KASIB spring common wheat genotype identification on glutenin and gliadin subunits

Abstract

Based on the electrophoretic spectrum, analysis, 216 genotypes of spring wheat were identified as belonging to 25 types including 18 types of HMW-glutenin for 188 homogeneous cultivars. Most of the spring wheat KASIB could be characterized by the subunits 2 * 7+9 5+10 (40% genotypes), and subunits 2* 7+9 2+12– (30%) and subunits 1*7+9 5+10– (8%). The diversity appears to be determined by varying chromosome 1A and 1B at the 5 subunits and 1D-4 subunits HMG. Judging from the distribution of genotypes KASIB nurseries are mostly HMW-glutenin determining high quality baking by subunits: 1A-2 *(77-79%) and 1 (14-15%), at 1B-7+9 (81-82%) and 7+8(13-15%), by 1D –5+10 to 60%. A new subunit is to be included to this set ie., “4+10” for 1D for samples from Erythrospermum 55/9401-20 , Fiton 41 relative to genofund of high-quality genotypes found in Kazakhstan. Cultivar “Iridost” is identified with alleles 5.5+10 on chromosome 1D, previously identified for cultivars Tselinogradka, Tselinnaya 24, Akmola 3. In cultivar Chelyaba, chromosome 1B set possessed only 6+8 subunit and in the mix for cultivars Lutescens 29-94; for genotypes: E-607 and E-757 - a rare characteristic subunit7*+8 was found. Genotypes OK -1, Fiton 25 and GVK 1916-9 carrying wheat-rye 1B/1R-translocation clustered distantly from all other genotypes. Wheat-rye translocation (IB/ IR) were identified more in samples for Omsk breeding and West Kazakhstan in each KASIB , for all samples submitted “Kurgansemena” (except Lutescens415/00) and “Agrosemkonsalt” , as well as for LLP “Fiton” breeding samples (Fiton 41 and Fiton 43, including CIMMYT shuttle breeding material

Keywords

• spring wheat, the HMW and the LMW glutenin, gliadin, wheat-rye translocation, hardness, varieties identification, UPOV

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