen, E.T., 2000. The physiology of plants under stress: Soil and Biotic Factors. John Wiley and Sons Inc. New York, USA. 696p.
33. Roberts, J.K.M., Linker, C.S., Benoit, A.G., Jardetzky, O., Nieman, R.H., 1984. Salt stimulation of phosphate uptake in maize root tips studied by 31P nuclear magnetic resonance. Plant Physiology 75(4): 947-950.
34. Rubinigg, M., Posthumus, F., Ferschke, M., Elzenga, J.T.M., Stulen, I., 2003. Effects of NaCl salinity on 15N-nitrate fluxes and specific root length in the halophyte Plantago maritima L. Plant and Soil 250(2): 201-213.
35. Schachtman, P., Reid, R.J., Ayling, S.M., 1998. Phosphorus uptake by plants: from soil to cell. Plant Physiology 116(2): 447-453.
36. Shibli, R.A., Sawwan, J., Swaidat, I., Tahat, M., 2001. Increased phosphorus mitigates the adverse effects of salinity in tissue culture. Communication in Soil Science and Plant Analysis 32(3-4): 429-440.
37. Storey, R., Wyn-Jones, R.G., 1977. Quarternary ammonium compounds in plants in relation to salt resistance. Phytochemistry 16(4): 447-453.
38. Ulrich, A., Mostafa, M.A.E., Allen, W.W., 1980. Strawberry deficiency symptoms: A visual and plant analysis guide to fertilization. University of California, Division of Agricultural Sciences, Publication No. 4098, California, USA. 58p.
39. Westerman, R.L., 1990. Soil testing and plant analysis. Soil Science Society of America (SSAA) Book Series, Vol. 3, Issue 3. SSSA Publications, Madison, Wisconsin, USA. 784p.