Screening for Lotus creticus growth promoting rhizobacteria under greenhouse conditions

Year 2018, Volume: 7 Issue: 4, 284 - 291, 01.10.2018

İmane Achkouk Saida Aarab Amin Laglaoui Mohammed Bakkali Abdelhay Arakrak

https://doi.org/10.18393/ejss.436181

Cited By: 1

Abstract

Utilization
of plant growth promoting rhizobacteria (PGPR) is now gradually increasing in
agriculture and offers an attractive way to replace chemical fertilizers,
pesticides, and supplements. This study was conducted with a view to isolate
bacteria from the rhizosphere of the legume Lotus
creticus (L. creticus) and to
assess their plant growth promoting functional potentialities. A total of 113
rhizobacteria was isolated from the rhizosphere of L. creticus and were tested for their capacity of solubilizing
tricalcium phosphate (TCP) on Pikovskaya (PVK) solid medium. Out of 29
phosphate solubilizing bacteria (PSB), 5 isolates were selected for their
solubilization diameters (between 0.6 and 1.5 cm). These isolates were
characterized for plant growth promoting (PGP) traits. The results showed that
the highest concentration of indole acetic acid (IAA) was produced by LCR33
(19.08 ± 0.96 mg L^-1). All 5 isolates could produce hydrogen cyanide
(HCN), siderophores, ammonia and amino-cyclopropane carboxylate (ACC) deaminase.
The isolates were evaluated for TCP solubilizing quantitative assay in PVK
liquid medium. The concentrations of solubilized P were between 43.34±0.18 mg L^-1
and 173.57±0.77 mg L^-1. This solubilization was accompanied by a pH
decrease of the culture media from 7 to 4.06. Furthermore, the 5 selected PSB
were tested in vitro for antagonism against phytopathogenic fungus Fusarium oxysporum. In fact, all the
PSB, were capable of inhibiting its growth and the highest percentages of
inhibition were obtained for LCP27 and LCR33 (48.15±0.99% and 40.74±0.45%).
Also, the effect of these 2 PSB on growth of L. creticus plants was investigated under greenhouse conditions.
Significant increases were obtained for shoot and root length and dry and fresh
matter production of plants as compared to the uninoculated control. These PSB
could be recommended as biofertilizers for contributing to the rehabilitation
of degraded soils.

Keywords

Farmyard manure, compost, soil acidity, incubation

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