Development of BAC-End based simple sequence repeat (SSR) markers in apple

Elmira Ziya Motalebipour; Nergiz Coban; Mortaza Khodaeiaminjan; Murat Guney; Serif Ozongun; Nilgun Atay; Salih Kafkas

EN

Development of BAC-End based simple sequence repeat (SSR) markers in apple

Abstract

A genome-wide bacterial artificial chromosome (BAC) physical map of the apple, Malus domestica Borkh., has been recently developed. This study addresses development of SSR markers from the BAC-end sequences in apple. Previously designed 187 BAC-SSR primer pairs were subjected firstly to gradient-PCR for amplification and to determine their optimum annealing temperatures. Then, the amplified primer pairs were tested in two F1 segregating populations: ‘Kasel-37’ x ‘Delbarestivale’, and ‘Kasel-41’ X ‘Williams Pride’. The PCR reactions were performed in 12 F1 progenies and in the parents to determine segregation types of the primer pairs. There was no amplification in 20 SSR primer pairs, and 87 of them were monomorphic in the two populations. As a result, 80 of the primer pairs showed segregation in each of the two F1 populations

Keywords

SSR, apple, polymorphism, microsatellite

Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Elmira Ziya Motalebipour This is me

Nergiz Coban This is me

Mortaza Khodaeiaminjan This is me

Murat Guney This is me

Serif Ozongun This is me

Nilgun Atay This is me

Salih Kafkas This is me

Publication Date

July 1, 2015

Submission Date

July 25, 2016

Acceptance Date

-

Published in Issue

Year 2015 Volume: 1 Number: 2

IZ

https://izlik.org/JA78RT78AE

Cite

RIS / Bibtex

APA

Motalebipour, E. Z., Coban, N., Khodaeiaminjan, M., Guney, M., Ozongun, S., Atay, N., & Kafkas, S. (2015). Development of BAC-End based simple sequence repeat (SSR) markers in apple. Ekin Journal of Crop Breeding and Genetics, 1(2), 84-92. https://izlik.org/JA78RT78AE

AMA

1.Motalebipour EZ, Coban N, Khodaeiaminjan M, et al. Development of BAC-End based simple sequence repeat (SSR) markers in apple. Ekin Journal. 2015;1(2):84-92. https://izlik.org/JA78RT78AE

Chicago

Motalebipour, Elmira Ziya, Nergiz Coban, Mortaza Khodaeiaminjan, et al. 2015. “Development of BAC-End Based Simple Sequence Repeat (SSR) Markers in Apple”. Ekin Journal of Crop Breeding and Genetics 1 (2): 84-92. https://izlik.org/JA78RT78AE.

EndNote

Motalebipour EZ, Coban N, Khodaeiaminjan M, Guney M, Ozongun S, Atay N, Kafkas S (July 1, 2015) Development of BAC-End based simple sequence repeat (SSR) markers in apple. Ekin Journal of Crop Breeding and Genetics 1 2 84–92.

IEEE

[1]E. Z. Motalebipour et al., “Development of BAC-End based simple sequence repeat (SSR) markers in apple”, Ekin Journal, vol. 1, no. 2, pp. 84–92, July 2015, [Online]. Available: https://izlik.org/JA78RT78AE

ISNAD

Motalebipour, Elmira Ziya - Coban, Nergiz - Khodaeiaminjan, Mortaza - Guney, Murat - Ozongun, Serif - Atay, Nilgun - Kafkas, Salih. “Development of BAC-End Based Simple Sequence Repeat (SSR) Markers in Apple”. Ekin Journal of Crop Breeding and Genetics 1/2 (July 1, 2015): 84-92. https://izlik.org/JA78RT78AE.

JAMA

1.Motalebipour EZ, Coban N, Khodaeiaminjan M, Guney M, Ozongun S, Atay N, Kafkas S. Development of BAC-End based simple sequence repeat (SSR) markers in apple. Ekin Journal. 2015;1:84–92.

MLA

Motalebipour, Elmira Ziya, et al. “Development of BAC-End Based Simple Sequence Repeat (SSR) Markers in Apple”. Ekin Journal of Crop Breeding and Genetics, vol. 1, no. 2, July 2015, pp. 84-92, https://izlik.org/JA78RT78AE.

Vancouver

1.Elmira Ziya Motalebipour, Nergiz Coban, Mortaza Khodaeiaminjan, Murat Guney, Serif Ozongun, Nilgun Atay, Salih Kafkas. Development of BAC-End based simple sequence repeat (SSR) markers in apple. Ekin Journal [Internet]. 2015 Jul. 1;1(2):84-92. Available from: https://izlik.org/JA78RT78AE