Field suppression of Fusarium wilt and microbial population Shifts in tomato rhizosphere following soil treatment with two selected endophytic bacteria

Year 2019, Volume: 8 Issue: 3, 208 - 220, 01.07.2019

Rania Aydi Ben Abdallah Hayfa Jabnoun-khiareddine Ahlem Nefzi Fakher Ayed Mejda Daami-remadi

https://doi.org/10.18393/ejss.556780

Cited By: 3

Abstract

Two
endophytic bacteria, Bacillus subtilis SV41 (KR818071) and B.
amyloliquefaciens subsp.
plantarum SV65 (KR818073), were assessed under field conditions for
their capacity to control tomato Fusarium wilt in tomato and their effects on
soil microbial activity. Six months after planting, Fusarium wilt severity,
estimated through the vascular browning extent in tomato stems, was
significantly reduced by 82.3 and 88.2% compared to control following bacterial treatments. The frequency
of F.oxysporum re-isolation from roots, collars and stems was also
significantly lowered in treated plants compared to controls. These effects
were associated with a significant improvement, by 10.6 to 16.3%over control,
in plant height and root fresh weight and an increase in fruit production by
8.4-12.5%. As for microbial activity, F. oxysporum population in the
rhizosphere of tomato plants treated with B. subtilis SV41 and B.
amyloliquefaciens subsp. plantarum SV65 was reduced by
87.5-91.7%compared to the initial soil (sampled before planting) and by
88.4-92.3% relative to the rhizospheric soil of untreated plants (control
soil). A significant enhancement in the total culturable bacterial community
was also noted in the rhizosphere of tomato plants treated with both strains compared to initial and
control soils where a significant enrichment in Pseudomonas and
actinobacteria community was recorded.

Keywords

Endophytic bacteria, Fusarium wilt, growth-promoting, microbial community, soil, tomato

References

• Aydi Ben Abdallah, R., Jabnoun-Khiareddine, H., Mokni-Tlili, S., Nefzi, A., Medimagh-Saidana, S., Daami-Remadi, M., 2015. Soil-borne and Compost-borne Aspergillus Species for Biologically Controlling Post-harvest Diseases of Potatoes Incited by Fusarium sambucinum and Phytophthora erythroseptica. Journal of Plant Patholology and Microbiology 6(10): 324-330.
• Aydi Ben Abdallah, R., Jabnoun-Khiareddine, H., Nefzi, A., Mokni-Tlili, S., Daami-Remadi, M., 2016a. Biocontrol of Fusarium wilt and growth promotion of tomato plants using endophytic bacteria isolated from Solanum elaeagnifolium stems. Journal of Phytopathology 164(10): 811-824.
• Aydi Ben Abdallah, R., Jabnoun-Khiareddine, H., Nefzi, A., Mokni-Tlili, S., Daami-Remadi, M., 2016b. Endophytic bacteria from Datura metel for plant growth promotion and bioprotection against Fusarium wilt in tomato. Biocontrol Science and Technology 26(8): 1139-1165.
• Aydi Ben Abdallah, R., Stedel, C., Garagounis, C., Nefzi, A., Jabnoun-Khiareddine, H., Papadopoulou, K.P., Daami-Remadi, M., 2017. Involvement of lipopeptide antibiotics and chitinase genes and induction of host defense in suppression of Fusarium wilt by endophytic Bacillus spp. in tomato. Crop Protection 99:45-58.
• Barraquio, W.L., Revilla, L., Ladha, L.K., 1997. Isolation of endophytic diazotrophic bacteria from wetland rice. Plant and Soil 194(1-2):15-24.
• Bernard, E., Larkin, R.P., Tavantzis, S., Erich, M.S., Alyokhin, A., Gross, S.D., 2014. Rapeseed rotation, compost and biocontrol amendments reduce soilborne diseases and increase tuber yield in organic and conventional potato production systems. Plant and Soil 374(1-2): 611-627.
• Bibi, F., Yasir, M., Song, G.C., Lee, S.Y., Chung, Y.R., 2012. Diversity and characterization of endophytic bacteria associated with tidal flat plants and their antagonistic effects on Oomycetous plant pathogens. The Plant Pathology Journal 28(1): 20-31.
• Brzezinska, M.S., Jankiewicz, U., 2012. Production of antifungal chitinase by Aspergillus niger LOCK 62 and its potential role in the biological control. Current Microbiology 65(6): 666-672.
• Christina, A., Christapher, V., Bhore, S.J., 2013. Endophytic bacteria as a source of novel antibiotics: An overview. Pharmacognosy Reviews 7: 11-16.
• Compant, S., Reiter, B., Sessitsch, A., Nowak, J., Clément, C., Ait Barka, E., 2005. Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. strain PsJN. Applied and Environmental Microbiology 71: 1685-1693.
• Daami-Remadi, M., Souissi, A., Ben Oun, H., Mansour, M., Nasraoui, B., 2009. Salinity effects on Fusarium wilt severity and tomato growth. Dynamic Soil, Dynamic Plant 3: 61-69.
• Dalal, J., Kulkarni, N., 2013. Antagonistic and plant growth promoting potentials of indigenous endophytic bacteria of soybean (Glycine max (L) Merril). Current Research in Microbiology and Biotechnology 1(2): 62-69.
• Domenech, J., Reddy, M.S., Kloepper, J.W., Ramos, B., Gutierrez-Maňero, J., 2005. Combined application of the biological product LS213 with Bacillus, Pseudomonas or Chryseobacterium for growth promotion and biological control of soil-borne diseases in pepper and tomato. BioControl 51:245-258.
• Dong, H., Cohen, Y., 2002. Induced resistance in cotton seedlings against Fusarium wilt by dried biomass of Penicillium chrysogenum and its water extract. Phtoparasitica 30(1): 77-87.
• Dubey, R.K., Tripathi, V., Dubey, P.K., Singh, H.B., Abhilash, P.C., 2016. Exploring rhizospheric interactions for agricultural sustainability: the need of integrative research on multi-trophic interactions. Journal of Cleaner Production 115: 362-365.
• El-Tarabily, K.A., Sivasithamparam, K., 2006. Non-streptomycete actinomycetes as biocontrol agents of soil-borne fungal plant pathogens and as a plant growth-promoters. Soil Biology and Biohemistry 38(7): 1505-1520.
• El-Tarabily, KA., Soliman, M.H., Nassar, A.H., Al-Hassani, H.A., Sivasithamparam, K., McKenna, F., Hardy, G.E.St.J., 2000. Biological control of Sclerotinia minor using a chitinolytic bacterium and actinomycetes. Plant Pathology 49: 573-583.
• Etebarian, H.R., Scott, E.S., Wicks, T.J., 2003. Evaluation of Streptomyces strains as potential biological agents of Phytophthora erythroseptica. Iranian Journal of Plant Pathology 49: 49-63.
• Figueiredo, M.D.V.B., Bonifacio, A., Rodrigues, A.C., de Araujo, F.F., Stamford, N.P., 2016. Beneficial microorganisms: Current challenge to increase crop performance. In: Bioformulations: for Sustainable Agriculture, Arora et al., (Eds.). Springer India, pp. 53‒70.
• Glick, B.R., 2015. Beneficial Plant-Bacterial Interactions: Biocontrol mechanisms. Springer, Cham.
• Hallmann, J., Quadt-Hallmann, A., Mahaffee, W.F., Kloepper, J.W., 1997. Bacterial endophytes in agricultural crops. Canadian Journal of Microbiology 43(10): 895-914.
• Hwang, B.K., Ahn, S.J., Moon, S.S., 1994. Production, purification, and antifungal activity of the antibiotic nucleoside, tubercidin, produced by Streptomyces violaceoniger. Canadian Journal of Botany 72(4): 480-485.
• Idris, H.A., Labushagne, N., Korsten, L., 2007. Screening rhizobacteria for biological control of Fusarium root and crown rot of sorghum in Ethiopia. Biological Control 40(1): 97-106.
• Kalai-Grami, L., Saidi, S., Bachkouel, S., Ben Slimene, I., Mnari-Hattab, M., Hajlaoui, M.R., Limam, F., 2014. Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum. Applied Biochemistry and Biotechnology 174(1): 365-375.
• Kamara, V., Gangwar, M., 2015. Antifungal activity of actinomycets from rhizospheric soil of medicinal plants against phytopathogenic fungi. International Journal of Current Microbiologyand Applied Sciences 4(3): 182-187.
• Kettler, T.A., Doran, J.W., Gilbert, T.L., 2001. Simplified method for soil particle-size determination to accompany soil-quality analyses. Soil Science Society of American Journal 65(3): 849-852.
• Landa, B.B., Hervfis, A., Bettiol, W., Jimnez-Diaz, R.M., 1997. Antagonistic activity of Bacteria from the chickpea rhizosphere against Fusarium Oxysporum f. sp. Ciceris. Phytoparasitica 25(4): 305-318.
• Larkin, R.P., Tavantzis, S., 2013. Use of biocontrol organisms and compost amendments for improved control of soilborne diseases and increased potato production. American Journal of Potato Research 90(3): 261-270.
• Larkin, R.P., Honeycutt, C.W., 2006. Effects of different 3-year cropping systems on soil microbial communities and rhizoctonia diseases of potato. Phytopathology 96(1): 69-79.
• Larkin, R.P., Honeycutt, C.W., Griffin, T.S., 2006. Effect of swine and dairy manure amendments on microbial communities in three soils as influenced by environmental conditions. Biology and Fertility of Soils 43(1): 51-61.
• Ling, N., Xue, C., Huang, Q., Yang, X., Xu, Y., Shen, Q., 2010. Development of a mode of application of bioorganic fertilizer for improving the biocontrol efficacy of Fusarium wilt. Biological Control 55(5): 673-683.
• Mazzola, M., 2004. Assessment and management of soil community structure for disease suppression. Annual Review of Phytopathology 42: 35-59.
• Meena, S.K., Meena, V.S., 2017. Importance of soil microbes in nutrient use efficiency and sustainable food production. In: Agriculturally important microbes for sustainable agriculture: Application in crop production and protection, Meena, V.S., Mishra, P.K., Bisht, J.K., Pattanayak, A., (Eds.). Springer Nature Singapore, pp. 3-23.
• Mejdoub-Trabelsi, B., Aydi Ben Abdallah, R., Ammar, N., Daami-Remadi, M., 2017. Antifungal potential of extracellular metabolites from Penicillium pp. and Aspergillus spp. naturally associated to potato against Fusarium species causing tuber dry rot. Journal of Microbial and Biochemistry Technology 9(4): 181-190.
• Moretti, M., Gilardi, G., Gullino, M.L., Garibaldi, A., 2008. Biological control potential of Achromobacter xylosoxydans for suppressing Fusarium wilt of tomato. International Journal of Botany 4:369-375.
• Munif, A., Hallmann, J., Sikora, R.A., 2013. The influence of endophytic bacteria on Meloidogyneincognita infection and tomato plant growth. International Society for Southeast Asian Agricultural Sciences 19(2): 68-74.
• Nash, S.M., Snyder, W.C., 1962. Quantitative estimations by plate counts of propagules of the bean root rot Fusarium in field soils. Phytopathology 52(6): 567-572.
• Nejad, P., Johnson, P.A., 2000. Endophytic bacteria induce growth promotion and wilt disease suppression in oilseed rape and tomato. Biological Control 18(3): 208-215.
• Ngamau, C.N., Matiru, V.N., Tani, A., Muthuri, C.W., 2012. Isolation and identification of endophytic bacteria of bananas (Musa spp.) in Kenya and their potential as biofertilizers for sustainable banana production. African Journal of Microbiology Research 6: 6414-6422.
• Nourozian, J., Etabarian, H.R., Khodakaramian, G., 2006. Biological control of Fusarium graminearum on wheat by antagonistic bacteria. Songklanakarin Journal of Science Technology 28: 29-38.
• Patel, H.A., Patel, R.K., Khristi, S.K., Parikh, K., Rajendran, G., 2012. Isolation and characterization of bacterial endophytes from Lycopersicon esculentum plant and their plant growth promoting characteristics. Nepal Journal of Biotechnology 2(1): 37-52.
• Qiu, M., Zhang, R., Xue, C., Zhang, S., Li, S., Zhang, L., Shen, Q., 2012. Application of bio-organic fertilizer can control Fusarium wilt of cucumber plants by regulating microbial community of rhizosphere soil. Biology and Fertility of Soils 48(7): 807-816.
• Ramyabharathi, S.A., Raguchander, T., 2014. Efficacy of secondary metabolites produced by Bacillus subtilis EPCO16 against tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici. Journal of Mycology and Plant Pathology 44(2): 148-153.
• Santoyo, G., Moreno-Hagelsieb, G., Orozco-Mosqueda, M.D.C., 2016. Plant growth-promoting bacteria endophytes. Microbiological Research 183: 92-99.
• Sharma, V.K., Nowak, J., 1998. Enhancement of verticillium wilt resistance in tomato transplants by in vitro co-culture of seedlings with a plant growth promoting rhizobacterium (Pseudomonas sp. strain PsJN). Canadian Journal of Microbiology 44(6): 528-536.
• Shekhar, N., Bhattacharya, D., Kumar, D., Gupta, R.K., 2006. Biocontrol of wood-rotting fungi with Streptomyces violaceusniger XL-2. Canadian Journal of Microbiology 52(9): 805-808.
• Smith, G.E., 1957. Inhibition of Fusarium oxysporum f. sp. lycopersici by a species of Micromonospora isolated from tomato. Phytopathology 47: 429-432.
• Sreevidya, M., Gopalkrishnan, S., 2016. Penicillium citrinum VF1-51 as biocontrol agent to control charcoal rot of Sorghum (Sorghum bicolor (L.) Moench). African Journal of Micobiology Research 10(19): 669-674.
• Sreevidya, M., Gopalkrishnan, S., Kudapa, H., Varshney, R.K., 2016. Exploring plat growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea. Brazilian Journal of Microbiology 47(1): 85-95.
• Swarupa, V., Pavitra, K., Shivashankara, K.S., Ravishankar, K.V., 2016. Omics-driven approaches in plant-microbe interaction. In: Microbial inoculants in sustainable agricultural productivity: Research perspectives, Singh, D.P., Singh, H.B., Prabha, R., (Eds.). Springer India,pp. 61-84.
• Syngenta, 2015. Tomato Sahel: Crops & Products. Available at [Access date : 01.10.2018]: http://www3.syngenta.com/country/es/sp/productos/Documents/ft/ft-tomate-sahel.pdf.
• Trejo-Estrada, S.R., Sepulveda, I., Crawford, D.L., 1998. In vitro and in vivo antagonism of Streptomyces violaceusniger YCED9 against fungal pathogens of turfgrass. World Journal of Microbiology and Biotechnology 14(6): 865-872.
• Vijayabharathi, R., Sathya, A., Gopalakrishnan, S., 2016. A renaissance in plant growth-promoting and biocontrol agents by endophytes. In: Microbial inoculants in sustainable agricultural productivity: Research perspectives, Singh, D.P., Singh, H.B., Prabha, R. (Eds.). Springer India. pp.37-60.
• Yang, C., Crowley, D.E., Menge, J.A., 2001. 16S rDNA fingerprinting of rhizosphere bacterial communities associated with healthy and Phytophthora infected avocado roots. FEMS Microbial Ecology 35(2):129-136.
• Ypadhyay, R.S., Rai, B., 1987. Studies on antagonism betweenFusarium udum Butler and root region microflora of pigeon-pea. Plant and Soil 101(1): 79-93.
• Zeilinager, S., Gupta, V.K., Dahms, T.E.S., Silva, R.N., Singh, H.B., Ypadhyay, R.S., Gomes, E.V., Tsui, C.K.M., Chandra, S., 2016. Friends or foes? Emerging insights from fungal interactions with plants. FEMS Microbiology Reviews 40(2): 182-207.
• Zhang, S., Raza, W., Yang, X., Hu, J., Huang, Q., Xu, Y., Liu, X., Ran, W., Shen, Q., 2008. Control of Fusarium wilt disease of cucumber plants with the application of a bioorganic fertilizer. Biology and Fertility of Soils 44: 1073-1080.