Responses of salt-stressed citrus plants to foliar-applied proline

Year 2020, Volume: 9 Issue: 1, 66 - 74, 01.01.2020

Mehmet Ali Demiral Juan Carlos Melgar Beatriz Contreras Ayako Kusakabe Murat Uygun Seçil Küçük Kaya

https://doi.org/10.18393/ejss.647020

Abstract

In
this study, one-year old grapefruit trees grafted onto sour Orange (SO) and C22
rootstocks were exposed to NaCl-induced salinity (approx. 6 dS m^-1)
in pot culture for two months under greenhouse conditions. The experiment was
laid out in a randomized block design with eight replicates. The trees were
irrigated with saline solution containing 0.1% liquid fertilizer “Miracle-Gro
Liqua Feed 9-4-9” (N-P₂O₅-K₂O) enriched with
micronutrients. The experimental treatments consisted of three levels (4 mM, 8
mM, 12 mM) of foliar applied proline along with control application. Distilled
water served as the control. During the experiment the seedlings were sprayed
totally five times with ten days intervals. At the end of the treatment
physiologically mature leaves, free of damage or defects, were sampled. Dried
and ground leaf samples were used for chemical (Na and Cl) and biochemical
(DPPH scavenging activity, reducing power, total phenolic content, proline)
analysis. Spesific leaf area, leaf water relations and leaf gas exchange of the
plants were also determined. Foliar
PRO application decreased Na and Cl concentrations of the leaves, and improved
spesific leaf area in the final dose. Water leaf relations, photosynthetic
activity and biochemical parameters were affected positively even though some
differences were determined between the cultivars.

Keywords

Salinity, proline, NaCl, C22, SO

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